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The map label for this gene is bcr [H]

Identifier: 170750046

GI number: 170750046

Start: 3880538

End: 3881698

Strand: Reverse

Name: bcr [H]

Synonym: Mrad2831_3647

Alternate gene names: 170750046

Gene position: 3881698-3880538 (Counterclockwise)

Preceding gene: 170750047

Following gene: 170750043

Centisome position: 63.87

GC content: 74.25

Gene sequence:

>1161_bases
GTGGCGGAGCGCCGGGCACCCCTGCCGCTCCTGGTCGCCGTGACCATGACCGGAACGGTGGCGCTGCACATCTTCGTGCC
GGCGCTGGCGGCGGCGGCCGTCGACCTCGGCACCAACGCCGCCGGCGCCCAGCTGACGATCACCGTCTACCTGATCGGGC
TCGCGGGCGGTCAGCTCCTCTATGGGCCGCTCTCGGACCGGTACGGGCGGCGCCCGATCCTGATCGGGGGACTCGGCCTC
TACCTCGCCGGGCTGCTGCTCGCGATCCCCGCGCCGAATATCGGCGTCCTGCTCGTCGCCCGTGTCCTGCAGTCGCTCGG
CGCCTGCGGCTCGCTGGTGATCGGCCGGGCGATGGTCCGCGACGTCTCGACCCGCGAGGACGCGGCCCGGAAGCTCGCGA
TCCTCACCACCGCGATGACGCTCACGCCCGCGCTGGCCCCGGCGATCGGCGGCGTGGTGAACGAGGCTTTCGGCTGGCGC
GCGGTGTTCGTGGTGCTGGCCGGCCTCGTCGCCGTGCTGGGCGCGCTCGTGGTCTTCACCCTGCCCGAGACCAACCGCCA
CAAGGTCGCGCTGCCGGGCTTCGTCGCCGTTCTGTCCGGCTACCTGCGGCTGGTGCGCGTCCCGGTGTTCCGCGCCTACC
TGGTGGCGGGGGCCTGCGGCGGCACCAGCCTCTACGCCTTCCTGGCCGTGGCGCCGTTCCTCCTCGTGGACCGGCTCGGG
CGCTCCTCGCAGGAGGTCGGGTTCGACTGCCTGATCGTCGTGTTCGGCATGGTCGGCGGGGCCGTCCTGGCGAGCCGGCT
CGCCGCCCGGGTGCCGATCCGCAAGGCGGCGCGGAGCGGCAACCTCCTCTGCATCGGCGCGGCGCTGTCGCTGCTGCTCG
TGGACCGCACCGGCATGCTCGGCGTGGTCAGCCTGATCGTGCCGCTCTTCGCCTACGCGGTGGGCGTCGGCCTGCTCAGC
CCCAACGCGGTGGCGGGGCTGATGAACGTCGACCCGCACGCGGCCGGCTCGGCGTCCAGCCTCTACGGCTTCACGCAGAT
GACCTTCGGGGCGCTGTTCACGGTCGCCGCCTCCGCGTGGCCCGCGACCTCGGCGACGCCGGTGGCGCTCGCCCTGCTCG
CGGCCGGCCTGATCGCCGCGCTGGCGCTCCGGCGGGCCTGA

Upstream 100 bases:

>100_bases
GCATGGCTCCGGGCGCGGCCCTTGCTCTAAGGCGGCCGCGCCCCGGCATCCTGCCGGATCGAGAACCGCGTAGGACCCCT
TGGCTGATCGAAGTCCCGAT

Downstream 100 bases:

>100_bases
CGGGACCGCGCGCGACGGCGGCGCGGGACCGTCTCCGTCACCGCGTCGGGCCGAGCGCCTCAACGGCGCCCGACGCGCCG
GCGCCGAAACCGTCGGCCAG

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MAERRAPLPLLVAVTMTGTVALHIFVPALAAAAVDLGTNAAGAQLTITVYLIGLAGGQLLYGPLSDRYGRRPILIGGLGL
YLAGLLLAIPAPNIGVLLVARVLQSLGACGSLVIGRAMVRDVSTREDAARKLAILTTAMTLTPALAPAIGGVVNEAFGWR
AVFVVLAGLVAVLGALVVFTLPETNRHKVALPGFVAVLSGYLRLVRVPVFRAYLVAGACGGTSLYAFLAVAPFLLVDRLG
RSSQEVGFDCLIVVFGMVGGAVLASRLAARVPIRKAARSGNLLCIGAALSLLLVDRTGMLGVVSLIVPLFAYAVGVGLLS
PNAVAGLMNVDPHAAGSASSLYGFTQMTFGALFTVAASAWPATSATPVALALLAAGLIAALALRRA

Sequences:

>Translated_386_residues
MAERRAPLPLLVAVTMTGTVALHIFVPALAAAAVDLGTNAAGAQLTITVYLIGLAGGQLLYGPLSDRYGRRPILIGGLGL
YLAGLLLAIPAPNIGVLLVARVLQSLGACGSLVIGRAMVRDVSTREDAARKLAILTTAMTLTPALAPAIGGVVNEAFGWR
AVFVVLAGLVAVLGALVVFTLPETNRHKVALPGFVAVLSGYLRLVRVPVFRAYLVAGACGGTSLYAFLAVAPFLLVDRLG
RSSQEVGFDCLIVVFGMVGGAVLASRLAARVPIRKAARSGNLLCIGAALSLLLVDRTGMLGVVSLIVPLFAYAVGVGLLS
PNAVAGLMNVDPHAAGSASSLYGFTQMTFGALFTVAASAWPATSATPVALALLAAGLIAALALRRA
>Mature_385_residues
AERRAPLPLLVAVTMTGTVALHIFVPALAAAAVDLGTNAAGAQLTITVYLIGLAGGQLLYGPLSDRYGRRPILIGGLGLY
LAGLLLAIPAPNIGVLLVARVLQSLGACGSLVIGRAMVRDVSTREDAARKLAILTTAMTLTPALAPAIGGVVNEAFGWRA
VFVVLAGLVAVLGALVVFTLPETNRHKVALPGFVAVLSGYLRLVRVPVFRAYLVAGACGGTSLYAFLAVAPFLLVDRLGR
SSQEVGFDCLIVVFGMVGGAVLASRLAARVPIRKAARSGNLLCIGAALSLLLVDRTGMLGVVSLIVPLFAYAVGVGLLSP
NAVAGLMNVDPHAAGSASSLYGFTQMTFGALFTVAASAWPATSATPVALALLAAGLIAALALRRA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=352, Percent_Identity=28.125, Blast_Score=137, Evalue=8e-34,
Organism=Escherichia coli, GI48994889, Length=365, Percent_Identity=29.5890410958904, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082312, Length=329, Percent_Identity=30.3951367781155, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790146, Length=154, Percent_Identity=33.1168831168831, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1790794, Length=310, Percent_Identity=26.7741935483871, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787065, Length=327, Percent_Identity=25.0764525993884, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081738, Length=188, Percent_Identity=28.1914893617021, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6319480, Length=182, Percent_Identity=28.021978021978, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324264, Length=197, Percent_Identity=26.3959390862944, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321838, Length=145, Percent_Identity=29.6551724137931, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=189, Percent_Identity=28.042328042328, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 39323; Mature: 39191

Theoretical pI: Translated: 10.72; Mature: 10.72

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAERRAPLPLLVAVTMTGTVALHIFVPALAAAAVDLGTNAAGAQLTITVYLIGLAGGQLL
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEE
YGPLSDRYGRRPILIGGLGLYLAGLLLAIPAPNIGVLLVARVLQSLGACGSLVIGRAMVR
ECCCHHCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVSTREDAARKLAILTTAMTLTPALAPAIGGVVNEAFGWRAVFVVLAGLVAVLGALVVFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LPETNRHKVALPGFVAVLSGYLRLVRVPVFRAYLVAGACGGTSLYAFLAVAPFLLVDRLG
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
RSSQEVGFDCLIVVFGMVGGAVLASRLAARVPIRKAARSGNLLCIGAALSLLLVDRTGML
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHHHHHCCCCHH
GVVSLIVPLFAYAVGVGLLSPNAVAGLMNVDPHAAGSASSLYGFTQMTFGALFTVAASAW
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PATSATPVALALLAAGLIAALALRRA
CCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AERRAPLPLLVAVTMTGTVALHIFVPALAAAAVDLGTNAAGAQLTITVYLIGLAGGQLL
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCEE
YGPLSDRYGRRPILIGGLGLYLAGLLLAIPAPNIGVLLVARVLQSLGACGSLVIGRAMVR
ECCCHHCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVSTREDAARKLAILTTAMTLTPALAPAIGGVVNEAFGWRAVFVVLAGLVAVLGALVVFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LPETNRHKVALPGFVAVLSGYLRLVRVPVFRAYLVAGACGGTSLYAFLAVAPFLLVDRLG
CCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
RSSQEVGFDCLIVVFGMVGGAVLASRLAARVPIRKAARSGNLLCIGAALSLLLVDRTGML
CCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHHHHHCCCCHH
GVVSLIVPLFAYAVGVGLLSPNAVAGLMNVDPHAAGSASSLYGFTQMTFGALFTVAASAW
HHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PATSATPVALALLAAGLIAALALRRA
CCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]