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The map label for this gene is dppA [H]

Identifier: 170750001

GI number: 170750001

Start: 3830017

End: 3831636

Strand: Direct

Name: dppA [H]

Synonym: Mrad2831_3602

Alternate gene names: 170750001

Gene position: 3830017-3831636 (Clockwise)

Preceding gene: 170750000

Following gene: 170750002

Centisome position: 63.02

GC content: 70.06

Gene sequence:

>1620_bases
ATGGACCCGATCAAGCCGACACGCCGATCCCTGATGGCCGGGGGCGTCGCCCTCGCGGCGCTGGGAGCGGCGGGCCGGGC
CGGCGCGCAGGGCGCGGCCCCCGGCACCCTGACCTACGGCATCTCCATGACCGACCTGCCGCTGACCACCGGGCAGCCGG
ACCGGGGCGCGGGCGGCTACCAGTTCACCGGCCTGACCCTCTACGACCCCCTGGTCGCCTGGGAGCTCGACGTCGCCGAC
CGGCCCGGCAAGATGGTGCCGGGCCTCGCCACCGCGTGGGAGGCCGACCCGGCCGACCCCAAGGTCTGGACCTTCCACCT
GCGCGAGGGCGTGGCCTTCCACGACGGCTCGGCCTTCGATGCCGACGCGGTGATCTGGAACTTCGACAAGGTGCTGAACA
AGGAGGCGCCGCATTTCGACCAGCGGCAGGCCGCCCAGGTCCGCCCGCGCCTGCCCGGGGTCGCGAGCTACCGGAAGCTC
GACGCCAAGACCGTCCAGGTCGTCACCAAGACGCCCGACAGCCTGTTCCCCTACCAGATGCTCTGGTTCCTGATCTCGTC
GCCCGCGCAGTACGAGAAGGTCGGGAAGGACTGGGCGAAGTTCGCCTTCGAGCCCTCGGGAACCGGGCCGTTCCGGATGG
GGCAGCTGGTGCCGCGGGTGCGCCTCGAGCTGCTGCCGAACGTCGGCTACTGGAACCCGAAGCGGGTCCCGAAGCTCGCG
AAGCTGACCCTCACCTGCGTGCCCGAGGATCTCGCGCGGGTGAACGCGCTCCTGTCCGGCAGCGTCGACCTGATCGAGGC
GCCCGCGCCCGACGCGGTGCCGCGCCTGAAGGCCGCCGGCATGCGGGTCGTGGGCAACGAGACGCCCCATGTCTGGAACT
ACCACCTGTCGATGCTGGAGGGATCGCCCTGGCGCGACCTGCGCCTGCGCAAGGCCGCCAACCTCGCCATCGACCGCGCG
GGCGTCGTCGAGCTGATGGGCGGTCTCGCCCAGCCCGCGGTCGGGCAGGTCCAGGAGTCGAGCCCGTGGTTCGGCAAGCC
GGGCTTCAGGATCCGGACCGACGTCGAGGCCGCGCGCAAGCTCGTCGAGGAGGCCGGCTACTCGGTGAAGAACCCCGTGA
AGGCCACGGTCCTGATCCCGACCGGCGGCACCGGGCAGATGCTGTCGCTGCCGATCAACGAGTTCGTCCAGCAGAGCTGG
GCGGAGATCGGGATCCATGTGGAGTTCAAGACCGTCGAGCTGGAGGTCGCCTACACGGCGTGGCGCCAGGGCGCCGCGGA
TCCGAGCCTCAAGGGCGTGAGCGCCGGCAACATCGCCTACGTCACGTCCGACCCGTTCTACGCGATCCTGCGCTTCTACG
ATTCGCGTCAGATCGCGCCGAACGGCGTGAACTGGAGCCACTACCGCAACCCCGAGGTCGACGCCCTCTGCGACAAGGTG
AAGGCGAGCCTCGACGTGGCCGAGCAGAACCGGCTGCTCGCGCGCATCCACGAAATCGTCGTCGACGACGCCGTGCAGGT
CTGGGTGGTTCACGACACCAATCCGCACGCGCTGTCGTCGCGGGTGAAGAATTACGTCCAGGCGCAGCACTGGTTTCAGG
ACCTCGCGACGCTCGCCTGA

Upstream 100 bases:

>100_bases
TTCTGTATGCAGCATGGGGAGGAGGCTGGGGCAGCGCCGTCGCGCGCCTGACGGCCGGTGCCGCTCCGTGCGGCGCCCGC
GCCTTCAGCCGAGAGACACG

Downstream 100 bases:

>100_bases
TCCATCCGGAACTGTGAGGCGCGCGGGAAGAACGGGAGAACATCGGGAACGATCGGCGCGAAGACGGGTTGGACCCCGAA
CGCACGAAGGAGAGTCCCAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MDPIKPTRRSLMAGGVALAALGAAGRAGAQGAAPGTLTYGISMTDLPLTTGQPDRGAGGYQFTGLTLYDPLVAWELDVAD
RPGKMVPGLATAWEADPADPKVWTFHLREGVAFHDGSAFDADAVIWNFDKVLNKEAPHFDQRQAAQVRPRLPGVASYRKL
DAKTVQVVTKTPDSLFPYQMLWFLISSPAQYEKVGKDWAKFAFEPSGTGPFRMGQLVPRVRLELLPNVGYWNPKRVPKLA
KLTLTCVPEDLARVNALLSGSVDLIEAPAPDAVPRLKAAGMRVVGNETPHVWNYHLSMLEGSPWRDLRLRKAANLAIDRA
GVVELMGGLAQPAVGQVQESSPWFGKPGFRIRTDVEAARKLVEEAGYSVKNPVKATVLIPTGGTGQMLSLPINEFVQQSW
AEIGIHVEFKTVELEVAYTAWRQGAADPSLKGVSAGNIAYVTSDPFYAILRFYDSRQIAPNGVNWSHYRNPEVDALCDKV
KASLDVAEQNRLLARIHEIVVDDAVQVWVVHDTNPHALSSRVKNYVQAQHWFQDLATLA

Sequences:

>Translated_539_residues
MDPIKPTRRSLMAGGVALAALGAAGRAGAQGAAPGTLTYGISMTDLPLTTGQPDRGAGGYQFTGLTLYDPLVAWELDVAD
RPGKMVPGLATAWEADPADPKVWTFHLREGVAFHDGSAFDADAVIWNFDKVLNKEAPHFDQRQAAQVRPRLPGVASYRKL
DAKTVQVVTKTPDSLFPYQMLWFLISSPAQYEKVGKDWAKFAFEPSGTGPFRMGQLVPRVRLELLPNVGYWNPKRVPKLA
KLTLTCVPEDLARVNALLSGSVDLIEAPAPDAVPRLKAAGMRVVGNETPHVWNYHLSMLEGSPWRDLRLRKAANLAIDRA
GVVELMGGLAQPAVGQVQESSPWFGKPGFRIRTDVEAARKLVEEAGYSVKNPVKATVLIPTGGTGQMLSLPINEFVQQSW
AEIGIHVEFKTVELEVAYTAWRQGAADPSLKGVSAGNIAYVTSDPFYAILRFYDSRQIAPNGVNWSHYRNPEVDALCDKV
KASLDVAEQNRLLARIHEIVVDDAVQVWVVHDTNPHALSSRVKNYVQAQHWFQDLATLA
>Mature_539_residues
MDPIKPTRRSLMAGGVALAALGAAGRAGAQGAAPGTLTYGISMTDLPLTTGQPDRGAGGYQFTGLTLYDPLVAWELDVAD
RPGKMVPGLATAWEADPADPKVWTFHLREGVAFHDGSAFDADAVIWNFDKVLNKEAPHFDQRQAAQVRPRLPGVASYRKL
DAKTVQVVTKTPDSLFPYQMLWFLISSPAQYEKVGKDWAKFAFEPSGTGPFRMGQLVPRVRLELLPNVGYWNPKRVPKLA
KLTLTCVPEDLARVNALLSGSVDLIEAPAPDAVPRLKAAGMRVVGNETPHVWNYHLSMLEGSPWRDLRLRKAANLAIDRA
GVVELMGGLAQPAVGQVQESSPWFGKPGFRIRTDVEAARKLVEEAGYSVKNPVKATVLIPTGGTGQMLSLPINEFVQQSW
AEIGIHVEFKTVELEVAYTAWRQGAADPSLKGVSAGNIAYVTSDPFYAILRFYDSRQIAPNGVNWSHYRNPEVDALCDKV
KASLDVAEQNRLLARIHEIVVDDAVQVWVVHDTNPHALSSRVKNYVQAQHWFQDLATLA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=569, Percent_Identity=23.3743409490334, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1787495, Length=557, Percent_Identity=23.6983842010772, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1787052, Length=470, Percent_Identity=25.9574468085106, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789887, Length=429, Percent_Identity=25.4079254079254, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87081878, Length=478, Percent_Identity=21.7573221757322, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789397, Length=448, Percent_Identity=22.0982142857143, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787551, Length=386, Percent_Identity=23.3160621761658, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58996; Mature: 58996

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDPIKPTRRSLMAGGVALAALGAAGRAGAQGAAPGTLTYGISMTDLPLTTGQPDRGAGGY
CCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
QFTGLTLYDPLVAWELDVADRPGKMVPGLATAWEADPADPKVWTFHLREGVAFHDGSAFD
EECCEEEECCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCEEECCCCCCC
ADAVIWNFDKVLNKEAPHFDQRQAAQVRPRLPGVASYRKLDAKTVQVVTKTPDSLFPYQM
CCCEEEEHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCHHHH
LWFLISSPAQYEKVGKDWAKFAFEPSGTGPFRMGQLVPRVRLELLPNVGYWNPKRVPKLA
HHHHHCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
KLTLTCVPEDLARVNALLSGSVDLIEAPAPDAVPRLKAAGMRVVGNETPHVWNYHLSMLE
EEEEEECCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCEEEECCCCCCEEEEEEEEEC
GSPWRDLRLRKAANLAIDRAGVVELMGGLAQPAVGQVQESSPWFGKPGFRIRTDVEAARK
CCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHH
LVEEAGYSVKNPVKATVLIPTGGTGQMLSLPINEFVQQSWAEIGIHVEFKTVELEVAYTA
HHHHCCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHEEEEEEEEEEEEEEEEHH
WRQGAADPSLKGVSAGNIAYVTSDPFYAILRFYDSRQIAPNGVNWSHYRNPEVDALCDKV
HHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
KASLDVAEQNRLLARIHEIVVDDAVQVWVVHDTNPHALSSRVKNYVQAQHWFQDLATLA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDPIKPTRRSLMAGGVALAALGAAGRAGAQGAAPGTLTYGISMTDLPLTTGQPDRGAGGY
CCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCE
QFTGLTLYDPLVAWELDVADRPGKMVPGLATAWEADPADPKVWTFHLREGVAFHDGSAFD
EECCEEEECCEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCEEEEEECCCEEECCCCCCC
ADAVIWNFDKVLNKEAPHFDQRQAAQVRPRLPGVASYRKLDAKTVQVVTKTPDSLFPYQM
CCCEEEEHHHHHCCCCCCCCHHHHHHHCCCCCCHHHHHHCCCEEEEEEECCCCCCCHHHH
LWFLISSPAQYEKVGKDWAKFAFEPSGTGPFRMGQLVPRVRLELLPNVGYWNPKRVPKLA
HHHHHCCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
KLTLTCVPEDLARVNALLSGSVDLIEAPAPDAVPRLKAAGMRVVGNETPHVWNYHLSMLE
EEEEEECCHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCCEEEECCCCCCEEEEEEEEEC
GSPWRDLRLRKAANLAIDRAGVVELMGGLAQPAVGQVQESSPWFGKPGFRIRTDVEAARK
CCCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHH
LVEEAGYSVKNPVKATVLIPTGGTGQMLSLPINEFVQQSWAEIGIHVEFKTVELEVAYTA
HHHHCCCCCCCCCEEEEEEECCCCCCEEECCHHHHHHHHHHHEEEEEEEEEEEEEEEEHH
WRQGAADPSLKGVSAGNIAYVTSDPFYAILRFYDSRQIAPNGVNWSHYRNPEVDALCDKV
HHCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
KASLDVAEQNRLLARIHEIVVDDAVQVWVVHDTNPHALSSRVKNYVQAQHWFQDLATLA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]