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The map label for this gene is hipO [H]

Identifier: 170749983

GI number: 170749983

Start: 3809027

End: 3810199

Strand: Reverse

Name: hipO [H]

Synonym: Mrad2831_3584

Alternate gene names: 170749983

Gene position: 3810199-3809027 (Counterclockwise)

Preceding gene: 170749984

Following gene: 170749982

Centisome position: 62.69

GC content: 71.53

Gene sequence:

>1173_bases
ATGCCCGTCCTCGAACGGATCCACGCCTTCGCCGACACGATGACGGCGTGGCGGCACGACCTGCACGCCCATCCCGAGCT
GCTCTACGACGTCGACCGCACCGCCGGCTTCGTCGCCGAGACGCTGCGGTCCTTCGGCTGCGACGAGGTCGTTACCGGTA
TCGGCCGCACCGGGGTGGTCGGCGTCATCCGCGGCCGGGGACGCAGCTCGAACCGCGCCATCGGCCTGCGCGCCGACATG
GACGCCCTGCCGATCCAGGAGGTGCGCGACCTGCCCTACCGCTCGACCGTGCCGGGCAAGATGCACGCCTGCGGCCACGA
CGGGCACACCGCCATGCTGCTCGGCGCCGCCAAGTACCTCGCCGAGACCCGGGATTTCGACGGGGCGGCCGTGCTGATCT
TCCAGCCCGCCGAGGAGGGCGGCGGCGGCGGCGAGGCCATGGTCGACGACGGCCTGATGGAGCGGTTCGGGGTCGAGTCG
GTCTACGGGATGCACAACATCCCCGGCATGGCGGTCGGCACCTTCGCGATCCGGCCGGGGCCGATCATGGCCTCGACCGA
CCGCTTCACCGTCACGATCGAGGGACGGGGCGGGCATGCGGCCCTGCCGCAGAACGCCGTCGACAGCGTGCTCGTGGCGA
GCCACGTCATCATCGCCCTGCAGAGCATCGTGTCGCGCAACCTCGACCCAGTGCAGTCGGCCGTCGTCTCGGTCTGCGCG
CTGGAGGCCGGCGAGGCCTTCAACGTGCTTCCGCAGACCGTCACCATGCGGGGCACGATGCGCACCCTGTCCCAGGCCGT
GCGCAGCCAGATCAAGGCGCGGATCGAGACCCTGGTGGCCGAGATCGCCGGCGGCTTCGGCGCGCGGGGCCGGGTCGAGT
ACGGGGCGAGCTACCCGGTGACCGAGAACCATCCCGCCGAGACCGACTTCATGGCCGGCGTCGCCGCGGCGCTGGTAGGT
GGGGAGAACGTCGACCGGGCGGTGGCGCCGATGATGACCGCCGAGGACTTCTCCTACATGCTCGGCCGCCGCCCCGGCGC
GTACATGTTCATCGGCAACGGCGACAGTGCCTCGCTCCACCACCCGCACTACGACTTCAACGACGCGGCCGCGCCGTACG
GGGCTTCCCTGTGGGCGCGCCTGATCGAGACCGGGCTGCCGCGCAGCGCCTGA

Upstream 100 bases:

>100_bases
GGGCGATCCGCGCGCTCGCCAAGCGTGGCCGTTGACGGCGGCTCTGGCCCCGGCTTTGCTGGTCCGACCCACCCGCAAAG
GCCACGAGAGCCCCGACCCG

Downstream 100 bases:

>100_bases
GCGCGCCGCCCGCCGCCCCGTCGCTGGCCGAGGCCGTCCCGGTCTGGCTGCGGGTGGCGGCGCTCTCCTTCGGCGGGCCG
GCCGGGCAGATCGCCGTGAT

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 390; Mature: 389

Protein sequence:

>390_residues
MPVLERIHAFADTMTAWRHDLHAHPELLYDVDRTAGFVAETLRSFGCDEVVTGIGRTGVVGVIRGRGRSSNRAIGLRADM
DALPIQEVRDLPYRSTVPGKMHACGHDGHTAMLLGAAKYLAETRDFDGAAVLIFQPAEEGGGGGEAMVDDGLMERFGVES
VYGMHNIPGMAVGTFAIRPGPIMASTDRFTVTIEGRGGHAALPQNAVDSVLVASHVIIALQSIVSRNLDPVQSAVVSVCA
LEAGEAFNVLPQTVTMRGTMRTLSQAVRSQIKARIETLVAEIAGGFGARGRVEYGASYPVTENHPAETDFMAGVAAALVG
GENVDRAVAPMMTAEDFSYMLGRRPGAYMFIGNGDSASLHHPHYDFNDAAAPYGASLWARLIETGLPRSA

Sequences:

>Translated_390_residues
MPVLERIHAFADTMTAWRHDLHAHPELLYDVDRTAGFVAETLRSFGCDEVVTGIGRTGVVGVIRGRGRSSNRAIGLRADM
DALPIQEVRDLPYRSTVPGKMHACGHDGHTAMLLGAAKYLAETRDFDGAAVLIFQPAEEGGGGGEAMVDDGLMERFGVES
VYGMHNIPGMAVGTFAIRPGPIMASTDRFTVTIEGRGGHAALPQNAVDSVLVASHVIIALQSIVSRNLDPVQSAVVSVCA
LEAGEAFNVLPQTVTMRGTMRTLSQAVRSQIKARIETLVAEIAGGFGARGRVEYGASYPVTENHPAETDFMAGVAAALVG
GENVDRAVAPMMTAEDFSYMLGRRPGAYMFIGNGDSASLHHPHYDFNDAAAPYGASLWARLIETGLPRSA
>Mature_389_residues
PVLERIHAFADTMTAWRHDLHAHPELLYDVDRTAGFVAETLRSFGCDEVVTGIGRTGVVGVIRGRGRSSNRAIGLRADMD
ALPIQEVRDLPYRSTVPGKMHACGHDGHTAMLLGAAKYLAETRDFDGAAVLIFQPAEEGGGGGEAMVDDGLMERFGVESV
YGMHNIPGMAVGTFAIRPGPIMASTDRFTVTIEGRGGHAALPQNAVDSVLVASHVIIALQSIVSRNLDPVQSAVVSVCAL
EAGEAFNVLPQTVTMRGTMRTLSQAVRSQIKARIETLVAEIAGGFGARGRVEYGASYPVTENHPAETDFMAGVAAALVGG
ENVDRAVAPMMTAEDFSYMLGRRPGAYMFIGNGDSASLHHPHYDFNDAAAPYGASLWARLIETGLPRSA

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=413, Percent_Identity=27.3607748184019, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41461; Mature: 41329

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLERIHAFADTMTAWRHDLHAHPELLYDVDRTAGFVAETLRSFGCDEVVTGIGRTGVV
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRGRSSNRAIGLRADMDALPIQEVRDLPYRSTVPGKMHACGHDGHTAMLLGAAKYL
EEEECCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AETRDFDGAAVLIFQPAEEGGGGGEAMVDDGLMERFGVESVYGMHNIPGMAVGTFAIRPG
HHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCC
PIMASTDRFTVTIEGRGGHAALPQNAVDSVLVASHVIIALQSIVSRNLDPVQSAVVSVCA
CCEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEAGEAFNVLPQTVTMRGTMRTLSQAVRSQIKARIETLVAEIAGGFGARGRVEYGASYPV
HCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
TENHPAETDFMAGVAAALVGGENVDRAVAPMMTAEDFSYMLGRRPGAYMFIGNGDSASLH
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
HPHYDFNDAAAPYGASLWARLIETGLPRSA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PVLERIHAFADTMTAWRHDLHAHPELLYDVDRTAGFVAETLRSFGCDEVVTGIGRTGVV
CHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEE
GVIRGRGRSSNRAIGLRADMDALPIQEVRDLPYRSTVPGKMHACGHDGHTAMLLGAAKYL
EEEECCCCCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
AETRDFDGAAVLIFQPAEEGGGGGEAMVDDGLMERFGVESVYGMHNIPGMAVGTFAIRPG
HHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEECCC
PIMASTDRFTVTIEGRGGHAALPQNAVDSVLVASHVIIALQSIVSRNLDPVQSAVVSVCA
CCEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
LEAGEAFNVLPQTVTMRGTMRTLSQAVRSQIKARIETLVAEIAGGFGARGRVEYGASYPV
HCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
TENHPAETDFMAGVAAALVGGENVDRAVAPMMTAEDFSYMLGRRPGAYMFIGNGDSASLH
CCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCC
HPHYDFNDAAAPYGASLWARLIETGLPRSA
CCCCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]