BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is yvaG [H]

Identifier: 170749921

GI number: 170749921

Start: 3736122

End: 3736919

Strand: Direct

Name: yvaG [H]

Synonym: Mrad2831_3521

Alternate gene names: 170749921

Gene position: 3736122-3736919 (Clockwise)

Preceding gene: 170749920

Following gene: 170749923

Centisome position: 61.47

GC content: 70.8

Gene sequence:

>798_bases
ATGGATCTCGATCTCACCGGCCGGAAGGCCCTGGTCACCGGCTCGACCGGCGGTATCGGCTACGCCATCGCCAAGGAACT
CTGCGATCTCGGCGCCGAGGTCGGCATCAACGGCCGCACGCCCGAGCGCGTCGAGGCAGCGCTGGCGAAGCTGCGGGGCG
AGGCCAGGGCCGGCCGGGCCTTCGCGGCGCCGGGTGATGTCGCGACCGCGGACGGCGTGTCCGCGCTCGTCCAGGCGCTG
CCGGCCGTGGACATCCTGGTCAACAACACCGGGATCTTCGAGCCGAAACCGTTCTTCGAGATCCCGGATGCCGACTGGCA
GCGCTTCTTCGACGTCAACGTGATGAGCGGCGTGCGCCTGTCCCGGGCCTACACGCCCGGCATGGCGGAGCGGGGCTGGG
GCCGGGTGATCTTCATCTCCAGCGAGTCCGGCATCAACACGCCGACCGAGATGGTCCATTACGGGATGACCAAGACCGCG
CAGCTCGCGATCTCCCGCGGGCTCGCCCAGACGGTGGCCGGGAGCGGCGTGACGGTCAACAGCGTCCTGCCCGGCCCGAC
CCTGTCGGAAGGTGTCGCCGAGTTCATGAAGCAGATGGCCGGCGGCGCGGCCGATGCCGACCTCGAAGCCATGGGCCGCA
AGTTCGTGGCGGAGCACCGGCCCTCCTCGCTGATCCAGCGCCTCGCGCGGGTGGAGGAGGTGGCGAACCTCGTTGCCTAC
CTCTGCAGCCCGGCCGCGAGCGCCACCACCGGCGCGGCTCTGCGGGTCGATGGCGGTGTCCTGCAGGGCCTCGCCTAG

Upstream 100 bases:

>100_bases
CCAGCGTCCTGCGGCGGAGTCGGAGCGGCTAGACCGGTCCCGTATGCCACCGCGGGCACCGGACCGCGTTGACGGAACGA
GTCACGTGCTGGAGCGTCCC

Downstream 100 bases:

>100_bases
GATCGGGCAGCACCTGGCATCGGGAGCAGGCTCGGCGCGGACGCGCCCGGCCGGTCGCCGGACGACCGGCCCTGTCCGCG
ACCCTGCGGGCGCGGGACGG

Product: short-chain dehydrogenase/reductase SDR

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDLDLTGRKALVTGSTGGIGYAIAKELCDLGAEVGINGRTPERVEAALAKLRGEARAGRAFAAPGDVATADGVSALVQAL
PAVDILVNNTGIFEPKPFFEIPDADWQRFFDVNVMSGVRLSRAYTPGMAERGWGRVIFISSESGINTPTEMVHYGMTKTA
QLAISRGLAQTVAGSGVTVNSVLPGPTLSEGVAEFMKQMAGGAADADLEAMGRKFVAEHRPSSLIQRLARVEEVANLVAY
LCSPAASATTGAALRVDGGVLQGLA

Sequences:

>Translated_265_residues
MDLDLTGRKALVTGSTGGIGYAIAKELCDLGAEVGINGRTPERVEAALAKLRGEARAGRAFAAPGDVATADGVSALVQAL
PAVDILVNNTGIFEPKPFFEIPDADWQRFFDVNVMSGVRLSRAYTPGMAERGWGRVIFISSESGINTPTEMVHYGMTKTA
QLAISRGLAQTVAGSGVTVNSVLPGPTLSEGVAEFMKQMAGGAADADLEAMGRKFVAEHRPSSLIQRLARVEEVANLVAY
LCSPAASATTGAALRVDGGVLQGLA
>Mature_265_residues
MDLDLTGRKALVTGSTGGIGYAIAKELCDLGAEVGINGRTPERVEAALAKLRGEARAGRAFAAPGDVATADGVSALVQAL
PAVDILVNNTGIFEPKPFFEIPDADWQRFFDVNVMSGVRLSRAYTPGMAERGWGRVIFISSESGINTPTEMVHYGMTKTA
QLAISRGLAQTVAGSGVTVNSVLPGPTLSEGVAEFMKQMAGGAADADLEAMGRKFVAEHRPSSLIQRLARVEEVANLVAY
LCSPAASATTGAALRVDGGVLQGLA

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=267, Percent_Identity=30.7116104868914, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI5031737, Length=262, Percent_Identity=32.824427480916, Blast_Score=104, Evalue=7e-23,
Organism=Homo sapiens, GI19923817, Length=263, Percent_Identity=29.277566539924, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI126723750, Length=263, Percent_Identity=28.1368821292776, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI7705855, Length=185, Percent_Identity=32.4324324324324, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI126723191, Length=190, Percent_Identity=32.1052631578947, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI33667109, Length=189, Percent_Identity=34.9206349206349, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI109715829, Length=252, Percent_Identity=28.1746031746032, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI7705925, Length=265, Percent_Identity=27.1698113207547, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4504505, Length=259, Percent_Identity=25.8687258687259, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI226371731, Length=188, Percent_Identity=28.7234042553192, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI10190704, Length=259, Percent_Identity=30.5019305019305, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI10190746, Length=210, Percent_Identity=29.5238095238095, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI7657478, Length=206, Percent_Identity=28.6407766990291, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI66933014, Length=199, Percent_Identity=25.1256281407035, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI40807363, Length=173, Percent_Identity=28.3236994219653, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI7706318, Length=198, Percent_Identity=25.2525252525253, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787905, Length=270, Percent_Identity=32.5925925925926, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1790717, Length=266, Percent_Identity=31.9548872180451, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1787335, Length=263, Percent_Identity=32.319391634981, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=261, Percent_Identity=30.6513409961686, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788459, Length=258, Percent_Identity=29.4573643410853, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI2367365, Length=255, Percent_Identity=30.9803921568627, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1786812, Length=265, Percent_Identity=30.5660377358491, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI87082160, Length=268, Percent_Identity=27.2388059701493, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787820, Length=180, Percent_Identity=32.7777777777778, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1789378, Length=261, Percent_Identity=29.8850574712644, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789208, Length=270, Percent_Identity=27.4074074074074, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI2367175, Length=259, Percent_Identity=30.1158301158301, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=33.8403041825095, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17560676, Length=262, Percent_Identity=30.5343511450382, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI193204405, Length=266, Percent_Identity=32.3308270676692, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17568967, Length=202, Percent_Identity=29.7029702970297, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI115534694, Length=263, Percent_Identity=30.7984790874525, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=259, Percent_Identity=30.5019305019305, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=29.296875, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25147288, Length=264, Percent_Identity=30.3030303030303, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=266, Percent_Identity=28.9473684210526, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=266, Percent_Identity=29.6992481203008, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=266, Percent_Identity=29.3233082706767, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=279, Percent_Identity=27.9569892473118, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=270, Percent_Identity=24.8148148148148, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=266, Percent_Identity=25.9398496240602, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17544670, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17564282, Length=188, Percent_Identity=28.1914893617021, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=261, Percent_Identity=26.8199233716475, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17560150, Length=264, Percent_Identity=25.3787878787879, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=195, Percent_Identity=26.1538461538462, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24644339, Length=261, Percent_Identity=29.5019157088123, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI23397609, Length=260, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357041, Length=253, Percent_Identity=31.2252964426877, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355319, Length=262, Percent_Identity=28.2442748091603, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571526, Length=253, Percent_Identity=30.0395256916996, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24643142, Length=259, Percent_Identity=27.4131274131274, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24639444, Length=263, Percent_Identity=27.3764258555133, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641232, Length=206, Percent_Identity=32.0388349514563, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281364892, Length=175, Percent_Identity=32, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859837, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116007234, Length=197, Percent_Identity=32.994923857868, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI161076371, Length=199, Percent_Identity=27.6381909547739, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27521; Mature: 27521

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLDLTGRKALVTGSTGGIGYAIAKELCDLGAEVGINGRTPERVEAALAKLRGEARAGRA
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCE
FAAPGDVATADGVSALVQALPAVDILVNNTGIFEPKPFFEIPDADWQRFFDVNVMSGVRL
ECCCCCCCCHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCH
SRAYTPGMAERGWGRVIFISSESGINTPTEMVHYGMTKTAQLAISRGLAQTVAGSGVTVN
HHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
SVLPGPTLSEGVAEFMKQMAGGAADADLEAMGRKFVAEHRPSSLIQRLARVEEVANLVAY
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LCSPAASATTGAALRVDGGVLQGLA
HHCCCCCCCCCCEEEECCHHHCCCC
>Mature Secondary Structure
MDLDLTGRKALVTGSTGGIGYAIAKELCDLGAEVGINGRTPERVEAALAKLRGEARAGRA
CCCCCCCCEEEEECCCCCHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCE
FAAPGDVATADGVSALVQALPAVDILVNNTGIFEPKPFFEIPDADWQRFFDVNVMSGVRL
ECCCCCCCCHHHHHHHHHHHCHHEEEECCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCCH
SRAYTPGMAERGWGRVIFISSESGINTPTEMVHYGMTKTAQLAISRGLAQTVAGSGVTVN
HHCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEE
SVLPGPTLSEGVAEFMKQMAGGAADADLEAMGRKFVAEHRPSSLIQRLARVEEVANLVAY
CCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LCSPAASATTGAALRVDGGVLQGLA
HHCCCCCCCCCCEEEECCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]