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The map label for this gene is cusS [H]

Identifier: 170749883

GI number: 170749883

Start: 3694668

End: 3696176

Strand: Reverse

Name: cusS [H]

Synonym: Mrad2831_3483

Alternate gene names: 170749883

Gene position: 3696176-3694668 (Counterclockwise)

Preceding gene: 170749884

Following gene: 170749882

Centisome position: 60.81

GC content: 72.03

Gene sequence:

>1509_bases
GTGAGCGACGTCGGGACCGAGGCGCCGGTCGCCCGCGAGACCGCGGGGGCGCGGTTCCAGAAGCTGTTCCGCACCACCGC
GTTCAAGCTGTCGCTGGCCTACCTGGCGATCTTCGCCCTCTGCGCGTTCCTGGCGCTCGGCTACGTCGCGTGGAACGCCC
GCGCGGTGCTCGACGACCAGATCGTCTCGACGATCGACGCAGAGATCAACGGCCTGTCGGAGCAGTACAATGCCGGCGGC
CTGCGGCGGCTGATCTCCGTGGTGGAGCGGCGCTCGCGGGAGCCCGGCGCGTCCCTCTACCTGGTGACCACGGCGGGCGG
CGACCATGTCGTCGGCAACGTCGGCTACGTGCCCGCCGCCGTGCTGGCGACGCCCGGCCAGAGCGAGACGCGCTACGGCC
GCAACGACGCGGATTCCGAGGCGCATCAGGCGATCGTGCGGGTCTTCACGCTGCCGGGCGGGTTCCGCCTGCTCGTCGGG
CGCGACACCGAGGAGCGCGACCGCCTGCGGGCGGTGATCGGACGCGCCTTCGTCACGTCGCTGGCCGCGGTCGTGCTCCT
CGGGGTGATCGGCGGCTGGCTCGCGGCGAGCCGTGTCCTGCGCCGGGTCGATGCCATGACACAGACCACGCGGGCGATCA
TGGCGGGCGACCTCGACGGCCGGCTCCACGTGGCCGGGAACGGCGACGAACTCGACCGCCTCGCGTCCAGCCTCAACCAG
ATGCTGGAGCGGATCGGCGAACTGATGCGCGGCATGCGGGAGGTCTCCGACAACATCGCGCATGACCTGAAGACGCCGCT
GACCCGCCTGCGCAACCGCGCCGACGAGGCCCTGCGCCGGGCCTCGACGCAGGAGGAACTGCGCGCGGCGCTGGAAGCCG
TGATCGACGAGGGCGACGGGCTGATCCGGGTGTTCAACGCGCTCCTGATGATCGCGCGGCTCGAGGCCGGAAGCGCCCGG
GAGATCCTCGTCCCGCTGGATATCGGACAAGCGGTGCGGGGCGTCGGCGAATTGTACGAGGCGCTCGCCGAGGACCAGGG
CCTGACCCTCACGGTGACCGCGCAGGACGACCTGATCATCGCCGGCAACCGCGAACTCATCGGTCAGGCCCTGGCCAACC
TGATCGACAACGCCCTGAAGTACGGGGCCGTCTCCGCCGGAGCGGAAGCCCGCGTGACCCTGGACGCGTGGCGCGAGGCG
GGCACGGTCCGACTCGCCGTCTCCGATCACGGCCCGGGCATTCCCGAGACGGACCGGGCCCGCGTGCTCGGACGCTTCGT
GCGCCTGGAGGATGCCCGGTCGCGACCCGGCTTCGGTCTGGGCCTCAGCCTCGTCAACGCCGTGGTGCGCCTGCATCACG
GGACCCTGCGGCTCGCCGACAACGGCCCCGGGCTGCGGGTCGAGATCGAGTTTCCGGCCTCGGCGCAGGCGCAGCCGGCG
GCCGGTGACATCCTGCCCGCTCCGCCATCGGGCGACCCTGTGCCGGACTCCCTGTCCCGCGCTACTTAG

Upstream 100 bases:

>100_bases
CGCGCCTGCGCAGCAAGCTCGACAAGGGCTTCCCGTCGCCGATCATCCACACGGTCCGGGGGGCCGGCTACGTGCTGCGC
TTCGACGACGGCGCGGCCGG

Downstream 100 bases:

>100_bases
GTCGCGACCTGTCCCACCGTCGCCCGGCCGTGACCGCGTCCGAGCGGCGGCGCGCCCGCGTTCCAGGAGGCCCCATGACC
CTCAAGACCAAGACCGCCCT

Product: integral membrane sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MSDVGTEAPVARETAGARFQKLFRTTAFKLSLAYLAIFALCAFLALGYVAWNARAVLDDQIVSTIDAEINGLSEQYNAGG
LRRLISVVERRSREPGASLYLVTTAGGDHVVGNVGYVPAAVLATPGQSETRYGRNDADSEAHQAIVRVFTLPGGFRLLVG
RDTEERDRLRAVIGRAFVTSLAAVVLLGVIGGWLAASRVLRRVDAMTQTTRAIMAGDLDGRLHVAGNGDELDRLASSLNQ
MLERIGELMRGMREVSDNIAHDLKTPLTRLRNRADEALRRASTQEELRAALEAVIDEGDGLIRVFNALLMIARLEAGSAR
EILVPLDIGQAVRGVGELYEALAEDQGLTLTVTAQDDLIIAGNRELIGQALANLIDNALKYGAVSAGAEARVTLDAWREA
GTVRLAVSDHGPGIPETDRARVLGRFVRLEDARSRPGFGLGLSLVNAVVRLHHGTLRLADNGPGLRVEIEFPASAQAQPA
AGDILPAPPSGDPVPDSLSRAT

Sequences:

>Translated_502_residues
MSDVGTEAPVARETAGARFQKLFRTTAFKLSLAYLAIFALCAFLALGYVAWNARAVLDDQIVSTIDAEINGLSEQYNAGG
LRRLISVVERRSREPGASLYLVTTAGGDHVVGNVGYVPAAVLATPGQSETRYGRNDADSEAHQAIVRVFTLPGGFRLLVG
RDTEERDRLRAVIGRAFVTSLAAVVLLGVIGGWLAASRVLRRVDAMTQTTRAIMAGDLDGRLHVAGNGDELDRLASSLNQ
MLERIGELMRGMREVSDNIAHDLKTPLTRLRNRADEALRRASTQEELRAALEAVIDEGDGLIRVFNALLMIARLEAGSAR
EILVPLDIGQAVRGVGELYEALAEDQGLTLTVTAQDDLIIAGNRELIGQALANLIDNALKYGAVSAGAEARVTLDAWREA
GTVRLAVSDHGPGIPETDRARVLGRFVRLEDARSRPGFGLGLSLVNAVVRLHHGTLRLADNGPGLRVEIEFPASAQAQPA
AGDILPAPPSGDPVPDSLSRAT
>Mature_501_residues
SDVGTEAPVARETAGARFQKLFRTTAFKLSLAYLAIFALCAFLALGYVAWNARAVLDDQIVSTIDAEINGLSEQYNAGGL
RRLISVVERRSREPGASLYLVTTAGGDHVVGNVGYVPAAVLATPGQSETRYGRNDADSEAHQAIVRVFTLPGGFRLLVGR
DTEERDRLRAVIGRAFVTSLAAVVLLGVIGGWLAASRVLRRVDAMTQTTRAIMAGDLDGRLHVAGNGDELDRLASSLNQM
LERIGELMRGMREVSDNIAHDLKTPLTRLRNRADEALRRASTQEELRAALEAVIDEGDGLIRVFNALLMIARLEAGSARE
ILVPLDIGQAVRGVGELYEALAEDQGLTLTVTAQDDLIIAGNRELIGQALANLIDNALKYGAVSAGAEARVTLDAWREAG
TVRLAVSDHGPGIPETDRARVLGRFVRLEDARSRPGFGLGLSLVNAVVRLHHGTLRLADNGPGLRVEIEFPASAQAQPAA
GDILPAPPSGDPVPDSLSRAT

Specific function: Member of the two-component regulatory system CusS/CusR. Copper ion sensor. Could also be a silver ion sensor. Probably activates CusR by phosphorylation [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=316, Percent_Identity=30.379746835443, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788393, Length=277, Percent_Identity=27.7978339350181, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788279, Length=280, Percent_Identity=23.9285714285714, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1787894, Length=244, Percent_Identity=31.9672131147541, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790346, Length=248, Percent_Identity=29.0322580645161, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790861, Length=302, Percent_Identity=28.8079470198675, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=210, Percent_Identity=30, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=27.3127753303965, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790551, Length=235, Percent_Identity=28.936170212766, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1789149, Length=314, Percent_Identity=25.4777070063694, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=300, Percent_Identity=26.3333333333333, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789403, Length=307, Percent_Identity=27.3615635179153, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787374, Length=290, Percent_Identity=24.1379310344828, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 53617; Mature: 53486

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVGTEAPVARETAGARFQKLFRTTAFKLSLAYLAIFALCAFLALGYVAWNARAVLDDQ
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IVSTIDAEINGLSEQYNAGGLRRLISVVERRSREPGASLYLVTTAGGDHVVGNVGYVPAA
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEECCCCCCCHH
VLATPGQSETRYGRNDADSEAHQAIVRVFTLPGGFRLLVGRDTEERDRLRAVIGRAFVTS
HEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LAAVVLLGVIGGWLAASRVLRRVDAMTQTTRAIMAGDLDGRLHVAGNGDELDRLASSLNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
MLERIGELMRGMREVSDNIAHDLKTPLTRLRNRADEALRRASTQEELRAALEAVIDEGDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LIRVFNALLMIARLEAGSAREILVPLDIGQAVRGVGELYEALAEDQGLTLTVTAQDDLII
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
AGNRELIGQALANLIDNALKYGAVSAGAEARVTLDAWREAGTVRLAVSDHGPGIPETDRA
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEEEECCCCCCCCCHHH
RVLGRFVRLEDARSRPGFGLGLSLVNAVVRLHHGTLRLADNGPGLRVEIEFPASAQAQPA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCCCCCCC
AGDILPAPPSGDPVPDSLSRAT
CCCCCCCCCCCCCCCCHHCCCC
>Mature Secondary Structure 
SDVGTEAPVARETAGARFQKLFRTTAFKLSLAYLAIFALCAFLALGYVAWNARAVLDDQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
IVSTIDAEINGLSEQYNAGGLRRLISVVERRSREPGASLYLVTTAGGDHVVGNVGYVPAA
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEECCCCCCCHH
VLATPGQSETRYGRNDADSEAHQAIVRVFTLPGGFRLLVGRDTEERDRLRAVIGRAFVTS
HEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHH
LAAVVLLGVIGGWLAASRVLRRVDAMTQTTRAIMAGDLDGRLHVAGNGDELDRLASSLNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHH
MLERIGELMRGMREVSDNIAHDLKTPLTRLRNRADEALRRASTQEELRAALEAVIDEGDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
LIRVFNALLMIARLEAGSAREILVPLDIGQAVRGVGELYEALAEDQGLTLTVTAQDDLII
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEE
AGNRELIGQALANLIDNALKYGAVSAGAEARVTLDAWREAGTVRLAVSDHGPGIPETDRA
ECCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHCCCEEEEEEECCCCCCCCCHHH
RVLGRFVRLEDARSRPGFGLGLSLVNAVVRLHHGTLRLADNGPGLRVEIEFPASAQAQPA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEECCCCCCCCCC
AGDILPAPPSGDPVPDSLSRAT
CCCCCCCCCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]