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The map label for this gene is yxeN [H]

Identifier: 170749710

GI number: 170749710

Start: 3510438

End: 3511187

Strand: Reverse

Name: yxeN [H]

Synonym: Mrad2831_3309

Alternate gene names: 170749710

Gene position: 3511187-3510438 (Counterclockwise)

Preceding gene: 170749711

Following gene: 170749709

Centisome position: 57.77

GC content: 66.53

Gene sequence:

>750_bases
ATGATCGCCACGATCCAGGCGTGGTTCCAGCAGCTCTACGAGACCACGGGCTTCAACTTCACGGTCTTCTACGATCCCTA
CGACTGGGACCGGTACGTGGCCGGCCTGCGGATGACGCTGCTCCTCGGGATCAGCACCATCCTGGCCAGCCTCGTCATCG
GCGCCGTGGGCGCGCTCCTGCAGAGCGGGCCGTCGCGTGTCCTGCGGGCGCTGGTCAACGGCTTCGTGGTGGTGTTCCGC
AACACGCCGCCGCTCGTGCAGATCTTCTTCTTCTACTTCGGCCTCGGGACGCTGCTCCCGGACATCCGCTCGGCCGACGG
CCTGCGGGCGCCGATCCTCAACAATGTCCAGTGGGCGATCATCTCGCTGTCGCTGTTCGCGGGCGCGTTCAACGTCGAGA
TCTTCCGCTCGGGCATCGAGGCCGTGCCGCGGACCACGGTCGAGGCCGCCGAGGCCCTGGGCTACACCCGCTTCAAGGCC
TACCGGTACGTCGTCCTGCCGCTGGCCCTGCGCGTCTGCCTGCCGGCCCTGAACAACAACCTGATCAACCTCCTGAAGAC
CACGACACTGGCCTACGCCATCGCGGTCCCGGAGACCCTGTACGCGGTCAAGCAGATCTGGTCCGAGTCGCAGAACGTGC
TGGAGATGATGCTGGTGCTGCTGGCGACTTACGCGGTCCTCGTCGGCGTGCTCGTCTGGATCATGCACCGCTGGGAGCGC
GCCCTGCGCATCCCGGGATACGGCCGATGA

Upstream 100 bases:

>100_bases
GGCATCGAGCCGACCGCTTACTCGAAGGCGATGCACGAGAAGTACAAGAACGCGACCAACTGATGCGCGCCTGCACGACG
GCCTTGAGCGTTCGCCCGCG

Downstream 100 bases:

>100_bases
AAGCGGCCGATCCGCGGGGCGGGCGGCCGAATGCCCTGCCGGTCCTCCTGCCCTTCGCGCTCCCGGAGGACCGCGCGGCG
GCGCGCCTGCGCCCGGCCCA

Product: polar amino acid ABC transporter inner membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MIATIQAWFQQLYETTGFNFTVFYDPYDWDRYVAGLRMTLLLGISTILASLVIGAVGALLQSGPSRVLRALVNGFVVVFR
NTPPLVQIFFFYFGLGTLLPDIRSADGLRAPILNNVQWAIISLSLFAGAFNVEIFRSGIEAVPRTTVEAAEALGYTRFKA
YRYVVLPLALRVCLPALNNNLINLLKTTTLAYAIAVPETLYAVKQIWSESQNVLEMMLVLLATYAVLVGVLVWIMHRWER
ALRIPGYGR

Sequences:

>Translated_249_residues
MIATIQAWFQQLYETTGFNFTVFYDPYDWDRYVAGLRMTLLLGISTILASLVIGAVGALLQSGPSRVLRALVNGFVVVFR
NTPPLVQIFFFYFGLGTLLPDIRSADGLRAPILNNVQWAIISLSLFAGAFNVEIFRSGIEAVPRTTVEAAEALGYTRFKA
YRYVVLPLALRVCLPALNNNLINLLKTTTLAYAIAVPETLYAVKQIWSESQNVLEMMLVLLATYAVLVGVLVWIMHRWER
ALRIPGYGR
>Mature_249_residues
MIATIQAWFQQLYETTGFNFTVFYDPYDWDRYVAGLRMTLLLGISTILASLVIGAVGALLQSGPSRVLRALVNGFVVVFR
NTPPLVQIFFFYFGLGTLLPDIRSADGLRAPILNNVQWAIISLSLFAGAFNVEIFRSGIEAVPRTTVEAAEALGYTRFKA
YRYVVLPLALRVCLPALNNNLINLLKTTTLAYAIAVPETLYAVKQIWSESQNVLEMMLVLLATYAVLVGVLVWIMHRWER
ALRIPGYGR

Specific function: Probably part of the ABC transporter complex yxeMNO that could be involved in amino-acid import. May transport S- methylcysteine. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786874, Length=216, Percent_Identity=29.6296296296296, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788645, Length=244, Percent_Identity=29.9180327868852, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI1788226, Length=210, Percent_Identity=31.9047619047619, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787030, Length=225, Percent_Identity=29.3333333333333, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788646, Length=213, Percent_Identity=27.6995305164319, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1787086, Length=182, Percent_Identity=27.4725274725275, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI48994930, Length=126, Percent_Identity=31.7460317460317, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786873, Length=201, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787087, Length=222, Percent_Identity=26.1261261261261, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082239, Length=175, Percent_Identity=29.1428571428571, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 27858; Mature: 27858

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATIQAWFQQLYETTGFNFTVFYDPYDWDRYVAGLRMTLLLGISTILASLVIGAVGALL
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGPSRVLRALVNGFVVVFRNTPPLVQIFFFYFGLGTLLPDIRSADGLRAPILNNVQWAI
HCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHH
ISLSLFAGAFNVEIFRSGIEAVPRTTVEAAEALGYTRFKAYRYVVLPLALRVCLPALNNN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LINLLKTTTLAYAIAVPETLYAVKQIWSESQNVLEMMLVLLATYAVLVGVLVWIMHRWER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRIPGYGR
HHCCCCCCC
>Mature Secondary Structure
MIATIQAWFQQLYETTGFNFTVFYDPYDWDRYVAGLRMTLLLGISTILASLVIGAVGALL
CCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGPSRVLRALVNGFVVVFRNTPPLVQIFFFYFGLGTLLPDIRSADGLRAPILNNVQWAI
HCCHHHHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHH
ISLSLFAGAFNVEIFRSGIEAVPRTTVEAAEALGYTRFKAYRYVVLPLALRVCLPALNNN
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LINLLKTTTLAYAIAVPETLYAVKQIWSESQNVLEMMLVLLATYAVLVGVLVWIMHRWER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRIPGYGR
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]