Click here to switch to the map view.

The map label for this gene is livK [H]

Identifier: 170749704

GI number: 170749704

Start: 3503338

End: 3504519

Strand: Direct

Name: livK [H]

Synonym: Mrad2831_3303

Alternate gene names: 170749704

Gene position: 3503338-3504519 (Clockwise)

Preceding gene: 170749702

Following gene: 170749705

Centisome position: 57.64

GC content: 70.22

Gene sequence:

>1182_bases
ATGGCCTCCTTCCTGCCCCCCGCCCTGCGCCGCATCGCGGTGACGACGAGCGCCCTGCTCGGCCTCGCCGTCTCGGCCGC
GCAGGCGGCCGACACCGTCAAGATCGGCCTCGTGACCGCCCTGAGCGGCCAGTCCGCGAAATCCGGCGAGGCGATCTCCC
GCGGGATCGGGCTCGCGATCGACGAGATCAACGCCAAGGGCGGCGTCCTCGGGCGCCCCCTCGAGCTCGTGGCCCGCGAC
GACGAGGCCAACCCGTCCAAGGGCGTGCTGGCGACCCGCGAGCTGATCCAGCGCGTCGGCGTCGTCGCGGTGGTCGGCGG
CCTCGACACGCCGGTCTCGATGGCGATCGTGCCGATCGCCAACCAGATGAAGGTGCCGTTCATCGGACCCTGGGCGGCCG
GCACAGGCATCACCCGCAACGGCGCCGCCGACAATTACGCCTTCCGCGTCTCCGCGGTCGACGCCCTCGTCGACGAGGCG
CTGGTCGCCTACGCGGTGAAGACCTACGGGGCGAAGAAGCCCGGCATGATCCTCGTCAACAACGCCTGGGGCGAGTCGAA
CCAGAAGGGCCTCAAGGACGCCCTCCAGGCGGCCAACCTCCCGGCGGCCGGCATCGAGAAGTACGAGGCCAACGACGTCG
ACATGGTGCCCCAGCTGTCCCGGCTGAAGGAGGCCGGCGCCGACGCGCTGTTCCTCGTCGGCAATGTCGGACCCTCGGCC
CAGGTGGTGAAGTCGCTGGACCGGATGGGCTGGACGGTGCCGGTCGTCTCGCACTGGGGCCCGGCCGGCGGGCGCTTCGA
CGAGCTCGCCGGCCCGAGCGCCGGCAAGGTCCACTTCATCCAGACCTTCACCTTCGCGGGCAACGCGTCGCCGAAGGCGA
GCGCGGTGCTCGATGCGCTCAAGGCCAAGTACCCGGCGATCAAGTCGATGGCCGACGTCACCCCCGCCGTCGGCATCGCC
AACGCCTACGACGCGACGCACCTGCTGGCGCTCGCCATCCAGGCCGCCGGCTCGACCGAGGGCCCGAAGGTCCGCGACGG
CTTCTACAAGATCGGCACCTATCCGGGCCTGATCAAAACCTACGACAAGCCCTTCGCCCCCGGCCAGCACGACGCGCTGG
GACCGAACGATTACGTGTTCACCCGCTTCAAGGACGGTGAGATCGTCGCGGTGACCAACTGA

Upstream 100 bases:

>100_bases
CCGGGAGTGCACGCACGGTCAGGCGCGGTCGAAGAAGCATCGGGTTTTCAGTGGCATGGAGGTTGCGGTCGGCTGGTCTG
CCCCGCTCCCGGAGACAGCC

Downstream 100 bases:

>100_bases
GCCCGGCGATGCTGACCTCCATCCTCGTCAGCGGCCTCGGCCTCGGCAGCATGTACGGGCTCGTGGCCCTCGGCTTCCAC
GTGACCTACGCGGTGTCGGG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MASFLPPALRRIAVTTSALLGLAVSAAQAADTVKIGLVTALSGQSAKSGEAISRGIGLAIDEINAKGGVLGRPLELVARD
DEANPSKGVLATRELIQRVGVVAVVGGLDTPVSMAIVPIANQMKVPFIGPWAAGTGITRNGAADNYAFRVSAVDALVDEA
LVAYAVKTYGAKKPGMILVNNAWGESNQKGLKDALQAANLPAAGIEKYEANDVDMVPQLSRLKEAGADALFLVGNVGPSA
QVVKSLDRMGWTVPVVSHWGPAGGRFDELAGPSAGKVHFIQTFTFAGNASPKASAVLDALKAKYPAIKSMADVTPAVGIA
NAYDATHLLALAIQAAGSTEGPKVRDGFYKIGTYPGLIKTYDKPFAPGQHDALGPNDYVFTRFKDGEIVAVTN

Sequences:

>Translated_393_residues
MASFLPPALRRIAVTTSALLGLAVSAAQAADTVKIGLVTALSGQSAKSGEAISRGIGLAIDEINAKGGVLGRPLELVARD
DEANPSKGVLATRELIQRVGVVAVVGGLDTPVSMAIVPIANQMKVPFIGPWAAGTGITRNGAADNYAFRVSAVDALVDEA
LVAYAVKTYGAKKPGMILVNNAWGESNQKGLKDALQAANLPAAGIEKYEANDVDMVPQLSRLKEAGADALFLVGNVGPSA
QVVKSLDRMGWTVPVVSHWGPAGGRFDELAGPSAGKVHFIQTFTFAGNASPKASAVLDALKAKYPAIKSMADVTPAVGIA
NAYDATHLLALAIQAAGSTEGPKVRDGFYKIGTYPGLIKTYDKPFAPGQHDALGPNDYVFTRFKDGEIVAVTN
>Mature_392_residues
ASFLPPALRRIAVTTSALLGLAVSAAQAADTVKIGLVTALSGQSAKSGEAISRGIGLAIDEINAKGGVLGRPLELVARDD
EANPSKGVLATRELIQRVGVVAVVGGLDTPVSMAIVPIANQMKVPFIGPWAAGTGITRNGAADNYAFRVSAVDALVDEAL
VAYAVKTYGAKKPGMILVNNAWGESNQKGLKDALQAANLPAAGIEKYEANDVDMVPQLSRLKEAGADALFLVGNVGPSAQ
VVKSLDRMGWTVPVVSHWGPAGGRFDELAGPSAGKVHFIQTFTFAGNASPKASAVLDALKAKYPAIKSMADVTPAVGIAN
AYDATHLLALAIQAAGSTEGPKVRDGFYKIGTYPGLIKTYDKPFAPGQHDALGPNDYVFTRFKDGEIVAVTN

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=335, Percent_Identity=25.9701492537313, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994941, Length=285, Percent_Identity=22.8070175438596, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40643; Mature: 40512

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFLPPALRRIAVTTSALLGLAVSAAQAADTVKIGLVTALSGQSAKSGEAISRGIGLAI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCHHHHHCCCEEE
DEINAKGGVLGRPLELVARDDEANPSKGVLATRELIQRVGVVAVVGGLDTPVSMAIVPIA
EECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEECC
NQMKVPFIGPWAAGTGITRNGAADNYAFRVSAVDALVDEALVAYAVKTYGAKKPGMILVN
CCCCCCEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NAWGESNQKGLKDALQAANLPAAGIEKYEANDVDMVPQLSRLKEAGADALFLVGNVGPSA
CCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
QVVKSLDRMGWTVPVVSHWGPAGGRFDELAGPSAGKVHFIQTFTFAGNASPKASAVLDAL
HHHHHHHHCCCEEEEHHCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
KAKYPAIKSMADVTPAVGIANAYDATHLLALAIQAAGSTEGPKVRDGFYKIGTYPGLIKT
HHHCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHH
YDKPFAPGQHDALGPNDYVFTRFKDGEIVAVTN
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEC
>Mature Secondary Structure 
ASFLPPALRRIAVTTSALLGLAVSAAQAADTVKIGLVTALSGQSAKSGEAISRGIGLAI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEEECCCCCCCCHHHHHCCCEEE
DEINAKGGVLGRPLELVARDDEANPSKGVLATRELIQRVGVVAVVGGLDTPVSMAIVPIA
EECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEECC
NQMKVPFIGPWAAGTGITRNGAADNYAFRVSAVDALVDEALVAYAVKTYGAKKPGMILVN
CCCCCCEECCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NAWGESNQKGLKDALQAANLPAAGIEKYEANDVDMVPQLSRLKEAGADALFLVGNVGPSA
CCCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHH
QVVKSLDRMGWTVPVVSHWGPAGGRFDELAGPSAGKVHFIQTFTFAGNASPKASAVLDAL
HHHHHHHHCCCEEEEHHCCCCCCCCCHHHCCCCCCCEEEEEEEEECCCCCCHHHHHHHHH
KAKYPAIKSMADVTPAVGIANAYDATHLLALAIQAAGSTEGPKVRDGFYKIGTYPGLIKT
HHHCHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHHHH
YDKPFAPGQHDALGPNDYVFTRFKDGEIVAVTN
CCCCCCCCCCCCCCCCCEEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]