BacMap

Definition	Methylobacterium radiotolerans JCM 2831 chromosome, complete genome.
Accession	NC_010505
Length	6,077,833

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The map label for this gene is paaF [C]

Identifier: 170749666

GI number: 170749666

Start: 3457275

End: 3458078

Strand: Direct

Name: paaF [C]

Synonym: Mrad2831_3263

Alternate gene names: 170749666

Gene position: 3457275-3458078 (Clockwise)

Preceding gene: 170749665

Following gene: 170749667

Centisome position: 56.88

GC content: 72.01

Gene sequence:

>804_bases
ATGAGTGCCGAGACTGGGACCCGCGACGCGGGCGAGGACCTGCTGTTCTCCGTCGCCGAGGGGGTCGCGACCATTACGCT
GAACCGGCCCGAGCGGAAGAACGCCTTCACCTTCCCGATGATCGAGGCCTGGACGGCGGCCCTGCAGCGCTGCCGCACCG
ACGCGGCGATCCGCGTCGTGGTGGTGACCGGCGCGGGCTCGGCCTTCTGCAGCGGCGGCGACATCGTCGAGATGGGGGAG
CGCCTCGACCAGCCGCCGGCCCGGCGCAAGGAGGAGCTCCACGGGCGGATCCAGCGCATCCCGCTGACGCTGGAGGATCT
CGACAAGCCGGTGATCGCCGCCCTGAACGGCGTGGCCACCGGCGCGGGCCTCGACCTCGCCCTGATGTGCGACCTGCGCT
ACGCCGCGCAGAGCGCCCGCTTCGCCGAGACCTACGTGCGGGTCGGCTTGGTCCCGGGCGCGGGCGGCGCCCATTTCCTG
CCCCGGCTCGTCGGGACCGCGAAGGCGCTGGAGCTGTTCTTCACCGGCGATTTCGTCGACGCCGACGAGGCCCTGCGGAT
CGGCCTCGTCAACGGCGTCCACGCCGACGCCGACCTGATGCCGGCCGTCGCGGCGCTGGCGCGGAAGATCGCCCAGGCGC
CGCCGCTGACCCTGAGCCTGATCAAGCGCGCGGTCTATCAGGGCATGCGCAACGACCTGCGCACCAACCTCGACCTGATC
TCCTCGCACTACGCCGTCGTCACCGCGACGCCCGAGCATCGGGAGGCGGTGCAGGCCTTTATCGGCCAGCGCGACAGGGG
CTGA

Upstream 100 bases:

>100_bases
CCGCCGAGGTCCTAGCCGAGGCCGGGCTCGACCCGGCCGAGATCGCGGCCCTGATCGACGCGCCGGGCGAAACCCACCCC
GCGCCCCTGGAGGCCGCCCG

Downstream 100 bases:

>100_bases
ACCACCGGCGCGAACAACATCTCCCCCGGGCATCACGCTTTGGTGCTTGTCGCCACCGCGACTACCTGCAAGTCTATCCA
AAATTCAATCCGCGCAACGA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MSAETGTRDAGEDLLFSVAEGVATITLNRPERKNAFTFPMIEAWTAALQRCRTDAAIRVVVVTGAGSAFCSGGDIVEMGE
RLDQPPARRKEELHGRIQRIPLTLEDLDKPVIAALNGVATGAGLDLALMCDLRYAAQSARFAETYVRVGLVPGAGGAHFL
PRLVGTAKALELFFTGDFVDADEALRIGLVNGVHADADLMPAVAALARKIAQAPPLTLSLIKRAVYQGMRNDLRTNLDLI
SSHYAVVTATPEHREAVQAFIGQRDRG

Sequences:

>Translated_267_residues
MSAETGTRDAGEDLLFSVAEGVATITLNRPERKNAFTFPMIEAWTAALQRCRTDAAIRVVVVTGAGSAFCSGGDIVEMGE
RLDQPPARRKEELHGRIQRIPLTLEDLDKPVIAALNGVATGAGLDLALMCDLRYAAQSARFAETYVRVGLVPGAGGAHFL
PRLVGTAKALELFFTGDFVDADEALRIGLVNGVHADADLMPAVAALARKIAQAPPLTLSLIKRAVYQGMRNDLRTNLDLI
SSHYAVVTATPEHREAVQAFIGQRDRG
>Mature_266_residues
SAETGTRDAGEDLLFSVAEGVATITLNRPERKNAFTFPMIEAWTAALQRCRTDAAIRVVVVTGAGSAFCSGGDIVEMGER
LDQPPARRKEELHGRIQRIPLTLEDLDKPVIAALNGVATGAGLDLALMCDLRYAAQSARFAETYVRVGLVPGAGGAHFLP
RLVGTAKALELFFTGDFVDADEALRIGLVNGVHADADLMPAVAALARKIAQAPPLTLSLIKRAVYQGMRNDLRTNLDLIS
SHYAVVTATPEHREAVQAFIGQRDRG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=34.156378600823, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI70995211, Length=252, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI31542718, Length=248, Percent_Identity=30.241935483871, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=30.8300395256917, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI45643119, Length=225, Percent_Identity=28.8888888888889, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260275230, Length=225, Percent_Identity=28.8888888888889, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=28.8888888888889, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI68989263, Length=203, Percent_Identity=32.0197044334975, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI37594471, Length=170, Percent_Identity=33.5294117647059, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI37594469, Length=170, Percent_Identity=33.5294117647059, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI221136756, Length=222, Percent_Identity=26.1261261261261, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI221136753, Length=222, Percent_Identity=26.1261261261261, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI221307494, Length=222, Percent_Identity=26.1261261261261, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI25453481, Length=222, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI51538573, Length=222, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI4757966, Length=222, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI51538581, Length=222, Percent_Identity=27.027027027027, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI20127408, Length=180, Percent_Identity=30, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI116235487, Length=214, Percent_Identity=28.5046728971963, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4757968, Length=222, Percent_Identity=26.5765765765766, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI49355787, Length=222, Percent_Identity=26.5765765765766, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI157694516, Length=249, Percent_Identity=24.0963855421687, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI213417737, Length=249, Percent_Identity=24.0963855421687, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI261878539, Length=97, Percent_Identity=38.1443298969072, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=253, Percent_Identity=33.201581027668, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=37.7431906614786, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788597, Length=217, Percent_Identity=36.405529953917, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI221142681, Length=262, Percent_Identity=32.4427480916031, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788682, Length=198, Percent_Identity=32.3232323232323, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790281, Length=190, Percent_Identity=30.5263157894737, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082183, Length=254, Percent_Identity=25.5905511811024, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=34.9593495934959, Blast_Score=141, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554946, Length=245, Percent_Identity=33.0612244897959, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17540714, Length=229, Percent_Identity=33.6244541484716, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560910, Length=210, Percent_Identity=29.5238095238095, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17536985, Length=238, Percent_Identity=29.4117647058824, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=234, Percent_Identity=27.7777777777778, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=247, Percent_Identity=25.1012145748988, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558304, Length=176, Percent_Identity=30.1136363636364, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=176, Percent_Identity=32.9545454545455, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=176, Percent_Identity=32.9545454545455, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=176, Percent_Identity=32.9545454545455, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144160, Length=162, Percent_Identity=26.5432098765432, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI25144157, Length=162, Percent_Identity=26.5432098765432, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=207, Percent_Identity=28.0193236714976, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=232, Percent_Identity=25, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=226, Percent_Identity=23.8938053097345, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20129971, Length=246, Percent_Identity=33.739837398374, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24653477, Length=246, Percent_Identity=33.739837398374, Blast_Score=140, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=34, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21357171, Length=220, Percent_Identity=31.3636363636364, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24654903, Length=240, Percent_Identity=24.5833333333333, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=28.2352941176471, Blast_Score=87, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=31.651376146789, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571729, Length=162, Percent_Identity=30.2469135802469, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI28571730, Length=162, Percent_Identity=30.2469135802469, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19922422, Length=226, Percent_Identity=24.7787610619469, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28604; Mature: 28473

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAETGTRDAGEDLLFSVAEGVATITLNRPERKNAFTFPMIEAWTAALQRCRTDAAIRVV
CCCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VVTGAGSAFCSGGDIVEMGERLDQPPARRKEELHGRIQRIPLTLEDLDKPVIAALNGVAT
EEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCC
GAGLDLALMCDLRYAAQSARFAETYVRVGLVPGAGGAHFLPRLVGTAKALELFFTGDFVD
CCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
ADEALRIGLVNGVHADADLMPAVAALARKIAQAPPLTLSLIKRAVYQGMRNDLRTNLDLI
CCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSHYAVVTATPEHREAVQAFIGQRDRG
HCCEEEEEECCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAETGTRDAGEDLLFSVAEGVATITLNRPERKNAFTFPMIEAWTAALQRCRTDAAIRVV
CCCCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
VVTGAGSAFCSGGDIVEMGERLDQPPARRKEELHGRIQRIPLTLEDLDKPVIAALNGVAT
EEECCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCC
GAGLDLALMCDLRYAAQSARFAETYVRVGLVPGAGGAHFLPRLVGTAKALELFFTGDFVD
CCCCCEEEEHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
ADEALRIGLVNGVHADADLMPAVAALARKIAQAPPLTLSLIKRAVYQGMRNDLRTNLDLI
CCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SSHYAVVTATPEHREAVQAFIGQRDRG
HCCEEEEEECCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA