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The map label for this gene is gsiB [H]

Identifier: 170749646

GI number: 170749646

Start: 3435796

End: 3437325

Strand: Reverse

Name: gsiB [H]

Synonym: Mrad2831_3243

Alternate gene names: 170749646

Gene position: 3437325-3435796 (Counterclockwise)

Preceding gene: 170749647

Following gene: 170749645

Centisome position: 56.56

GC content: 66.54

Gene sequence:

>1530_bases
ATGTTCGAGCGCTTCCTCCTCGCCGCCGGCCTGCTCGCCGTGGCGGCCGCGCCCGCCGCCGCGGCGGGCGACCCGATCCT
GGCGATCAGCGGCCAGCCCGAGACTCTCGACCCGTACAACACGAACACCACGCTCACGACCGCCGTCACCAAGTCGTTCT
ACGAGGGCCTCTTCGGCTTCGACAAGGACCTGAAGGTCCGCAACGTCCTGGCCGAGAGCTACGAGACCTCGCCCGACGGC
CTGGTCTACACGTTCAAGCTGCGCGAGGGCGTCAAATTCCACGACGGCACGACGTTCGACGCCGCTGCGGTGAAGGCCAA
TCTCGACCGCGTCCTCGACCCGGAGAACCACCTCGCGCGGTACAACCAGTTCAACCGCATCAAGACGATCGAGGTGCTCT
CGCCCACGAGCCTGCGCATCACCCTGAAGGAGCCCTTCGGGCCGTTCATCAACTCGCTGGCCCACGCCTCGGCGGCGATC
ATCTCGCCCACGGCCCTGAAGAAGTACGGCAAGGACATCGCCTTCCACCCGGTCGGCACCGGGCCGTTCACCTTCGTCGA
CTGGAAGCAGACCGACTACGTCAAGGGCGCGAAGTTTGCCGATTATTGGCAGGCCGGCTTCCCGAAGGTCGACACCATCA
CGTGGAAGCCGGTGCCCGAGAACGGCACACGCGCCGCCATGCTGCAGACCGGAGAGGCCGACTTCGCCTTCCCGCTGCCG
TACGAGCAGGCCAAGATGCTCGAATCCAACAAGAAGCTGAAGATCGAGGTCCACGCCTCGATCATCGAGCGGTATCTCAG
CATGAACGTGCTGCAGAAGCCGTTCGACGACCTGCGCGTCCGCCAGGCGATCAACTACGCGATCAACAAGGAGGCGTTGG
CCAAGGTCGCCTTCTCGGGCTACGCCTTCCCCGCCCAGGGCATCGTCCCGCAGGGCGTGCTCTACGCCCACAAGATGGAT
CCGTGGCCCTACGATCCGGCCAAGGCGCGCCAGCTCCTGAAGGAGGCCGGCTACGCGAACGGCTTCGAGGCGACCCTCTG
GAGCGCCTACACCACCACCACCGCCCAGAAGGCGATCCAGTTCCTCCAGCAGCAGCTCGGGCAGGTCGGCATCAAGGTCC
AGACCCTGGCCCTGGAGCCCGGCCAGCGCACCGAGTGGGTGCAGACCGCTCCGGATCCGAAGACCGCCCGCGTCCGCCTC
TACTATGCCGGCTGGTCGTCCTCGACCGGCGAGGCCGATTGGGGCCTGCGGCCGCTGCTGGCGACGGAGGCCTGGCCGCC
CAAGCTCAACAACACCGCCTATTACAGCAACGCCGAGTTCGACGCCCTCCTCGCCAGGGCGCTGGTCTCAACGAACGACG
CGGAGCGGGCCGAACTCTACGCCAAGGCCCAGGAGATCGTGATGAAGGACGCGCCCTGGGCGCCGCTGGTCACCGAGCAG
AACCTCTACGCGACCTCCGCGCGGCTTTCCGGCGTGTACGTCATGCCCGACGGCAACATCGATTCCGGCGAAATCGCCGT
CGCTCAGTAG

Upstream 100 bases:

>100_bases
GGCCCCGGCCATCTCGTCGCCCGCCACACCGTCGGCGGCCTCGCCGCCTGATATTGCCTATCCCCGCCAGCAGGATCGTC
CCATGCCCCGAGGTCTGACC

Downstream 100 bases:

>100_bases
CCGCCGATCGCGGCCCCTGCGCCGGGCGGCCCGATCGATGGCCGGCCCGGCGCGGCGCTCCCGCGCGATCGCCTCACGGA
AACCCCGGATCCCGGGCGCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MFERFLLAAGLLAVAAAPAAAAGDPILAISGQPETLDPYNTNTTLTTAVTKSFYEGLFGFDKDLKVRNVLAESYETSPDG
LVYTFKLREGVKFHDGTTFDAAAVKANLDRVLDPENHLARYNQFNRIKTIEVLSPTSLRITLKEPFGPFINSLAHASAAI
ISPTALKKYGKDIAFHPVGTGPFTFVDWKQTDYVKGAKFADYWQAGFPKVDTITWKPVPENGTRAAMLQTGEADFAFPLP
YEQAKMLESNKKLKIEVHASIIERYLSMNVLQKPFDDLRVRQAINYAINKEALAKVAFSGYAFPAQGIVPQGVLYAHKMD
PWPYDPAKARQLLKEAGYANGFEATLWSAYTTTTAQKAIQFLQQQLGQVGIKVQTLALEPGQRTEWVQTAPDPKTARVRL
YYAGWSSSTGEADWGLRPLLATEAWPPKLNNTAYYSNAEFDALLARALVSTNDAERAELYAKAQEIVMKDAPWAPLVTEQ
NLYATSARLSGVYVMPDGNIDSGEIAVAQ

Sequences:

>Translated_509_residues
MFERFLLAAGLLAVAAAPAAAAGDPILAISGQPETLDPYNTNTTLTTAVTKSFYEGLFGFDKDLKVRNVLAESYETSPDG
LVYTFKLREGVKFHDGTTFDAAAVKANLDRVLDPENHLARYNQFNRIKTIEVLSPTSLRITLKEPFGPFINSLAHASAAI
ISPTALKKYGKDIAFHPVGTGPFTFVDWKQTDYVKGAKFADYWQAGFPKVDTITWKPVPENGTRAAMLQTGEADFAFPLP
YEQAKMLESNKKLKIEVHASIIERYLSMNVLQKPFDDLRVRQAINYAINKEALAKVAFSGYAFPAQGIVPQGVLYAHKMD
PWPYDPAKARQLLKEAGYANGFEATLWSAYTTTTAQKAIQFLQQQLGQVGIKVQTLALEPGQRTEWVQTAPDPKTARVRL
YYAGWSSSTGEADWGLRPLLATEAWPPKLNNTAYYSNAEFDALLARALVSTNDAERAELYAKAQEIVMKDAPWAPLVTEQ
NLYATSARLSGVYVMPDGNIDSGEIAVAQ
>Mature_509_residues
MFERFLLAAGLLAVAAAPAAAAGDPILAISGQPETLDPYNTNTTLTTAVTKSFYEGLFGFDKDLKVRNVLAESYETSPDG
LVYTFKLREGVKFHDGTTFDAAAVKANLDRVLDPENHLARYNQFNRIKTIEVLSPTSLRITLKEPFGPFINSLAHASAAI
ISPTALKKYGKDIAFHPVGTGPFTFVDWKQTDYVKGAKFADYWQAGFPKVDTITWKPVPENGTRAAMLQTGEADFAFPLP
YEQAKMLESNKKLKIEVHASIIERYLSMNVLQKPFDDLRVRQAINYAINKEALAKVAFSGYAFPAQGIVPQGVLYAHKMD
PWPYDPAKARQLLKEAGYANGFEATLWSAYTTTTAQKAIQFLQQQLGQVGIKVQTLALEPGQRTEWVQTAPDPKTARVRL
YYAGWSSSTGEADWGLRPLLATEAWPPKLNNTAYYSNAEFDALLARALVSTNDAERAELYAKAQEIVMKDAPWAPLVTEQ
NLYATSARLSGVYVMPDGNIDSGEIAVAQ

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=493, Percent_Identity=58.0121703853955, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1789966, Length=523, Percent_Identity=28.6806883365201, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1787551, Length=538, Percent_Identity=26.9516728624535, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1789887, Length=345, Percent_Identity=30.7246376811594, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1787495, Length=533, Percent_Identity=24.577861163227, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1787762, Length=530, Percent_Identity=25.8490566037736, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1789397, Length=471, Percent_Identity=22.0806794055202, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI87081878, Length=476, Percent_Identity=20.7983193277311, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 56117; Mature: 56117

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFERFLLAAGLLAVAAAPAAAAGDPILAISGQPETLDPYNTNTTLTTAVTKSFYEGLFGF
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHCC
DKDLKVRNVLAESYETSPDGLVYTFKLREGVKFHDGTTFDAAAVKANLDRVLDPENHLAR
CCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHH
YNQFNRIKTIEVLSPTSLRITLKEPFGPFINSLAHASAAIISPTALKKYGKDIAFHPVGT
HHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCC
GPFTFVDWKQTDYVKGAKFADYWQAGFPKVDTITWKPVPENGTRAAMLQTGEADFAFPLP
CCEEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCEECCCC
YEQAKMLESNKKLKIEVHASIIERYLSMNVLQKPFDDLRVRQAINYAINKEALAKVAFSG
HHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHCC
YAFPAQGIVPQGVLYAHKMDPWPYDPAKARQLLKEAGYANGFEATLWSAYTTTTAQKAIQ
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FLQQQLGQVGIKVQTLALEPGQRTEWVQTAPDPKTARVRLYYAGWSSSTGEADWGLRPLL
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHH
ATEAWPPKLNNTAYYSNAEFDALLARALVSTNDAERAELYAKAQEIVMKDAPWAPLVTEQ
HCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC
NLYATSARLSGVYVMPDGNIDSGEIAVAQ
CCEEEHHHCCEEEEEECCCCCCCCEEEEC
>Mature Secondary Structure
MFERFLLAAGLLAVAAAPAAAAGDPILAISGQPETLDPYNTNTTLTTAVTKSFYEGLFGF
CCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCEEHHHHHHHHHHHHHCC
DKDLKVRNVLAESYETSPDGLVYTFKLREGVKFHDGTTFDAAAVKANLDRVLDPENHLAR
CCCHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCHHHHHH
YNQFNRIKTIEVLSPTSLRITLKEPFGPFINSLAHASAAIISPTALKKYGKDIAFHPVGT
HHHCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCC
GPFTFVDWKQTDYVKGAKFADYWQAGFPKVDTITWKPVPENGTRAAMLQTGEADFAFPLP
CCEEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCEECCCC
YEQAKMLESNKKLKIEVHASIIERYLSMNVLQKPFDDLRVRQAINYAINKEALAKVAFSG
HHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHCC
YAFPAQGIVPQGVLYAHKMDPWPYDPAKARQLLKEAGYANGFEATLWSAYTTTTAQKAIQ
CCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
FLQQQLGQVGIKVQTLALEPGQRTEWVQTAPDPKTARVRLYYAGWSSSTGEADWGLRPLL
HHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCHH
ATEAWPPKLNNTAYYSNAEFDALLARALVSTNDAERAELYAKAQEIVMKDAPWAPLVTEQ
HCCCCCCCCCCCEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEECC
NLYATSARLSGVYVMPDGNIDSGEIAVAQ
CCEEEHHHCCEEEEEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA