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The map label for this gene is ghrA [H]

Identifier: 170749626

GI number: 170749626

Start: 3411918

End: 3412850

Strand: Reverse

Name: ghrA [H]

Synonym: Mrad2831_3223

Alternate gene names: 170749626

Gene position: 3412850-3411918 (Counterclockwise)

Preceding gene: 170749627

Following gene: 170749624

Centisome position: 56.15

GC content: 74.92

Gene sequence:

>933_bases
GTGACCGAGACCCTGGTCTTCGCGAGCGCCGTCGACCCGATCGAGCCCTGGCGCGCGGCGCTGGAGGCGCTGCTGCCGGA
CGTGCGCGTCAGGCCGCCGGACGAGATCGGGCCCGGGGACGATGTCCGCTACGCGCTGGTCTGGAAGCCGCCGGCCGGCT
TCTTCGCGCGGCATCCCGGCCTGCGGCTCGTCACCATCCTGGGCGCGGGCGCCGACGCGCTCGTCGGCCGGGACGACCTG
CCCGACGTGCCGATCACCCGCCTGTCCGACCCCGAGATGGCGCGGATGATGACTCACTACGTGCTGTTCGCGGTGCTGCG
CTACGCCCGCGACATCCCGGCCTTCGAGGCGGCCCAGCGGGCCGGCCGCTGGCACTACGTCCACCCGCGCGAGGCCCGGG
AAATCCGGGTCGGCGTGCTGGGCCTCGGCGAGCTCGGCGGCGCGGCGGCGCTCGAACTTGCGCGGCAGGGGTTCTCCGTC
GCCGGCTGGTCGCGCACGCCGAAGGCGCTTCCCGGAGTCGACTGCCACGCGGGACCGGACGCGCTGATCCCGTTTCTCGC
GCGGAGCGAGATCCTGGTGGTGATGCTGCCGCTGACCCCTGAGACCCGGCGCCTGGTCGGAGCGGACGAGATCGCTGCGC
TGCCGCGGGGCGCCGCGCTCATCAACGTGTCGCGCGGGGCCGTGATCGACGAGGCGGCGCTCGTTGCGGCTCTGGCGGCC
GGCCATCTCGGTGGGGCGACGCTCGACGTGTTCGAGACCGAGCCGCTCGCGGCGGGACACCCGCTCTGGGGGATGGAGAG
CGTCCTCGTCACGCCGCACCTCGCCTCGGTGGCGATCCCCGCCTCCGCGGCCCGGCAGATCGCCGAGAACATCCACCGGG
TGCGCGCGGGCCGCGCGCCCGAGCACCGCGTGGACCTGACGCGGGGCTACTGA

Upstream 100 bases:

>100_bases
ACGTCTCGCGGGCGGCGACCGGCGTGGTCGCGGGGCTCGGCGGCCTCGGCTACGAGCTGGCCATCACCGCCCTCGCCACC
CTGGTGCGGGAGGCCCGCGC

Downstream 100 bases:

>100_bases
GAGGCTGGCACGGGCGATCTCACGGCCGACGCCTGTTTCCTGCGGCGCCTCCGGCCCGGGTCCGGCCCGTCAGGCCCGGG
TATAAGCGTCGTAGTTGCGC

Product: NAD-binding D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTETLVFASAVDPIEPWRAALEALLPDVRVRPPDEIGPGDDVRYALVWKPPAGFFARHPGLRLVTILGAGADALVGRDDL
PDVPITRLSDPEMARMMTHYVLFAVLRYARDIPAFEAAQRAGRWHYVHPREAREIRVGVLGLGELGGAAALELARQGFSV
AGWSRTPKALPGVDCHAGPDALIPFLARSEILVVMLPLTPETRRLVGADEIAALPRGAALINVSRGAVIDEAALVAALAA
GHLGGATLDVFETEPLAAGHPLWGMESVLVTPHLASVAIPASAARQIAENIHRVRAGRAPEHRVDLTRGY

Sequences:

>Translated_310_residues
MTETLVFASAVDPIEPWRAALEALLPDVRVRPPDEIGPGDDVRYALVWKPPAGFFARHPGLRLVTILGAGADALVGRDDL
PDVPITRLSDPEMARMMTHYVLFAVLRYARDIPAFEAAQRAGRWHYVHPREAREIRVGVLGLGELGGAAALELARQGFSV
AGWSRTPKALPGVDCHAGPDALIPFLARSEILVVMLPLTPETRRLVGADEIAALPRGAALINVSRGAVIDEAALVAALAA
GHLGGATLDVFETEPLAAGHPLWGMESVLVTPHLASVAIPASAARQIAENIHRVRAGRAPEHRVDLTRGY
>Mature_309_residues
TETLVFASAVDPIEPWRAALEALLPDVRVRPPDEIGPGDDVRYALVWKPPAGFFARHPGLRLVTILGAGADALVGRDDLP
DVPITRLSDPEMARMMTHYVLFAVLRYARDIPAFEAAQRAGRWHYVHPREAREIRVGVLGLGELGGAAALELARQGFSVA
GWSRTPKALPGVDCHAGPDALIPFLARSEILVVMLPLTPETRRLVGADEIAALPRGAALINVSRGAVIDEAALVAALAAG
HLGGATLDVFETEPLAAGHPLWGMESVLVTPHLASVAIPASAARQIAENIHRVRAGRAPEHRVDLTRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=143, Percent_Identity=34.965034965035, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI145580578, Length=267, Percent_Identity=26.5917602996255, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI4557499, Length=267, Percent_Identity=26.5917602996255, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI23308577, Length=176, Percent_Identity=30.6818181818182, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4557497, Length=267, Percent_Identity=26.2172284644195, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI61743967, Length=267, Percent_Identity=26.2172284644195, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI145580575, Length=263, Percent_Identity=26.9961977186312, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=35.2564102564103, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=31.1284046692607, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789279, Length=195, Percent_Identity=31.7948717948718, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17532191, Length=268, Percent_Identity=30.2238805970149, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI25147481, Length=192, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324055, Length=161, Percent_Identity=34.1614906832298, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320925, Length=195, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322116, Length=195, Percent_Identity=26.6666666666667, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28571528, Length=168, Percent_Identity=32.7380952380952, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28574286, Length=197, Percent_Identity=28.9340101522843, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585516, Length=161, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI45552429, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24585514, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28574282, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI45551003, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28574284, Length=164, Percent_Identity=28.6585365853659, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19921140, Length=197, Percent_Identity=27.9187817258883, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=25.1851851851852, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=25.1851851851852, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=25.1851851851852, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=25.1851851851852, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 33134; Mature: 33002

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETLVFASAVDPIEPWRAALEALLPDVRVRPPDEIGPGDDVRYALVWKPPAGFFARHPG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCCC
LRLVTILGAGADALVGRDDLPDVPITRLSDPEMARMMTHYVLFAVLRYARDIPAFEAAQR
CEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGRWHYVHPREAREIRVGVLGLGELGGAAALELARQGFSVAGWSRTPKALPGVDCHAGPD
CCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCH
ALIPFLARSEILVVMLPLTPETRRLVGADEIAALPRGAALINVSRGAVIDEAALVAALAA
HHHHHHCCCCEEEEEECCCCCHHHHCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
GHLGGATLDVFETEPLAAGHPLWGMESVLVTPHLASVAIPASAARQIAENIHRVRAGRAP
CCCCCCEEEEEECCCCCCCCCCCCCCHHEECCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
EHRVDLTRGY
CHHCCCCCCC
>Mature Secondary Structure 
TETLVFASAVDPIEPWRAALEALLPDVRVRPPDEIGPGDDVRYALVWKPPAGFFARHPG
CCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCHHHCCCC
LRLVTILGAGADALVGRDDLPDVPITRLSDPEMARMMTHYVLFAVLRYARDIPAFEAAQR
CEEEEEECCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AGRWHYVHPREAREIRVGVLGLGELGGAAALELARQGFSVAGWSRTPKALPGVDCHAGPD
CCCEEEECCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCH
ALIPFLARSEILVVMLPLTPETRRLVGADEIAALPRGAALINVSRGAVIDEAALVAALAA
HHHHHHCCCCEEEEEECCCCCHHHHCCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
GHLGGATLDVFETEPLAAGHPLWGMESVLVTPHLASVAIPASAARQIAENIHRVRAGRAP
CCCCCCEEEEEECCCCCCCCCCCCCCHHEECCCHHHHCCCHHHHHHHHHHHHHHHCCCCC
EHRVDLTRGY
CHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA