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The map label for this gene is dapE

Identifier: 170749554

GI number: 170749554

Start: 3329180

End: 3330358

Strand: Reverse

Name: dapE

Synonym: Mrad2831_3149

Alternate gene names: 170749554

Gene position: 3330358-3329180 (Counterclockwise)

Preceding gene: 170749555

Following gene: 170749549

Centisome position: 54.8

GC content: 71.59

Gene sequence:

>1179_bases
ATGCCGGCTGACCATCACGCCCCCGACGCGGCCCTGCGCCTCGCGCAGGACCTGATCCGCTGTCCGTCCGTGACACCGGA
AGATCGGGGCGCCCTCGACGTGGTCGCCGACGCGCTCCGGCCGGCCGGCTTCGCGATCGAGCGGCCGGTCTTCGCGGAGC
CCGGCTATCCCGATACGCCGAACCTCTACGCCCGCATCGGGCAGAACGGTCCGTGCCTCGTCTTTGCCGGCCACACCGAC
GTCGTGCCGGAGGGCGAGGGCGCCTGGCGCCACGATCCCTTCGCGGGCGCCGTGGCCGACGGGATGCTGTACGGCCGGGG
CGCGGCCGACATGAAGGGCGGCGTCGCCTGCATGCTCGCGGCGACCCTCGCCTTCCTCGACCGGCGCGGGGCCGATTTCG
GCGGCTCCATCGCGTTTCTGATCACCGGCGACGAGGAGGGACCCGCGGTCAACGGCACCGTGAAGCTCCTCGACTGGGCG
CGGCGCCGCGGCGAGCGATTCGACCATTGCGTGCTCGGGGAGCCGACCAACCCGGGCCGGCTCGGCGAGATGATCAAGAT
CGGCCGGCGCGGGTCGCTGACCGGCAAGCTCACCGTGCTCGGGCGGCAGGGCCACGTCGCCTACCCGCACAAGGCCGAAA
ACCCGATTCCCGGCCTGCTGCGCCTCGCCTCGGCGCTGATCGCCGAGCCTCTGGATCGTGGGACCGCGCATTTCGACGCC
TCGAACCTCGAATTCACCACGGTCGACGTCGGCAATCCAGCCACCAACGTGATCCCGTCGACCGCCCGGGCAACCTTCAA
CGTCCGCTTCAACGACGACTGGACGGCCGAGAGCCTGGGCGCCGAGATCCGCCGGCGCCTGGAGCAGGCGGCCGGGAACG
CGGTCCGCTTCACCCTCGACCTCCAGCCCTCGAACGCGCCGGCCTTCCTGACGCGGCCGGACGCCTTCGTGACGCTCGTC
GCCGAGGCGATCCGGGCCGAGACCGGTCTGACGCCGACGCTCTCCACCACCGGCGGCACGTCGGACGCGCGCTTCATCAA
GGATGCCTGCCCGGTGATCGAGTTCGGCCTCGTCGGCGAGACGATGCACCAGGTCGACGAATGCGTGGCGGTCGCCGACC
TCGAGCGGCTGACCGCGATCTACGGGCGCGTGCTCGACGCCTATTTCCCGGGATCGTAG

Upstream 100 bases:

>100_bases
AGGTCGTGGTCACCAAGTAGGGCCCGAGTCGGGAGTTCCGGGGCGGCCCGCTTCGGGTTAAGCGGGCCGCGCGGCACCTC
GCGCCGCCGAGGACGGGTTC

Downstream 100 bases:

>100_bases
TCGACGCCCACGAAGGTCAGCCCCGCGGCCGGGGCCAGCGGCCCGCAGCGGCTTTTGTCGCCCGAGGCGAGGATCTCGGC
CACGTCGTCCGCGGCGAGGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPADHHAPDAALRLAQDLIRCPSVTPEDRGALDVVADALRPAGFAIERPVFAEPGYPDTPNLYARIGQNGPCLVFAGHTD
VVPEGEGAWRHDPFAGAVADGMLYGRGAADMKGGVACMLAATLAFLDRRGADFGGSIAFLITGDEEGPAVNGTVKLLDWA
RRRGERFDHCVLGEPTNPGRLGEMIKIGRRGSLTGKLTVLGRQGHVAYPHKAENPIPGLLRLASALIAEPLDRGTAHFDA
SNLEFTTVDVGNPATNVIPSTARATFNVRFNDDWTAESLGAEIRRRLEQAAGNAVRFTLDLQPSNAPAFLTRPDAFVTLV
AEAIRAETGLTPTLSTTGGTSDARFIKDACPVIEFGLVGETMHQVDECVAVADLERLTAIYGRVLDAYFPGS

Sequences:

>Translated_392_residues
MPADHHAPDAALRLAQDLIRCPSVTPEDRGALDVVADALRPAGFAIERPVFAEPGYPDTPNLYARIGQNGPCLVFAGHTD
VVPEGEGAWRHDPFAGAVADGMLYGRGAADMKGGVACMLAATLAFLDRRGADFGGSIAFLITGDEEGPAVNGTVKLLDWA
RRRGERFDHCVLGEPTNPGRLGEMIKIGRRGSLTGKLTVLGRQGHVAYPHKAENPIPGLLRLASALIAEPLDRGTAHFDA
SNLEFTTVDVGNPATNVIPSTARATFNVRFNDDWTAESLGAEIRRRLEQAAGNAVRFTLDLQPSNAPAFLTRPDAFVTLV
AEAIRAETGLTPTLSTTGGTSDARFIKDACPVIEFGLVGETMHQVDECVAVADLERLTAIYGRVLDAYFPGS
>Mature_391_residues
PADHHAPDAALRLAQDLIRCPSVTPEDRGALDVVADALRPAGFAIERPVFAEPGYPDTPNLYARIGQNGPCLVFAGHTDV
VPEGEGAWRHDPFAGAVADGMLYGRGAADMKGGVACMLAATLAFLDRRGADFGGSIAFLITGDEEGPAVNGTVKLLDWAR
RRGERFDHCVLGEPTNPGRLGEMIKIGRRGSLTGKLTVLGRQGHVAYPHKAENPIPGLLRLASALIAEPLDRGTAHFDAS
NLEFTTVDVGNPATNVIPSTARATFNVRFNDDWTAESLGAEIRRRLEQAAGNAVRFTLDLQPSNAPAFLTRPDAFVTLVA
EAIRAETGLTPTLSTTGGTSDARFIKDACPVIEFGLVGETMHQVDECVAVADLERLTAIYGRVLDAYFPGS

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=377, Percent_Identity=42.1750663129974, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1790395, Length=379, Percent_Identity=27.9683377308707, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_METRJ (B1M6P0)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001755814.1
- ProteinModelPortal:   B1M6P0
- SMR:   B1M6P0
- MEROPS:   M20.010
- GeneID:   6139196
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3149
- HOGENOM:   HBG728841
- OMA:   ARNPVHQ
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41706; Mature: 41574

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 80-80 ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPADHHAPDAALRLAQDLIRCPSVTPEDRGALDVVADALRPAGFAIERPVFAEPGYPDTP
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCC
NLYARIGQNGPCLVFAGHTDVVPEGEGAWRHDPFAGAVADGMLYGRGAADMKGGVACMLA
HHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCHHHHHH
ATLAFLDRRGADFGGSIAFLITGDEEGPAVNGTVKLLDWARRRGERFDHCVLGEPTNPGR
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCH
LGEMIKIGRRGSLTGKLTVLGRQGHVAYPHKAENPIPGLLRLASALIAEPLDRGTAHFDA
HHHHHHCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECC
SNLEFTTVDVGNPATNVIPSTARATFNVRFNDDWTAESLGAEIRRRLEQAAGNAVRFTLD
CCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
LQPSNAPAFLTRPDAFVTLVAEAIRAETGLTPTLSTTGGTSDARFIKDACPVIEFGLVGE
ECCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHCCCHHHHHHHHH
TMHQVDECVAVADLERLTAIYGRVLDAYFPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PADHHAPDAALRLAQDLIRCPSVTPEDRGALDVVADALRPAGFAIERPVFAEPGYPDTP
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCCCCC
NLYARIGQNGPCLVFAGHTDVVPEGEGAWRHDPFAGAVADGMLYGRGAADMKGGVACMLA
HHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEECCCCCCCCCCHHHHHH
ATLAFLDRRGADFGGSIAFLITGDEEGPAVNGTVKLLDWARRRGERFDHCVLGEPTNPGR
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCH
LGEMIKIGRRGSLTGKLTVLGRQGHVAYPHKAENPIPGLLRLASALIAEPLDRGTAHFDA
HHHHHHCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEECC
SNLEFTTVDVGNPATNVIPSTARATFNVRFNDDWTAESLGAEIRRRLEQAAGNAVRFTLD
CCCEEEEEECCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEEE
LQPSNAPAFLTRPDAFVTLVAEAIRAETGLTPTLSTTGGTSDARFIKDACPVIEFGLVGE
ECCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHCCCHHHHHHHHH
TMHQVDECVAVADLERLTAIYGRVLDAYFPGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA