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The map label for this gene is pckA

Identifier: 170749549

GI number: 170749549

Start: 3324229

End: 3325848

Strand: Reverse

Name: pckA

Synonym: Mrad2831_3144

Alternate gene names: 170749549

Gene position: 3325848-3324229 (Counterclockwise)

Preceding gene: 170749554

Following gene: 170749545

Centisome position: 54.72

GC content: 68.58

Gene sequence:

>1620_bases
TTGACCAACATCGGTGATCACAACGCGGCCCACGGCGCCGAGGCGGCCGGCTTCCGCGACCTCAAGGCCGTCCACTGGAA
CTTCGAGGCCCCGCGCCTCTACGAGGAGGCCCTGTCCCGCAAGGAGGCGCAGCTCGCCCGCGGGGGCGCGATCGTCGCCA
CCACCGGCAGCCATACCGGCCGCTCGCCCAAGGACAAGTTCGTGGTCCGCGACGCCGGCACCGAGAACGAGGTCTGGTGG
GACAACAACGGCGCCATCACGCCCGAGCAGTTCGAGACCCTGCGCCAGGACTTCCTGAAGCACGCCGAGGGCAGGGAGCT
GTTCGCCCAGGACCTCTACGGCGGTGCCGATCCGGCCTACCGGGTGAAGGCGCGGGTGTTCACCGAGTTCGCGTGGCACT
CGCTGTTCATCCGCAACCTGCTCATCCGGCCGGACCGCTCCGAGATCGCGTCCTACGTGCCGGACATGACGATCATCGAC
CTGCCGAGCTTCCAGGCGGATCCCGCCCGGCACGGCTGCCGGTCGAAGACCGTGATCGCCATCGACTTCTCGAAGAAGCT
CGTGCTGATCGGCGGCTCCGCCTACGCGGGCGAGATGAAGAAGTCGGTCTTCACCTACCTGAACTACATCCTGCCCGGTC
AGGGCGTGATGCCGATGCACTGCTCGGCCAACGCGGCGCTCGACGCCGAGGGCGGCTCGGCGATCTTCTTCGGCCTGTCC
GGGACCGGCAAGACGACGCTGTCGAACGATTCCTCCCGTCAGCTGCTCGGCGACGACGAGCACGGCTGGTCGAATTCCGG
GATCTTCAACTTCGAGGGCGGCTGCTACGCCAAGACGATCCGGCTCTCGCGCAACGCCGAGCCGGAGATCTACGCCACCA
CCGAGCGCTTCGGCACGGTGATGGAGAACGTGATCATCGACCCGGTCACCCGCGTGCCGGACTTCGACGACGCGTCGCTC
ACCGAGAATACCCGCTGCGCCTACCCGCTGGACTTCATCGCCAACGCGAGCGCGACGGGCCGGGCCGGGCATCCGAAGAA
CATCGTGATGCTGACCTGCGACGCCTTCGGGGTGATGCCGCCGATCGCCAAGCTCACCGGCGCCGAGGCGATGTACCACT
TCCTCTCGGGCTACACCGCCAAGGTGGCCGGCACCGAGCGCGGCCTGACCGGGCCGGAGGCGACCTTCTCGACCTGCTTC
GGTGCGCCGTTCATGCCGCGGCACCCGAGCACCTACGGCAACCTGCTGCGCGACCTCATCGCCAAGCACAGCGTCGATTG
CTGGCTGGTCAACACCGGCTGGACCGGCGGCGGCGTCGGCACCGGCCGGCGGATGCCGATCCGCGTGACGCGCCGCCTGC
TGAGCGCCGCCCTCGACGGCTCGCTGGCGCAGGCCGAGTTCCGGCGTGACCCGTATTTCGGCTTCGCGGTGCCGGTCTCG
GTTCCGGGCGTCGAGCCGCACATCCTGACCCCGGTGAAGACCTGGGCCAACAAGGGTGCCTTCGTGGAGACGGCCGCGCG
GCTGGTGAAGATGTTCGACGACAACTTCAAGCGGTTCGAGGACCATGTCGACGCCGACGTGCGCTCTGCCGGGCCGACCG
CGCAGGCGATCGCGGCCTGA

Upstream 100 bases:

>100_bases
TCCTGTACGACCGCACGGACGTCTCCCGGGCAAGCCCGGACGGCGGTGCCGTCCGGTTCAGTCGCGTCGACGGTCGAACA
GGGGCCCGGGAGATACGATC

Downstream 100 bases:

>100_bases
AGATCGGACGGGCGGCTCGCAGGGCCGCCCGTTCCTCCCCGCCCTCGCGAGCGCGACGAAGCAGTCCAGGGCAGCGCGTC
CTCGACGAGCGCGGCGCTGC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MTNIGDHNAAHGAEAAGFRDLKAVHWNFEAPRLYEEALSRKEAQLARGGAIVATTGSHTGRSPKDKFVVRDAGTENEVWW
DNNGAITPEQFETLRQDFLKHAEGRELFAQDLYGGADPAYRVKARVFTEFAWHSLFIRNLLIRPDRSEIASYVPDMTIID
LPSFQADPARHGCRSKTVIAIDFSKKLVLIGGSAYAGEMKKSVFTYLNYILPGQGVMPMHCSANAALDAEGGSAIFFGLS
GTGKTTLSNDSSRQLLGDDEHGWSNSGIFNFEGGCYAKTIRLSRNAEPEIYATTERFGTVMENVIIDPVTRVPDFDDASL
TENTRCAYPLDFIANASATGRAGHPKNIVMLTCDAFGVMPPIAKLTGAEAMYHFLSGYTAKVAGTERGLTGPEATFSTCF
GAPFMPRHPSTYGNLLRDLIAKHSVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQAEFRRDPYFGFAVPVS
VPGVEPHILTPVKTWANKGAFVETAARLVKMFDDNFKRFEDHVDADVRSAGPTAQAIAA

Sequences:

>Translated_539_residues
MTNIGDHNAAHGAEAAGFRDLKAVHWNFEAPRLYEEALSRKEAQLARGGAIVATTGSHTGRSPKDKFVVRDAGTENEVWW
DNNGAITPEQFETLRQDFLKHAEGRELFAQDLYGGADPAYRVKARVFTEFAWHSLFIRNLLIRPDRSEIASYVPDMTIID
LPSFQADPARHGCRSKTVIAIDFSKKLVLIGGSAYAGEMKKSVFTYLNYILPGQGVMPMHCSANAALDAEGGSAIFFGLS
GTGKTTLSNDSSRQLLGDDEHGWSNSGIFNFEGGCYAKTIRLSRNAEPEIYATTERFGTVMENVIIDPVTRVPDFDDASL
TENTRCAYPLDFIANASATGRAGHPKNIVMLTCDAFGVMPPIAKLTGAEAMYHFLSGYTAKVAGTERGLTGPEATFSTCF
GAPFMPRHPSTYGNLLRDLIAKHSVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQAEFRRDPYFGFAVPVS
VPGVEPHILTPVKTWANKGAFVETAARLVKMFDDNFKRFEDHVDADVRSAGPTAQAIAA
>Mature_538_residues
TNIGDHNAAHGAEAAGFRDLKAVHWNFEAPRLYEEALSRKEAQLARGGAIVATTGSHTGRSPKDKFVVRDAGTENEVWWD
NNGAITPEQFETLRQDFLKHAEGRELFAQDLYGGADPAYRVKARVFTEFAWHSLFIRNLLIRPDRSEIASYVPDMTIIDL
PSFQADPARHGCRSKTVIAIDFSKKLVLIGGSAYAGEMKKSVFTYLNYILPGQGVMPMHCSANAALDAEGGSAIFFGLSG
TGKTTLSNDSSRQLLGDDEHGWSNSGIFNFEGGCYAKTIRLSRNAEPEIYATTERFGTVMENVIIDPVTRVPDFDDASLT
ENTRCAYPLDFIANASATGRAGHPKNIVMLTCDAFGVMPPIAKLTGAEAMYHFLSGYTAKVAGTERGLTGPEATFSTCFG
APFMPRHPSTYGNLLRDLIAKHSVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQAEFRRDPYFGFAVPVSV
PGVEPHILTPVKTWANKGAFVETAARLVKMFDDNFKRFEDHVDADVRSAGPTAQAIAA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=534, Percent_Identity=43.4456928838951, Blast_Score=448, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6322950, Length=528, Percent_Identity=43.5606060606061, Blast_Score=454, Evalue=1e-128,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_METRJ (B1M6N5)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001755809.1
- ProteinModelPortal:   B1M6N5
- SMR:   B1M6N5
- GeneID:   6139191
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_3144
- HOGENOM:   HBG293387
- OMA:   FAQDLYG
- ProtClustDB:   PRK09344
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 58720; Mature: 58589

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNIGDHNAAHGAEAAGFRDLKAVHWNFEAPRLYEEALSRKEAQLARGGAIVATTGSHTG
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RSPKDKFVVRDAGTENEVWWDNNGAITPEQFETLRQDFLKHAEGRELFAQDLYGGADPAY
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
RVKARVFTEFAWHSLFIRNLLIRPDRSEIASYVPDMTIIDLPSFQADPARHGCRSKTVIA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCEEEE
IDFSKKLVLIGGSAYAGEMKKSVFTYLNYILPGQGVMPMHCSANAALDAEGGSAIFFGLS
EECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCEEEEEEC
GTGKTTLSNDSSRQLLGDDEHGWSNSGIFNFEGGCYAKTIRLSRNAEPEIYATTERFGTV
CCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHH
MENVIIDPVTRVPDFDDASLTENTRCAYPLDFIANASATGRAGHPKNIVMLTCDAFGVMP
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCC
PIAKLTGAEAMYHFLSGYTAKVAGTERGLTGPEATFSTCFGAPFMPRHPSTYGNLLRDLI
CHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
AKHSVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQAEFRRDPYFGFAVPVS
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEE
VPGVEPHILTPVKTWANKGAFVETAARLVKMFDDNFKRFEDHVDADVRSAGPTAQAIAA
CCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCC
>Mature Secondary Structure 
TNIGDHNAAHGAEAAGFRDLKAVHWNFEAPRLYEEALSRKEAQLARGGAIVATTGSHTG
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
RSPKDKFVVRDAGTENEVWWDNNGAITPEQFETLRQDFLKHAEGRELFAQDLYGGADPAY
CCCCCCEEEECCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHH
RVKARVFTEFAWHSLFIRNLLIRPDRSEIASYVPDMTIIDLPSFQADPARHGCRSKTVIA
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCCCCHHHCCCCCCEEEE
IDFSKKLVLIGGSAYAGEMKKSVFTYLNYILPGQGVMPMHCSANAALDAEGGSAIFFGLS
EECCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEECCCCCEEEEEEC
GTGKTTLSNDSSRQLLGDDEHGWSNSGIFNFEGGCYAKTIRLSRNAEPEIYATTERFGTV
CCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHH
MENVIIDPVTRVPDFDDASLTENTRCAYPLDFIANASATGRAGHPKNIVMLTCDAFGVMP
HHHHHHCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECCCCCCC
PIAKLTGAEAMYHFLSGYTAKVAGTERGLTGPEATFSTCFGAPFMPRHPSTYGNLLRDLI
CHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHH
AKHSVDCWLVNTGWTGGGVGTGRRMPIRVTRRLLSAALDGSLAQAEFRRDPYFGFAVPVS
HHCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEE
VPGVEPHILTPVKTWANKGAFVETAARLVKMFDDNFKRFEDHVDADVRSAGPTAQAIAA
CCCCCCCEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA