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The map label for this gene is bepF [H]

Identifier: 170749450

GI number: 170749450

Start: 3210503

End: 3211747

Strand: Reverse

Name: bepF [H]

Synonym: Mrad2831_3045

Alternate gene names: 170749450

Gene position: 3211747-3210503 (Counterclockwise)

Preceding gene: 170749452

Following gene: 170749449

Centisome position: 52.84

GC content: 73.33

Gene sequence:

>1245_bases
GTGGAACCTCAGCGTCATCCCTCTCGCGGTCCTCTTCCCGTCCGGCGCTTCGCCGCCGGCGGTCTCGCCATCGCACTTCT
GGCGGGCGCTGCCTACGCTCTCGTACCGGGGACGCTGATCCGAGGAACCGCGGCCATCGCCGCGACGCCACCGGCCGAGG
TGGCGATGCCGGCCGCGGTCGCGACCGTCGAGCCGCGCGACGTCATCCTCTGGGACGAGTTCTCGGGGCGCCTGGAAGCG
GTCGAACGCATCGACGTGCGCGCCCGGGTCGGCGGTGCGATCCTGGCCACGCATTTCGCCGAAGGCGAGCTGGTCAAGGC
CGGGGACCTGCTGGTGACCATCGACCCCGCGCCCTACGCGGCCGAGGTCCAGAGGCTCGAGGCCCAGGTCGCGGGCGCCG
AGGCGCGCGTCGGCCTGACGGCGAGCGACCTCGAGCGTGGGCAGAAGCTGTCGGATCAGCACATCGTTACGGCACGCGAC
CTCGACGTCCGGGCCAACGCGTTCAAGGAGGCTCGGGCGAGCCTCGACGCGGCGAAGGCGGCGCTGGAGGCCGCGCGCCT
CAACCTCGGCTACACGCAGGTGCGGGCTGCGGTGAGCGGCCGCGTCGGCCGCCGCGAGATCACGCCGGGCAACCTCGTGG
CGACGGGCGCGGCGGCCGCCGTGCTGACCACGCTGGTCTCGGTCGATCCCGTCTACGCCAGCTTCGACGCCGACGAGGGC
GTGGTCCTGAAGGCGCTGGCCTCGGTCGCCGAGCCGGGCGGCCGGCGCGGCAAGCTCGGCCTCATCCCCGTCGAGATGGC
GACCGCCGACGGCGCGCGCGCCAGCGGGCATCTCCAGTTCATCGACAACAAGGTCGATGCGCGCAGCGGCACGGTCCGCG
TGCGCGCGACCTTCTCCAACGGTGACGGCCGCCTGATCCCGGGCCAGTTCGCGCGGATGCGCCTCGGTCAGGCCGCTCCG
GAACGCCTCGTCCTCGTCGACGAACGGGCCGTCGGCACCGATCAGGACAAGAAGTTCGTGCTCGCGGTAGGGGCGGACAA
CCGGGCCGAGTTCCGCGCCGTGACCCTCGGTCGAGCCGTCGACGGACTCCGCGTGGTCACCTCCGGCCTGTCGGGCGGCG
AGCGCATCGTGGTCAACGGCCTGCAGCGCGTCCGTCCCGGGACACTCCTGATCCCCGAGCCCGTGGCGATGGGAGCGCGG
CCGCAGGACAGCGGGTCCGGACCGCGCAAGCTCGCCCAGCGCTGA

Upstream 100 bases:

>100_bases
CTCTGGACTCCAACTTTTATATCGAGTGGTAGATAAGTCACTTGACGCCCCGCGTCCATGCCGACATGTGTAGCGACCTA
TACAGATGTCGGAGTCGCTC

Downstream 100 bases:

>100_bases
CGGAAGCCGCTCGGGCGCGCCTCCCTTCCCCCGAGTCAGATCCCCGACGCGACGATGACGGCGGCCGCCTACGGCCGCCG
GGACGCCCCTGCCTCGCGCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MEPQRHPSRGPLPVRRFAAGGLAIALLAGAAYALVPGTLIRGTAAIAATPPAEVAMPAAVATVEPRDVILWDEFSGRLEA
VERIDVRARVGGAILATHFAEGELVKAGDLLVTIDPAPYAAEVQRLEAQVAGAEARVGLTASDLERGQKLSDQHIVTARD
LDVRANAFKEARASLDAAKAALEAARLNLGYTQVRAAVSGRVGRREITPGNLVATGAAAAVLTTLVSVDPVYASFDADEG
VVLKALASVAEPGGRRGKLGLIPVEMATADGARASGHLQFIDNKVDARSGTVRVRATFSNGDGRLIPGQFARMRLGQAAP
ERLVLVDERAVGTDQDKKFVLAVGADNRAEFRAVTLGRAVDGLRVVTSGLSGGERIVVNGLQRVRPGTLLIPEPVAMGAR
PQDSGSGPRKLAQR

Sequences:

>Translated_414_residues
MEPQRHPSRGPLPVRRFAAGGLAIALLAGAAYALVPGTLIRGTAAIAATPPAEVAMPAAVATVEPRDVILWDEFSGRLEA
VERIDVRARVGGAILATHFAEGELVKAGDLLVTIDPAPYAAEVQRLEAQVAGAEARVGLTASDLERGQKLSDQHIVTARD
LDVRANAFKEARASLDAAKAALEAARLNLGYTQVRAAVSGRVGRREITPGNLVATGAAAAVLTTLVSVDPVYASFDADEG
VVLKALASVAEPGGRRGKLGLIPVEMATADGARASGHLQFIDNKVDARSGTVRVRATFSNGDGRLIPGQFARMRLGQAAP
ERLVLVDERAVGTDQDKKFVLAVGADNRAEFRAVTLGRAVDGLRVVTSGLSGGERIVVNGLQRVRPGTLLIPEPVAMGAR
PQDSGSGPRKLAQR
>Mature_414_residues
MEPQRHPSRGPLPVRRFAAGGLAIALLAGAAYALVPGTLIRGTAAIAATPPAEVAMPAAVATVEPRDVILWDEFSGRLEA
VERIDVRARVGGAILATHFAEGELVKAGDLLVTIDPAPYAAEVQRLEAQVAGAEARVGLTASDLERGQKLSDQHIVTARD
LDVRANAFKEARASLDAAKAALEAARLNLGYTQVRAAVSGRVGRREITPGNLVATGAAAAVLTTLVSVDPVYASFDADEG
VVLKALASVAEPGGRRGKLGLIPVEMATADGARASGHLQFIDNKVDARSGTVRVRATFSNGDGRLIPGQFARMRLGQAAP
ERLVLVDERAVGTDQDKKFVLAVGADNRAEFRAVTLGRAVDGLRVVTSGLSGGERIVVNGLQRVRPGTLLIPEPVAMGAR
PQDSGSGPRKLAQR

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=363, Percent_Identity=35.2617079889807, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789929, Length=331, Percent_Identity=33.5347432024169, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI87082036, Length=314, Percent_Identity=29.6178343949045, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=30.4582210242588, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87081951, Length=266, Percent_Identity=27.0676691729323, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081796, Length=183, Percent_Identity=28.9617486338798, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43165; Mature: 43165

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPQRHPSRGPLPVRRFAAGGLAIALLAGAAYALVPGTLIRGTAAIAATPPAEVAMPAAV
CCCCCCCCCCCCCHHHHHCCCHHHHHHHCCHHHHCCCHHHCCCEEEECCCCHHHCCCEEE
ATVEPRDVILWDEFSGRLEAVERIDVRARVGGAILATHFAEGELVKAGDLLVTIDPAPYA
EEECCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCEEEEECCCCCH
AEVQRLEAQVAGAEARVGLTASDLERGQKLSDQHIVTARDLDVRANAFKEARASLDAAKA
HHHHHHHHHHCCCCEEECCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
ALEAARLNLGYTQVRAAVSGRVGRREITPGNLVATGAAAAVLTTLVSVDPVYASFDADEG
HHHHHHHCCCHHHHHHHHHCCCCCEECCCCCEEECHHHHHHHHHHHHCCCEEEECCCCCC
VVLKALASVAEPGGRRGKLGLIPVEMATADGARASGHLQFIDNKVDARSGTVRVRATFSN
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEC
GDGRLIPGQFARMRLGQAAPERLVLVDERAVGTDQDKKFVLAVGADNRAEFRAVTLGRAV
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHH
DGLRVVTSGLSGGERIVVNGLQRVRPGTLLIPEPVAMGARPQDSGSGPRKLAQR
CCHHHEECCCCCCCEEEEECHHHCCCCEEECCCHHHCCCCCCCCCCCHHHHHCC
>Mature Secondary Structure
MEPQRHPSRGPLPVRRFAAGGLAIALLAGAAYALVPGTLIRGTAAIAATPPAEVAMPAAV
CCCCCCCCCCCCCHHHHHCCCHHHHHHHCCHHHHCCCHHHCCCEEEECCCCHHHCCCEEE
ATVEPRDVILWDEFSGRLEAVERIDVRARVGGAILATHFAEGELVKAGDLLVTIDPAPYA
EEECCCEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCEEECCCEEEEECCCCCH
AEVQRLEAQVAGAEARVGLTASDLERGQKLSDQHIVTARDLDVRANAFKEARASLDAAKA
HHHHHHHHHHCCCCEEECCCHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
ALEAARLNLGYTQVRAAVSGRVGRREITPGNLVATGAAAAVLTTLVSVDPVYASFDADEG
HHHHHHHCCCHHHHHHHHHCCCCCEECCCCCEEECHHHHHHHHHHHHCCCEEEECCCCCC
VVLKALASVAEPGGRRGKLGLIPVEMATADGARASGHLQFIDNKVDARSGTVRVRATFSN
HHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEC
GDGRLIPGQFARMRLGQAAPERLVLVDERAVGTDQDKKFVLAVGADNRAEFRAVTLGRAV
CCCCCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCCEEEEEEHHHHH
DGLRVVTSGLSGGERIVVNGLQRVRPGTLLIPEPVAMGARPQDSGSGPRKLAQR
CCHHHEECCCCCCCEEEEECHHHCCCCEEECCCHHHCCCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]