Click here to switch to the map view.

The map label for this gene is rph

Identifier: 170749350

GI number: 170749350

Start: 3101786

End: 3102499

Strand: Reverse

Name: rph

Synonym: Mrad2831_2943

Alternate gene names: 170749350

Gene position: 3102499-3101786 (Counterclockwise)

Preceding gene: 170749352

Following gene: 170749349

Centisome position: 51.05

GC content: 71.43

Gene sequence:

>714_bases
ATGCGGCCTTCCAAGCGTGCCGCCGACGAGTTGCGGCCCGTGAGCCTGGAGCGCGCGGTCTCGCGCTACGCCGAGGGCTC
GTGCCTCGTCAGTTTCGGCAACACCCGGGTGCTCTGCACCGCGTCCCTGGAAGAGCGCGCGCCGCCGTGGCTGCGCGGTT
CCGGCAAGGGCTGGGTCACGGCCGAGTACGCCATGCTCCCGCGTGCGACGCACGAACGCACCCGCCGCGAGGTCGGTTCC
GGGAAACCGTCGGGCCGGACCCAGGAGATCCAGCGGCTGATCGGCCGGTCGCTGCGCGCCGTCACCAACCTGCCGGCCAT
GGGCGAGCGCCAGATCACGATCGACTGCGACGTGATCCAGGCCGACGGCGGCACTCGGACGGCCGCGATCACGGGCGCCT
GGGTGGCGCTGCACGATTGCTTCGCCTGGATGCGGACGCGCTCGATCATCTCCGTCGATCCGCTGCGCGACCACGTCGCC
GCCGTCTCGTGCGGCCTGTACAAGGGCACGCCGGTTCTCGACCTCGACTACGCCGAGGATTCGGCGGCCGAGACCGACGC
CAACTTTGTGCTCACGGGCCGGGGCGGCATCGTCGAGGTGCAGGGCACCGCCGAGATGGAGCCCTTCACGCAGGAACAGC
TCCTCGAACTCCTCGGCCTCGCCCGCGCCGGCACGGAGCGGCTGGTCGCCCTGCAGAAGGAGGCCATCGCGTGA

Upstream 100 bases:

>100_bases
AACCGTCCCGCGCGGATCAATCCGCACGGCGATGCTTGCCCGTGGTTGCGCGGCGGCCTAAGAGCGCGGCCCTCGACACT
CTCGACAGAAGGGGCCCGCG

Downstream 100 bases:

>100_bases
GCCGCCGTCTGACCGGGAAGGTGGTGATCGCCACGCACAATGCCGGCAAACTCACCGAGATGCGCGAGCTGCTCGCGCCG
TTCGGAATCGAGGCGGTGTC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MRPSKRAADELRPVSLERAVSRYAEGSCLVSFGNTRVLCTASLEERAPPWLRGSGKGWVTAEYAMLPRATHERTRREVGS
GKPSGRTQEIQRLIGRSLRAVTNLPAMGERQITIDCDVIQADGGTRTAAITGAWVALHDCFAWMRTRSIISVDPLRDHVA
AVSCGLYKGTPVLDLDYAEDSAAETDANFVLTGRGGIVEVQGTAEMEPFTQEQLLELLGLARAGTERLVALQKEAIA

Sequences:

>Translated_237_residues
MRPSKRAADELRPVSLERAVSRYAEGSCLVSFGNTRVLCTASLEERAPPWLRGSGKGWVTAEYAMLPRATHERTRREVGS
GKPSGRTQEIQRLIGRSLRAVTNLPAMGERQITIDCDVIQADGGTRTAAITGAWVALHDCFAWMRTRSIISVDPLRDHVA
AVSCGLYKGTPVLDLDYAEDSAAETDANFVLTGRGGIVEVQGTAEMEPFTQEQLLELLGLARAGTERLVALQKEAIA
>Mature_237_residues
MRPSKRAADELRPVSLERAVSRYAEGSCLVSFGNTRVLCTASLEERAPPWLRGSGKGWVTAEYAMLPRATHERTRREVGS
GKPSGRTQEIQRLIGRSLRAVTNLPAMGERQITIDCDVIQADGGTRTAAITGAWVALHDCFAWMRTRSIISVDPLRDHVA
AVSCGLYKGTPVLDLDYAEDSAAETDANFVLTGRGGIVEVQGTAEMEPFTQEQLLELLGLARAGTERLVALQKEAIA

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=58.2938388625592, Blast_Score=255, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI71981632, Length=181, Percent_Identity=27.6243093922652, Blast_Score=74, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_METRJ (B1M4N1)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001755610.1
- ProteinModelPortal:   B1M4N1
- SMR:   B1M4N1
- GeneID:   6138987
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_2943
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25753; Mature: 25753

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSKRAADELRPVSLERAVSRYAEGSCLVSFGNTRVLCTASLEERAPPWLRGSGKGWVT
CCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEECCEEEEEEECHHHCCCCCEECCCCCCEE
AEYAMLPRATHERTRREVGSGKPSGRTQEIQRLIGRSLRAVTNLPAMGERQITIDCDVIQ
EHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
ADGGTRTAAITGAWVALHDCFAWMRTRSIISVDPLRDHVAAVSCGLYKGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCEEEEECCCC
SAAETDANFVLTGRGGIVEVQGTAEMEPFTQEQLLELLGLARAGTERLVALQKEAIA
CCCCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSKRAADELRPVSLERAVSRYAEGSCLVSFGNTRVLCTASLEERAPPWLRGSGKGWVT
CCCCHHHHHHCCCCHHHHHHHHHCCCCEEEEECCEEEEEEECHHHCCCCCEECCCCCCEE
AEYAMLPRATHERTRREVGSGKPSGRTQEIQRLIGRSLRAVTNLPAMGERQITIDCDVIQ
EHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEEE
ADGGTRTAAITGAWVALHDCFAWMRTRSIISVDPLRDHVAAVSCGLYKGTPVLDLDYAED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHCCCCCCCCEEEEECCCC
SAAETDANFVLTGRGGIVEVQGTAEMEPFTQEQLLELLGLARAGTERLVALQKEAIA
CCCCCCCCEEEECCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA