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The map label for this gene is nasA [H]

Identifier: 170749323

GI number: 170749323

Start: 3073862

End: 3075112

Strand: Reverse

Name: nasA [H]

Synonym: Mrad2831_2916

Alternate gene names: 170749323

Gene position: 3075112-3073862 (Counterclockwise)

Preceding gene: 170749325

Following gene: 170749322

Centisome position: 50.6

GC content: 68.19

Gene sequence:

>1251_bases
ATGGCCAGCTTCAAAACGGTGATGAAGTCGGGTCACCCCCCGACGCTGTTCGCGGCATTCCTCTACTTCGATTTCTGTTT
CGCCATCTGGGTCCTGAACGGCGCCATGGGGCCGTTCATCACCGAGACCTTCAAGCTCTCGCCGGCCCAGACCGGCTTCA
TGATCTCGCTGCCGATCCTCGCCGGAGCGCTGATGCGCTTCCCGCTCGGCGTTCTGGCCCAGTATATCGGCCGGAAGAAC
GCCGCGATCACCGAGATGAGCGTGATCATGCTGGCGATGGCCTACGGCTATCTCTTCGTCTCCTCCTACAACGACGTCCT
CGCCATGGGCGTCCTGCTCGGCATCGCCGGCGCGTCCTTCGGCGTCGCGCTGTCCCTCGGCTCGGGCTGGTTCCCGCCGG
AGCACAAGGGGCTGGCGATGGGCATCGCGGGCGCCGGCAATTCCGGCACCGTGCTGGCCGTGCTGTTCGCCCCGCCGCTG
GCCCAGGCCTACGGCTGGCAGACCACCTACGCCTTCGCGGGCGTCGTCATGCTGCTGCCCCTCGCGATCATGATCGTGCT
CGCCAAGGAGCCGCCGGACTGCGAGCACCAGACCTTCAAGGAGCACGTCTCCTGTCTGTTCACCAAGGACGGCTGGGCGT
TCTCGCTGATCTACGTCATCACCTTCGGCGGCTTCATCGGCCTCTCGAACTTCCTGCCGACCTTCTTCTACGAGCAGTTC
GCGGTGACGAAGGTCGAGGCCGGGCGCCTGACCATGCTGGCGGCCTTCATGGGCTCGGGCATCCGGATCGTCGGGGGCTA
CTTCGCCGACCGGATGGGCGGGATCGCGGTCCTGTCGGTGGTGCTGCTCGCGGCGATCGCCACGCTGCTGTCGCTGACCG
CGACCCCCTCCCTGGCGCTCACGACGCTCCTGTTCATGCTGTGCTTCGCGGCCCTCGGGGCCGGCAACGGCGCGCTGTTC
CAGCTCGTTCCGCTGCGCTGGCCGACCAACACGGCCGTGGCCGGCTCGATGATCGGCGAGATCGGGGCGCTGGGCGGCGC
GATCCTGCCGAACGTCATGGGCCTGTCCAAGCAGTATACCGGCTCGTTCTCGCTGGGCTTCGTCGGCTACGCCGCCTTCA
CGGCGGTCGTCCTCGGCTGCCTCGCGCTCTGGCAGCGGAAGTGGGTCGGGAGCTGGGTCGGGCCGCGCGGCAAGGCACTC
GGCACCGCGGCCGAGGCCGAGCAGGGCCAGCTGCGGCCCGCGACCGCCTGA

Upstream 100 bases:

>100_bases
GCGGCGGAGCAACGGCGCTCCAAACAGCGGACGGTGTGAGTCCGGATCCCCGGCATGAGCGACCGCGTGGCGGTTCGCCC
ACTTACGACGAGCACCAGCT

Downstream 100 bases:

>100_bases
CCGCCGCCGCAGCGTCTCGTAGCGGCGAACGACCTGTGACGGGGACCCTGGGGTCCCCGTTTTCAGTTGCAGCGCGAACG
AGGAATTCGCGTGCCGGATG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MASFKTVMKSGHPPTLFAAFLYFDFCFAIWVLNGAMGPFITETFKLSPAQTGFMISLPILAGALMRFPLGVLAQYIGRKN
AAITEMSVIMLAMAYGYLFVSSYNDVLAMGVLLGIAGASFGVALSLGSGWFPPEHKGLAMGIAGAGNSGTVLAVLFAPPL
AQAYGWQTTYAFAGVVMLLPLAIMIVLAKEPPDCEHQTFKEHVSCLFTKDGWAFSLIYVITFGGFIGLSNFLPTFFYEQF
AVTKVEAGRLTMLAAFMGSGIRIVGGYFADRMGGIAVLSVVLLAAIATLLSLTATPSLALTTLLFMLCFAALGAGNGALF
QLVPLRWPTNTAVAGSMIGEIGALGGAILPNVMGLSKQYTGSFSLGFVGYAAFTAVVLGCLALWQRKWVGSWVGPRGKAL
GTAAEAEQGQLRPATA

Sequences:

>Translated_416_residues
MASFKTVMKSGHPPTLFAAFLYFDFCFAIWVLNGAMGPFITETFKLSPAQTGFMISLPILAGALMRFPLGVLAQYIGRKN
AAITEMSVIMLAMAYGYLFVSSYNDVLAMGVLLGIAGASFGVALSLGSGWFPPEHKGLAMGIAGAGNSGTVLAVLFAPPL
AQAYGWQTTYAFAGVVMLLPLAIMIVLAKEPPDCEHQTFKEHVSCLFTKDGWAFSLIYVITFGGFIGLSNFLPTFFYEQF
AVTKVEAGRLTMLAAFMGSGIRIVGGYFADRMGGIAVLSVVLLAAIATLLSLTATPSLALTTLLFMLCFAALGAGNGALF
QLVPLRWPTNTAVAGSMIGEIGALGGAILPNVMGLSKQYTGSFSLGFVGYAAFTAVVLGCLALWQRKWVGSWVGPRGKAL
GTAAEAEQGQLRPATA
>Mature_415_residues
ASFKTVMKSGHPPTLFAAFLYFDFCFAIWVLNGAMGPFITETFKLSPAQTGFMISLPILAGALMRFPLGVLAQYIGRKNA
AITEMSVIMLAMAYGYLFVSSYNDVLAMGVLLGIAGASFGVALSLGSGWFPPEHKGLAMGIAGAGNSGTVLAVLFAPPLA
QAYGWQTTYAFAGVVMLLPLAIMIVLAKEPPDCEHQTFKEHVSCLFTKDGWAFSLIYVITFGGFIGLSNFLPTFFYEQFA
VTKVEAGRLTMLAAFMGSGIRIVGGYFADRMGGIAVLSVVLLAAIATLLSLTATPSLALTTLLFMLCFAALGAGNGALFQ
LVPLRWPTNTAVAGSMIGEIGALGGAILPNVMGLSKQYTGSFSLGFVGYAAFTAVVLGCLALWQRKWVGSWVGPRGKALG
TAAEAEQGQLRPATA

Specific function: May function as a nitrate transporter [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=388, Percent_Identity=26.0309278350515, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787743, Length=387, Percent_Identity=25.8397932816537, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43774; Mature: 43643

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFKTVMKSGHPPTLFAAFLYFDFCFAIWVLNGAMGPFITETFKLSPAQTGFMISLPIL
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEEHHHH
AGALMRFPLGVLAQYIGRKNAAITEMSVIMLAMAYGYLFVSSYNDVLAMGVLLGIAGASF
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
GVALSLGSGWFPPEHKGLAMGIAGAGNSGTVLAVLFAPPLAQAYGWQTTYAFAGVVMLLP
CEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHH
LAIMIVLAKEPPDCEHQTFKEHVSCLFTKDGWAFSLIYVITFGGFIGLSNFLPTFFYEQF
HHHHHHHCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTKVEAGRLTMLAAFMGSGIRIVGGYFADRMGGIAVLSVVLLAAIATLLSLTATPSLAL
HHEEECCCHHHHHHHHHCCCEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHH
TTLLFMLCFAALGAGNGALFQLVPLRWPTNTAVAGSMIGEIGALGGAILPNVMGLSKQYT
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCHHCC
GSFSLGFVGYAAFTAVVLGCLALWQRKWVGSWVGPRGKALGTAAEAEQGQLRPATA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
ASFKTVMKSGHPPTLFAAFLYFDFCFAIWVLNGAMGPFITETFKLSPAQTGFMISLPIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCEEEHHHH
AGALMRFPLGVLAQYIGRKNAAITEMSVIMLAMAYGYLFVSSYNDVLAMGVLLGIAGASF
HHHHHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
GVALSLGSGWFPPEHKGLAMGIAGAGNSGTVLAVLFAPPLAQAYGWQTTYAFAGVVMLLP
CEEEECCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHHHHH
LAIMIVLAKEPPDCEHQTFKEHVSCLFTKDGWAFSLIYVITFGGFIGLSNFLPTFFYEQF
HHHHHHHCCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVTKVEAGRLTMLAAFMGSGIRIVGGYFADRMGGIAVLSVVLLAAIATLLSLTATPSLAL
HHEEECCCHHHHHHHHHCCCEEEEHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHH
TTLLFMLCFAALGAGNGALFQLVPLRWPTNTAVAGSMIGEIGALGGAILPNVMGLSKQYT
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCHHCC
GSFSLGFVGYAAFTAVVLGCLALWQRKWVGSWVGPRGKALGTAAEAEQGQLRPATA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]