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The map label for this gene is pntB [H]

Identifier: 170749205

GI number: 170749205

Start: 2938159

End: 2939556

Strand: Reverse

Name: pntB [H]

Synonym: Mrad2831_2798

Alternate gene names: 170749205

Gene position: 2939556-2938159 (Counterclockwise)

Preceding gene: 170749206

Following gene: 170749204

Centisome position: 48.37

GC content: 67.1

Gene sequence:

>1398_bases
GTGTCAGAGAACGTCTCCTCCCTTCTCTATATCGTCGCCGGCGTCCTGTTCATCATGGCGCTGCGGGGGCTCTCGCACCC
CACAACCTCCCGGCAGGGCAACCTGTACGGCATGGTCGGCATGGGGCTCGCGATCCTGACGACGCTCGTCGGCCACCCGC
CGGCGGGTGCAGGCGCCTGGATCCTCGTGCTGCTGGGCCTCGGCATCGGCGGCGGCGCGGGCGCGGTGATCGCCAAGCGC
GTGCCGATGACCGCGATGCCGCAGCTTGTGGCGGCGTTCCACTCGCTGGTCGGCCTCGCGGCCGTCTTCGTGGCGGCGGG
CGCACTCTACGCCCCGCAGGCCTTCGGCATCATCGAGAACGGCCACTTCCACAAGCAGTCGCTCTTCGAGATGGGTCTCG
GCGTCGCCATCGGCGCCATCACCTTCACCGGCTCGGTGATCGCGTTCGCCAAGCTCGACGGTCGCATGTCCGGCAAGCCG
ATCATGCTGCCCCAGCGCCACCTGATCAACGCGCTGCTGGCCGCCGGCCTCGTGCTGCTGATCGCCCTGTTCATCGGCAC
CGAGTCGAAGGCGATCTTCTGGTTGATCGTCATCGCCTCGCTCGTGCTGGGCGGGCTGATCATCATCCCGATCGGCGGCG
CCGACATGCCGGTGGTCGTGTCGATGCTCAACTCCTACTCGGGCTGGGCGGCGGCGGGCATCGGCTTCACCCTGGGCAAC
CTCGCGCTGATCATCACCGGCTCGCTGGTCGGATCCTCGGGCGCGATCCTGTCGTACATCATGTGCCACGCGATGAACCG
GTCGTTCATCTCGGTCATCCTCGGCGGCTTCGGCGGCGACACGGCCGCGGCCGGCACCGGTCAGGTCGAGACCCGGCCCG
TGAAGCAGGGTTCGGCCGACGACGCGGCCTACATCATGAAGAACGCCGAGCGGGTCATCATCGTCCCGGGCTACGGCATG
GCGGTCGCCCAGGCGCAGCACTCGCTGCGCGAGATGGCCGACATGCTGAAGAAGGAGGGCGTCGACGTGAAGTACGCCAT
CCATCCGGTGGCGGGCCGCATGCCGGGCCACATGAACGTGCTGCTGGCCGAGGCCAACGTGCCCTACGACGAGGTGTTCG
AGTTGGAGGACATCAACGGCGAGTTCCCGCAGGCGGACGTCGCCTTCGTGATCGGCGCCAACGACGTGACGAACCCGGCC
GCCAAGACCGACAAGGCCTCGCCGATCTACGGCATGCCGATCCTGGACGTGGAGAAGGCCAAGACCGTGCTGTTCATCAA
GCGCGGCATGGGCTCCGGCTACGCGGGCGTCGAGAACGAGGTGTTCTTCCGCGACAACACCATGATGCTGTTCGGCGACG
CCAAGAAGGTCGTCGACAACATCCTGAAGTCCCTCTGA

Upstream 100 bases:

>100_bases
TTCGGCTTCATCGGCATCGTGCTCGCCAGCGTGAACATCTTCGGCGGCTTCCTCGTCACGCAACGCATGCTCGGCATGTA
CAAGAAGAAGGGCTGAGCCG

Downstream 100 bases:

>100_bases
GACTCAGGTCTCCGGCAGATCCGACGGGCGGGCAGCGATGCCCGCCCTTTTTAGTTGTGCCGGATCGCGTTCGCCGTCGC
TGTTGCGGCGAGGCGCAAGA

Product: NAD(P)(+) transhydrogenase

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit beta; Proton-translocating transhydrogenase NADP(H)-binding component; Pyridine nucleotide transhydrogenase subunit beta; dIII [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSENVSSLLYIVAGVLFIMALRGLSHPTTSRQGNLYGMVGMGLAILTTLVGHPPAGAGAWILVLLGLGIGGGAGAVIAKR
VPMTAMPQLVAAFHSLVGLAAVFVAAGALYAPQAFGIIENGHFHKQSLFEMGLGVAIGAITFTGSVIAFAKLDGRMSGKP
IMLPQRHLINALLAAGLVLLIALFIGTESKAIFWLIVIASLVLGGLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGN
LALIITGSLVGSSGAILSYIMCHAMNRSFISVILGGFGGDTAAAGTGQVETRPVKQGSADDAAYIMKNAERVIIVPGYGM
AVAQAQHSLREMADMLKKEGVDVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINGEFPQADVAFVIGANDVTNPA
AKTDKASPIYGMPILDVEKAKTVLFIKRGMGSGYAGVENEVFFRDNTMMLFGDAKKVVDNILKSL

Sequences:

>Translated_465_residues
MSENVSSLLYIVAGVLFIMALRGLSHPTTSRQGNLYGMVGMGLAILTTLVGHPPAGAGAWILVLLGLGIGGGAGAVIAKR
VPMTAMPQLVAAFHSLVGLAAVFVAAGALYAPQAFGIIENGHFHKQSLFEMGLGVAIGAITFTGSVIAFAKLDGRMSGKP
IMLPQRHLINALLAAGLVLLIALFIGTESKAIFWLIVIASLVLGGLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGN
LALIITGSLVGSSGAILSYIMCHAMNRSFISVILGGFGGDTAAAGTGQVETRPVKQGSADDAAYIMKNAERVIIVPGYGM
AVAQAQHSLREMADMLKKEGVDVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINGEFPQADVAFVIGANDVTNPA
AKTDKASPIYGMPILDVEKAKTVLFIKRGMGSGYAGVENEVFFRDNTMMLFGDAKKVVDNILKSL
>Mature_464_residues
SENVSSLLYIVAGVLFIMALRGLSHPTTSRQGNLYGMVGMGLAILTTLVGHPPAGAGAWILVLLGLGIGGGAGAVIAKRV
PMTAMPQLVAAFHSLVGLAAVFVAAGALYAPQAFGIIENGHFHKQSLFEMGLGVAIGAITFTGSVIAFAKLDGRMSGKPI
MLPQRHLINALLAAGLVLLIALFIGTESKAIFWLIVIASLVLGGLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGNL
ALIITGSLVGSSGAILSYIMCHAMNRSFISVILGGFGGDTAAAGTGQVETRPVKQGSADDAAYIMKNAERVIIVPGYGMA
VAQAQHSLREMADMLKKEGVDVKYAIHPVAGRMPGHMNVLLAEANVPYDEVFELEDINGEFPQADVAFVIGANDVTNPAA
KTDKASPIYGMPILDVEKAKTVLFIKRGMGSGYAGVENEVFFRDNTMMLFGDAKKVVDNILKSL

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG1282

COG function: function code C; NAD/NADP transhydrogenase beta subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNT beta subunit family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=467, Percent_Identity=45.3961456102784, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI122939153, Length=467, Percent_Identity=45.3961456102784, Blast_Score=372, Evalue=1e-103,
Organism=Escherichia coli, GI1787886, Length=473, Percent_Identity=54.9682875264271, Blast_Score=466, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17550456, Length=473, Percent_Identity=43.3403805496829, Blast_Score=346, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012136
- InterPro:   IPR004003 [H]

Pfam domain/function: PF02233 PNTB [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 48440; Mature: 48309

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENVSSLLYIVAGVLFIMALRGLSHPTTSRQGNLYGMVGMGLAILTTLVGHPPAGAGAW
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHHH
ILVLLGLGIGGGAGAVIAKRVPMTAMPQLVAAFHSLVGLAAVFVAAGALYAPQAFGIIEN
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
GHFHKQSLFEMGLGVAIGAITFTGSVIAFAKLDGRMSGKPIMLPQRHLINALLAAGLVLL
CCCHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
IALFIGTESKAIFWLIVIASLVLGGLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGN
HHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHC
LALIITGSLVGSSGAILSYIMCHAMNRSFISVILGGFGGDTAAAGTGQVETRPVKQGSAD
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DAAYIMKNAERVIIVPGYGMAVAQAQHSLREMADMLKKEGVDVKYAIHPVAGRMPGHMNV
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
LLAEANVPYDEVFELEDINGEFPQADVAFVIGANDVTNPAAKTDKASPIYGMPILDVEKA
EEEECCCCHHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCC
KTVLFIKRGMGSGYAGVENEVFFRDNTMMLFGDAKKVVDNILKSL
CEEEEEEECCCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHCC
>Mature Secondary Structure 
SENVSSLLYIVAGVLFIMALRGLSHPTTSRQGNLYGMVGMGLAILTTLVGHPPAGAGAW
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCHHH
ILVLLGLGIGGGAGAVIAKRVPMTAMPQLVAAFHSLVGLAAVFVAAGALYAPQAFGIIEN
HHHHHHCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECC
GHFHKQSLFEMGLGVAIGAITFTGSVIAFAKLDGRMSGKPIMLPQRHLINALLAAGLVLL
CCCHHHHHHHHCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
IALFIGTESKAIFWLIVIASLVLGGLIIIPIGGADMPVVVSMLNSYSGWAAAGIGFTLGN
HHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHC
LALIITGSLVGSSGAILSYIMCHAMNRSFISVILGGFGGDTAAAGTGQVETRPVKQGSAD
EEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DAAYIMKNAERVIIVPGYGMAVAQAQHSLREMADMLKKEGVDVKYAIHPVAGRMPGHMNV
CHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEE
LLAEANVPYDEVFELEDINGEFPQADVAFVIGANDVTNPAAKTDKASPIYGMPILDVEKA
EEEECCCCHHHHEEEECCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCC
KTVLFIKRGMGSGYAGVENEVFFRDNTMMLFGDAKKVVDNILKSL
CEEEEEEECCCCCCCCCCCCEEEECCEEEEECCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]