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The map label for this gene is narI [H]

Identifier: 170748999

GI number: 170748999

Start: 2743624

End: 2744391

Strand: Reverse

Name: narI [H]

Synonym: Mrad2831_2589

Alternate gene names: 170748999

Gene position: 2744391-2743624 (Counterclockwise)

Preceding gene: 170749000

Following gene: 170748998

Centisome position: 45.15

GC content: 67.19

Gene sequence:

>768_bases
ATGACCTTCGATCTCAACCACGTCCTGTTCGGCTGGTACCCCTATCTCTGCCTCACCGTGTTCCTGGTTGGCTCGCTCGT
GCGCTTCGACCGCGAGCAGTACACGTGGAAGACCGGCTCCAGCCAACTGTTGCGCCGCAAGCAGCTCATCCTCGGCTCGG
TGCTGTTCCACGTCGGCGTCCTGGCCATCTTCGCGGGCCACTTCGTCGGGCTCCTGACGCCCATCGCGGTGTTCGACGCG
CTCGGCGTCGGCCACGGCTTCAAGCAGGGTCTCGCCATCGTGGCGGGTGGCGTCGCCGGCACGGCCTGCTTCGTCGGGCT
TAGCCTGCTGGTGCACCGCCGCCTCTACGACCCGCGCATCCGCGCGACCTCATCCTTCTCGGATACGGCGATCCTGCTGA
TGCTCTATGCCCAGCTCATCCTCGGTCTGTCGACCATCCCGGTCTCGCTCGGGCATATGGACGGGCACGAGATGGTCAAG
TTCATGAACTGGGCCCAGGGCGTGCTCACGCTGCAGCCCGGCGTCGCCGACCTCGTCGCGGACGTGAACCCGGTGTTCAA
GCTGCACCTCGTTCTCGGAATGACGATCTTCCTGGTCTTCCCGTTCACGCGGCTCGTCCACGTCTGGTCGGCGCCGGTCT
GGTACCTCGGCCGTCACGGCTACCAGGTCGTGCGCACGAAGAAGCCGCAGCCCGTGCGGCAGCCCGCGCCGCTGCCTGAC
CAGCACTACGGCGTGCGCCCGGTCCGGGCCCCCCAGCCCGCGGAGTAA

Upstream 100 bases:

>100_bases
CCAGGCCCGCCGCCCGGCCCCCGGCATCGAGGTGCCGGCAGGGCATCCCGCCCGCACAGTCTTCACCCACGTGTCCTCCG
GCGCCTGAGGAGAGCGGGCC

Downstream 100 bases:

>100_bases
GAGCCTTGGGTCCGAGGCAGTCCCGGGCCGCCATCCTACCCGTCCAACACCCTGGGGACCGACCGATGTCCGCCTGCTCC
GTCCAAGCCCTGCCGAACGG

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTFDLNHVLFGWYPYLCLTVFLVGSLVRFDREQYTWKTGSSQLLRRKQLILGSVLFHVGVLAIFAGHFVGLLTPIAVFDA
LGVGHGFKQGLAIVAGGVAGTACFVGLSLLVHRRLYDPRIRATSSFSDTAILLMLYAQLILGLSTIPVSLGHMDGHEMVK
FMNWAQGVLTLQPGVADLVADVNPVFKLHLVLGMTIFLVFPFTRLVHVWSAPVWYLGRHGYQVVRTKKPQPVRQPAPLPD
QHYGVRPVRAPQPAE

Sequences:

>Translated_255_residues
MTFDLNHVLFGWYPYLCLTVFLVGSLVRFDREQYTWKTGSSQLLRRKQLILGSVLFHVGVLAIFAGHFVGLLTPIAVFDA
LGVGHGFKQGLAIVAGGVAGTACFVGLSLLVHRRLYDPRIRATSSFSDTAILLMLYAQLILGLSTIPVSLGHMDGHEMVK
FMNWAQGVLTLQPGVADLVADVNPVFKLHLVLGMTIFLVFPFTRLVHVWSAPVWYLGRHGYQVVRTKKPQPVRQPAPLPD
QHYGVRPVRAPQPAE
>Mature_254_residues
TFDLNHVLFGWYPYLCLTVFLVGSLVRFDREQYTWKTGSSQLLRRKQLILGSVLFHVGVLAIFAGHFVGLLTPIAVFDAL
GVGHGFKQGLAIVAGGVAGTACFVGLSLLVHRRLYDPRIRATSSFSDTAILLMLYAQLILGLSTIPVSLGHMDGHEMVKF
MNWAQGVLTLQPGVADLVADVNPVFKLHLVLGMTIFLVFPFTRLVHVWSAPVWYLGRHGYQVVRTKKPQPVRQPAPLPDQ
HYGVRPVRAPQPAE

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=223, Percent_Identity=50.6726457399103, Blast_Score=218, Evalue=3e-58,
Organism=Escherichia coli, GI1787738, Length=224, Percent_Identity=48.2142857142857, Blast_Score=207, Evalue=5e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 28252; Mature: 28121

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFDLNHVLFGWYPYLCLTVFLVGSLVRFDREQYTWKTGSSQLLRRKQLILGSVLFHVGV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHH
LAIFAGHFVGLLTPIAVFDALGVGHGFKQGLAIVAGGVAGTACFVGLSLLVHRRLYDPRI
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
RATSSFSDTAILLMLYAQLILGLSTIPVSLGHMDGHEMVKFMNWAQGVLTLQPGVADLVA
CCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHH
DVNPVFKLHLVLGMTIFLVFPFTRLVHVWSAPVWYLGRHGYQVVRTKKPQPVRQPAPLPD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCCCCCCCCC
QHYGVRPVRAPQPAE
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]