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The map label for this gene is nifM [H]

Identifier: 170748998

GI number: 170748998

Start: 2742725

End: 2743558

Strand: Reverse

Name: nifM [H]

Synonym: Mrad2831_2588

Alternate gene names: 170748998

Gene position: 2743558-2742725 (Counterclockwise)

Preceding gene: 170748999

Following gene: 170748997

Centisome position: 45.14

GC content: 71.7

Gene sequence:

>834_bases
ATGTCCGCCTGCTCCGTCCAAGCCCTGCCGAACGGCCCCAGGACCCCGATCCGGGTCAACGGGGTGACCATATCGCGGGC
GGTGATCTCGCGCGAGGTCCAGAACCACCCGGCCCCGACACCCGTCGGGGCCTGGAAGGCCGCCGCGCTCGCCCTCGTCC
TGCGCGAGGCCCTGTCCCAAGAAGTCAAGCGGCTCGATATCCGGGCCGAGCCCGCCGTGGACGCCGAGGGGCGGCGCGAG
ACCGACGAGGAAGCCTGCATGCGGGCGCTCGTCGAGCGCGAGGCGGTCGTGCCCGAGCCCACCGAGGAGGAGTGCCGCCG
CTACCATGCGCGCAATCTCGCCCGCTTCCGCTCGCCGGACATCTTCGAGGTCTCGCACATCCTGTTCTCGGCGCGGGCGG
ACGAAGCTTCGAGCTTCGCGCTGGCGCGGCTCAACGCTGAGACGGTCATCGCGATGCTGCGCACCGATCCGGACACGTTC
GCGGAGCTCGCGCGCGCGCACTCGGCCTGCCCCTCGGGCGAACTCGGCGGCAGCCTCGGCCAAGTCACGCAGGGTCAGAC
GACGCCTGAGTTCGAGGACGCCTTGAGGCGCATGACCCCGGGCCAGGTCTCCACCGAGCCGGTGGAGACTCGCTACGGCT
TTCACGTGATCCGCCTGTCCCGCCACATCGACGGCCGGACCCTGCCATTCGAGGCCGTTCGGACCTGCATCGCCGCGTAC
CTGTCGGAAGCCGTCCGGCGCCGGGCCGAGGCGCAGTACGTCGCGCGGCTCCTCGGGCAGGCCAGGGTCGAGGGCATCGA
GATCCCCGCGCCCGCCGCGCTCAACGTCCACTGA

Upstream 100 bases:

>100_bases
TGCGCCCGGTCCGGGCCCCCCAGCCCGCGGAGTAAGAGCCTTGGGTCCGAGGCAGTCCCGGGCCGCCATCCTACCCGTCC
AACACCCTGGGGACCGACCG

Downstream 100 bases:

>100_bases
GGAAACCGTCATGCTGCTCGGCGACATCATCGCGCGGCTCGACGACGAGGCCGTCGCCGACGAGACCCTCGTCCGGCTCG
GCGACCTCGTCCTGCTCGGC

Product: PpiC-type peptidyl-prolyl cis-trans isomerase

Products: peptidylproline (omega=0) [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSACSVQALPNGPRTPIRVNGVTISRAVISREVQNHPAPTPVGAWKAAALALVLREALSQEVKRLDIRAEPAVDAEGRRE
TDEEACMRALVEREAVVPEPTEEECRRYHARNLARFRSPDIFEVSHILFSARADEASSFALARLNAETVIAMLRTDPDTF
AELARAHSACPSGELGGSLGQVTQGQTTPEFEDALRRMTPGQVSTEPVETRYGFHVIRLSRHIDGRTLPFEAVRTCIAAY
LSEAVRRRAEAQYVARLLGQARVEGIEIPAPAALNVH

Sequences:

>Translated_277_residues
MSACSVQALPNGPRTPIRVNGVTISRAVISREVQNHPAPTPVGAWKAAALALVLREALSQEVKRLDIRAEPAVDAEGRRE
TDEEACMRALVEREAVVPEPTEEECRRYHARNLARFRSPDIFEVSHILFSARADEASSFALARLNAETVIAMLRTDPDTF
AELARAHSACPSGELGGSLGQVTQGQTTPEFEDALRRMTPGQVSTEPVETRYGFHVIRLSRHIDGRTLPFEAVRTCIAAY
LSEAVRRRAEAQYVARLLGQARVEGIEIPAPAALNVH
>Mature_276_residues
SACSVQALPNGPRTPIRVNGVTISRAVISREVQNHPAPTPVGAWKAAALALVLREALSQEVKRLDIRAEPAVDAEGRRET
DEEACMRALVEREAVVPEPTEEECRRYHARNLARFRSPDIFEVSHILFSARADEASSFALARLNAETVIAMLRTDPDTFA
ELARAHSACPSGELGGSLGQVTQGQTTPEFEDALRRMTPGQVSTEPVETRYGFHVIRLSRHIDGRTLPFEAVRTCIAAYL
SEAVRRRAEAQYVARLLGQARVEGIEIPAPAALNVH

Specific function: Required for the activation and stabilization of the iron-component (nifH) of nitrogenase. Probable PPIase [H]

COG id: COG0760

COG function: function code O; Parvulin-like peptidyl-prolyl isomerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PpiC domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014282
- InterPro:   IPR000297
- InterPro:   IPR023058
- InterPro:   IPR008880 [H]

Pfam domain/function: PF00639 Rotamase [H]

EC number: 5.2.1.8 [C]

Molecular weight: Translated: 30378; Mature: 30247

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS01096 PPIC_PPIASE_1 ; PS50198 PPIC_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSACSVQALPNGPRTPIRVNGVTISRAVISREVQNHPAPTPVGAWKAAALALVLREALSQ
CCCCCCCCCCCCCCCCEEECCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EVKRLDIRAEPAVDAEGRRETDEEACMRALVEREAVVPEPTEEECRRYHARNLARFRSPD
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
IFEVSHILFSARADEASSFALARLNAETVIAMLRTDPDTFAELARAHSACPSGELGGSLG
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHH
QVTQGQTTPEFEDALRRMTPGQVSTEPVETRYGFHVIRLSRHIDGRTLPFEAVRTCIAAY
CCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHH
LSEAVRRRAEAQYVARLLGQARVEGIEIPAPAALNVH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SACSVQALPNGPRTPIRVNGVTISRAVISREVQNHPAPTPVGAWKAAALALVLREALSQ
CCCCCCCCCCCCCCCEEECCEEHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
EVKRLDIRAEPAVDAEGRRETDEEACMRALVEREAVVPEPTEEECRRYHARNLARFRSPD
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
IFEVSHILFSARADEASSFALARLNAETVIAMLRTDPDTFAELARAHSACPSGELGGSLG
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHH
QVTQGQTTPEFEDALRRMTPGQVSTEPVETRYGFHVIRLSRHIDGRTLPFEAVRTCIAAY
CCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHH
LSEAVRRRAEAQYVARLLGQARVEGIEIPAPAALNVH
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Peptidoproline (omega=180) [C]

Specific reaction: Peptidoproline (omega=180) = peptidylproline (omega=0) [C]

General reaction: Rotation (Single bond) [C]

Inhibitor: cyclosporins [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885524 [H]