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The map label for this gene is murD [H]

Identifier: 170748777

GI number: 170748777

Start: 2496769

End: 2498133

Strand: Reverse

Name: murD [H]

Synonym: Mrad2831_2359

Alternate gene names: 170748777

Gene position: 2498133-2496769 (Counterclockwise)

Preceding gene: 170748778

Following gene: 170748775

Centisome position: 41.1

GC content: 72.6

Gene sequence:

>1365_bases
ATGACCCCCGTCACCGTCTTCCAGGACAAGACCGTCGCCCTGTTCGGACTCGGCGGCTCGGGGCTCGCCACGGCGCTGGC
GCTGAAGGCAGGTGGCGCGGCCGTGCAGGCCTGGGACGACAATGCCGACAGCCTCGCGCGCGCGCGGGAGGCCGGCATCG
AGACGCGGGACCTCCGGGAGGCCGACTGGTCCGGCTTCGCCGCCTTCGTGCTGAGCCCGGGCGTGCCGCTGACCCATCCC
GAGCCGCACTGGAGCGTCCGGCTCGCCCGGGACGCCGGCGTCGCGATCATCGGCGACATCGAGCTGTTCTGCCGGGAGCG
GCGGGCGCGGGCGCCGGGGGCGCCGTTCGTGGCGATCACCGGGACCAACGGCAAGTCGACCACCACGGCGCTGATCGCCC
ACGTGCTCGCGCAGGGGGGCCGCGACGTGCAGATGGGTGGCAATATCGGCACGGCGATCCTGTCGCTGGAGCCGCCGGCC
GCCGACCGCGTCCACGTGATCGAGATGTCGTCGTTCCAGATCGACCTGACGCCGAGCCTCGACCCGAGCGTCGGCGTGCT
GATGAACATCACGCCGGACCATCTCGACCGGCACGGCACGATGGAGCAGTACGCGGCCATCAAGGAGCGCCTGATCCAGG
GCGCCGGCCTCGCGGTGGTCGGCGTCGACGACGCCCCGAGCCGCGCCATCGCGGACCGCCGCGGCGCCGACCTCGTGCGC
GTCCACGTGCCCCACGGCGACGTTCTGTGCACCGACGCGCTGACGGTGCGCGACGGGATCGTCCGGGAGCCGGACGGGAC
GGCCCTCGCCGACGTCAACGGGATCGGCTCGCTCCGGGGGGCGCACAACTGGCAGAACGCCGCGGTGGCGGTCGCCGTGG
CGCGGGCCCTCGACCTGTCGCCGGAGCAGATCCAGGCCGGCCTGACCTCGTTCCCAGGCCTGCCGCACCGGATGGAGGAA
GTCGGGCGGCGCGGCCACGTGCTGTTCGTCAACGATTCGAAGGCGACGAACGCGGATTCCACCGAGAAGGCCCTCACCGC
CTTCCGGGACATCCACTGGATCCTCGGCGGCAAGGCCAAGGAGGGCGGGATCTTCCCGCTGGTGCCGTATTTCGAGCGGG
TCGCGCACGCCTACCTGATCGGCGCGGCGTCGGACGCCTTCGCGGCGACCCTGGAGGGCGCCGTGCCCTACACGCGCTGC
GAGACCCTCGAGAACGCCGTCCCGAAGGCCGCCGAGAACGCCGCCGCCTCGGGTGCACCGGAACCGGTGGTGCTCCTGTC
ACCGGCCTGCGCGTCCTACGACCAGTTCCGGAACTTCGAGATCCGCGGCAACCGCTTCCGCGAGCTGGTGCAGGCGCTGC
CCTGA

Upstream 100 bases:

>100_bases
GATCCCTGGAGCCCGCCTTCGGGGTCCGCCGACGCGGTCACCTCGGGATGACGGTCCGGTTCGGATCGGCCGGGTTCGGC
CGCCGCATCCAGAGTTCCGC

Downstream 100 bases:

>100_bases
GGCAGCGCCGGCCTCAGCGCGCCAGAACCTTGGCGGCGTCGCGGGTGGTGTCGACCGCCTGCGAGACGTTGGCCACCGAC
TGGACGATCGACCCGATATT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MTPVTVFQDKTVALFGLGGSGLATALALKAGGAAVQAWDDNADSLARAREAGIETRDLREADWSGFAAFVLSPGVPLTHP
EPHWSVRLARDAGVAIIGDIELFCRERRARAPGAPFVAITGTNGKSTTTALIAHVLAQGGRDVQMGGNIGTAILSLEPPA
ADRVHVIEMSSFQIDLTPSLDPSVGVLMNITPDHLDRHGTMEQYAAIKERLIQGAGLAVVGVDDAPSRAIADRRGADLVR
VHVPHGDVLCTDALTVRDGIVREPDGTALADVNGIGSLRGAHNWQNAAVAVAVARALDLSPEQIQAGLTSFPGLPHRMEE
VGRRGHVLFVNDSKATNADSTEKALTAFRDIHWILGGKAKEGGIFPLVPYFERVAHAYLIGAASDAFAATLEGAVPYTRC
ETLENAVPKAAENAAASGAPEPVVLLSPACASYDQFRNFEIRGNRFRELVQALP

Sequences:

>Translated_454_residues
MTPVTVFQDKTVALFGLGGSGLATALALKAGGAAVQAWDDNADSLARAREAGIETRDLREADWSGFAAFVLSPGVPLTHP
EPHWSVRLARDAGVAIIGDIELFCRERRARAPGAPFVAITGTNGKSTTTALIAHVLAQGGRDVQMGGNIGTAILSLEPPA
ADRVHVIEMSSFQIDLTPSLDPSVGVLMNITPDHLDRHGTMEQYAAIKERLIQGAGLAVVGVDDAPSRAIADRRGADLVR
VHVPHGDVLCTDALTVRDGIVREPDGTALADVNGIGSLRGAHNWQNAAVAVAVARALDLSPEQIQAGLTSFPGLPHRMEE
VGRRGHVLFVNDSKATNADSTEKALTAFRDIHWILGGKAKEGGIFPLVPYFERVAHAYLIGAASDAFAATLEGAVPYTRC
ETLENAVPKAAENAAASGAPEPVVLLSPACASYDQFRNFEIRGNRFRELVQALP
>Mature_453_residues
TPVTVFQDKTVALFGLGGSGLATALALKAGGAAVQAWDDNADSLARAREAGIETRDLREADWSGFAAFVLSPGVPLTHPE
PHWSVRLARDAGVAIIGDIELFCRERRARAPGAPFVAITGTNGKSTTTALIAHVLAQGGRDVQMGGNIGTAILSLEPPAA
DRVHVIEMSSFQIDLTPSLDPSVGVLMNITPDHLDRHGTMEQYAAIKERLIQGAGLAVVGVDDAPSRAIADRRGADLVRV
HVPHGDVLCTDALTVRDGIVREPDGTALADVNGIGSLRGAHNWQNAAVAVAVARALDLSPEQIQAGLTSFPGLPHRMEEV
GRRGHVLFVNDSKATNADSTEKALTAFRDIHWILGGKAKEGGIFPLVPYFERVAHAYLIGAASDAFAATLEGAVPYTRCE
TLENAVPKAAENAAASGAPEPVVLLSPACASYDQFRNFEIRGNRFRELVQALP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=461, Percent_Identity=38.177874186551, Blast_Score=215, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 47918; Mature: 47787

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVTVFQDKTVALFGLGGSGLATALALKAGGAAVQAWDDNADSLARAREAGIETRDLRE
CCCCEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
ADWSGFAAFVLSPGVPLTHPEPHWSVRLARDAGVAIIGDIELFCRERRARAPGAPFVAIT
CCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHVLAQGGRDVQMGGNIGTAILSLEPPAADRVHVIEMSSFQIDLTPSL
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSVGVLMNITPDHLDRHGTMEQYAAIKERLIQGAGLAVVGVDDAPSRAIADRRGADLVR
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCEEE
VHVPHGDVLCTDALTVRDGIVREPDGTALADVNGIGSLRGAHNWQNAAVAVAVARALDLS
EECCCCCEEEECCEECCCCCEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
PEQIQAGLTSFPGLPHRMEEVGRRGHVLFVNDSKATNADSTEKALTAFRDIHWILGGKAK
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCC
EGGIFPLVPYFERVAHAYLIGAASDAFAATLEGAVPYTRCETLENAVPKAAENAAASGAP
CCCCCCCHHHHHHHHHHHEEECCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCCCCC
EPVVLLSPACASYDQFRNFEIRGNRFRELVQALP
CCEEEECCCHHCHHHHCCEEECCHHHHHHHHHCC
>Mature Secondary Structure 
TPVTVFQDKTVALFGLGGSGLATALALKAGGAAVQAWDDNADSLARAREAGIETRDLRE
CCCEEECCCEEEEEECCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHCCCHHHHHH
ADWSGFAAFVLSPGVPLTHPEPHWSVRLARDAGVAIIGDIELFCRERRARAPGAPFVAIT
CCCCCEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEECHHHHHHHHHCCCCCCCEEEEE
GTNGKSTTTALIAHVLAQGGRDVQMGGNIGTAILSLEPPAADRVHVIEMSSFQIDLTPSL
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCCCCCEEEEEECCEEEEECCCC
DPSVGVLMNITPDHLDRHGTMEQYAAIKERLIQGAGLAVVGVDDAPSRAIADRRGADLVR
CCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCCEEE
VHVPHGDVLCTDALTVRDGIVREPDGTALADVNGIGSLRGAHNWQNAAVAVAVARALDLS
EECCCCCEEEECCEECCCCCEECCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
PEQIQAGLTSFPGLPHRMEEVGRRGHVLFVNDSKATNADSTEKALTAFRDIHWILGGKAK
HHHHHHHHHHCCCCCHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHEECCCCC
EGGIFPLVPYFERVAHAYLIGAASDAFAATLEGAVPYTRCETLENAVPKAAENAAASGAP
CCCCCCCHHHHHHHHHHHEEECCCCHHHHHHCCCCCHHHHHHHHHHCCHHHHHCCCCCCC
EPVVLLSPACASYDQFRNFEIRGNRFRELVQALP
CCEEEECCCHHCHHHHCCEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA