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The map label for this gene is nylB [H]

Identifier: 170748087

GI number: 170748087

Start: 1782525

End: 1783757

Strand: Reverse

Name: nylB [H]

Synonym: Mrad2831_1669

Alternate gene names: 170748087

Gene position: 1783757-1782525 (Counterclockwise)

Preceding gene: 170748090

Following gene: 170748085

Centisome position: 29.35

GC content: 72.83

Gene sequence:

>1233_bases
ATGACCGCGTCCATGGCGATCCCGGATTCCCCGGCCCGCACACGCCGGGGCCGCCGCTGGGGCGCGATCGCGGCGGCGGG
CGGCGCGGCCGTCGTCGGCTGCGCCTGGACCGCCGCCGCGCTGATCAACGTCCCCGACCCCGTCACCCTGGCGGCGCTCG
CGGTCATCGAGCCGTCGTCGGTGGGCACGTGGTTCCCGAGCCGGATCGTCCAGGCCCCGACCCGGGCGAGCGACCTGCCG
GTGCGGCCGCGCCCGATCCTCGACCGCGTCCCCTGGAAGGGGAAGACGGTCCCGCTGATGACGGTGCTGGGCGCGACCCA
CACCAACGCGTTCCTGGTGCTGCAGGACGGCGTCCTGATCCACGAATGGCAGCGCGCGGGCACCGGACCGGAGACGCTGT
TTCCCTCGTGGTCGGTGGCGAAGTCCGTCGTCTCGCTGCTGGTCGGCGCCGCGGTGGCGCGGGGCCAGCTCGCCGAGACC
GACCGGGTCTCCACGCTCCTGCCCGAGTTGCGGAACGGCGCGGTGTTCGGTCAGATCACCGTGCGCAACCTCCTCGACAT
GGCGAGCGGGATCGCGGTGCCGGAGAACTACGATCCCCGGCACCCCCTGACCGGCACCGCCGGGATGTACCTGACGCGCG
ACCTCACGGCCTTCGTGCGCGACAACGCCCACCTGGCTTTCAAGCCCGGCACCAAGGGCCGCTACCGCAGCATCGACACC
GAGCTGCTCGGCCTGATCCTCGCCCGGGTCGAGGGCAAGCCGCTGGCCGACATCCTCTCGGAGCGGATCTGGAAGCCGAT
GGGCGCCCAGGCCGACGCCACCTGGAACCTCGACCGGCCCGGCGGCATCGAGAAGGCGTTCTGCTGCATCAACGCGACGG
CCCGCGACTTCGCCCGCCTCGGCCTGCTGGTGGCCGACCAGGGCCGGTCGGGCGAACACCGCATCATCCCGGGCCGCTGG
ATCGAGCGGATCATGACCCCGGCCCGGCGCGACGTCGACGGCTGGCAGTACTCGGCCCAGTGGTGGCACGCGCCGGGGGG
CGAGGACGACGACATCTCGGCGATCGGCGTCTACGGCCAGTACATCTACGTCAATCGCGCGACCGGCACGGTGATCGTGA
AGCTCAGCGACCACGGCGCCGAGCAGGACGAGGTCGACACGCTGGCGGTGATGCAGGCCATCGCGGGCGATCTCGCCGCC
GGTCGCCCCGCCGCCGCCCGCAAGGACCCGTGA

Upstream 100 bases:

>100_bases
TGGGGCTTTCGTTAATGCCTCCTGATCATGGTGAGCGCGCGCCGTCCCGCCCCGGGGCCGGCCGCGCGACCGCGACGATC
CAGAGCGACACAGACCCAGC

Downstream 100 bases:

>100_bases
AGGACCCGTGAAGGACCCGTGAGCGGCGGGGGCCGCGGCCCGAGTCCCGACCGGGCCGCGCTCAGGCCTCTGTCGGACCG
GCCCCCGGCACGGAGGATCG

Product: beta-lactamase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EII [H]

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MTASMAIPDSPARTRRGRRWGAIAAAGGAAVVGCAWTAAALINVPDPVTLAALAVIEPSSVGTWFPSRIVQAPTRASDLP
VRPRPILDRVPWKGKTVPLMTVLGATHTNAFLVLQDGVLIHEWQRAGTGPETLFPSWSVAKSVVSLLVGAAVARGQLAET
DRVSTLLPELRNGAVFGQITVRNLLDMASGIAVPENYDPRHPLTGTAGMYLTRDLTAFVRDNAHLAFKPGTKGRYRSIDT
ELLGLILARVEGKPLADILSERIWKPMGAQADATWNLDRPGGIEKAFCCINATARDFARLGLLVADQGRSGEHRIIPGRW
IERIMTPARRDVDGWQYSAQWWHAPGGEDDDISAIGVYGQYIYVNRATGTVIVKLSDHGAEQDEVDTLAVMQAIAGDLAA
GRPAAARKDP

Sequences:

>Translated_410_residues
MTASMAIPDSPARTRRGRRWGAIAAAGGAAVVGCAWTAAALINVPDPVTLAALAVIEPSSVGTWFPSRIVQAPTRASDLP
VRPRPILDRVPWKGKTVPLMTVLGATHTNAFLVLQDGVLIHEWQRAGTGPETLFPSWSVAKSVVSLLVGAAVARGQLAET
DRVSTLLPELRNGAVFGQITVRNLLDMASGIAVPENYDPRHPLTGTAGMYLTRDLTAFVRDNAHLAFKPGTKGRYRSIDT
ELLGLILARVEGKPLADILSERIWKPMGAQADATWNLDRPGGIEKAFCCINATARDFARLGLLVADQGRSGEHRIIPGRW
IERIMTPARRDVDGWQYSAQWWHAPGGEDDDISAIGVYGQYIYVNRATGTVIVKLSDHGAEQDEVDTLAVMQAIAGDLAA
GRPAAARKDP
>Mature_409_residues
TASMAIPDSPARTRRGRRWGAIAAAGGAAVVGCAWTAAALINVPDPVTLAALAVIEPSSVGTWFPSRIVQAPTRASDLPV
RPRPILDRVPWKGKTVPLMTVLGATHTNAFLVLQDGVLIHEWQRAGTGPETLFPSWSVAKSVVSLLVGAAVARGQLAETD
RVSTLLPELRNGAVFGQITVRNLLDMASGIAVPENYDPRHPLTGTAGMYLTRDLTAFVRDNAHLAFKPGTKGRYRSIDTE
LLGLILARVEGKPLADILSERIWKPMGAQADATWNLDRPGGIEKAFCCINATARDFARLGLLVADQGRSGEHRIIPGRWI
ERIMTPARRDVDGWQYSAQWWHAPGGEDDDISAIGVYGQYIYVNRATGTVIVKLSDHGAEQDEVDTLAVMQAIAGDLAAG
RPAAARKDP

Specific function: Unknown

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: =3.5.1.46 [H]

Molecular weight: Translated: 44078; Mature: 43947

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASMAIPDSPARTRRGRRWGAIAAAGGAAVVGCAWTAAALINVPDPVTLAALAVIEPSS
CCCCCCCCCCCHHHHCCCCCCEEEECCCCEEEHHHHHHHHEECCCCCHHHHEEEEECCCC
VGTWFPSRIVQAPTRASDLPVRPRPILDRVPWKGKTVPLMTVLGATHTNAFLVLQDGVLI
CCCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCEEHEEEECCCCCCEEEEEECCEEE
HEWQRAGTGPETLFPSWSVAKSVVSLLVGAAVARGQLAETDRVSTLLPELRNGAVFGQIT
EEHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEH
VRNLLDMASGIAVPENYDPRHPLTGTAGMYLTRDLTAFVRDNAHLAFKPGTKGRYRSIDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
ELLGLILARVEGKPLADILSERIWKPMGAQADATWNLDRPGGIEKAFCCINATARDFARL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEEEECCHHHHHHHH
GLLVADQGRSGEHRIIPGRWIERIMTPARRDVDGWQYSAQWWHAPGGEDDDISAIGVYGQ
CEEEECCCCCCCCEECCHHHHHHHHCHHHCCCCCCEECCEEEECCCCCCCCCEEEEEEEE
YIYVNRATGTVIVKLSDHGAEQDEVDTLAVMQAIAGDLAAGRPAAARKDP
EEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TASMAIPDSPARTRRGRRWGAIAAAGGAAVVGCAWTAAALINVPDPVTLAALAVIEPSS
CCCCCCCCCCHHHHCCCCCCEEEECCCCEEEHHHHHHHHEECCCCCHHHHEEEEECCCC
VGTWFPSRIVQAPTRASDLPVRPRPILDRVPWKGKTVPLMTVLGATHTNAFLVLQDGVLI
CCCCCHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCEEHEEEECCCCCCEEEEEECCEEE
HEWQRAGTGPETLFPSWSVAKSVVSLLVGAAVARGQLAETDRVSTLLPELRNGAVFGQIT
EEHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCEEEEEEH
VRNLLDMASGIAVPENYDPRHPLTGTAGMYLTRDLTAFVRDNAHLAFKPGTKGRYRSIDT
HHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHH
ELLGLILARVEGKPLADILSERIWKPMGAQADATWNLDRPGGIEKAFCCINATARDFARL
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCEEEEEECCHHHHHHHH
GLLVADQGRSGEHRIIPGRWIERIMTPARRDVDGWQYSAQWWHAPGGEDDDISAIGVYGQ
CEEEECCCCCCCCEECCHHHHHHHHCHHHCCCCCCEECCEEEECCCCCCCCCEEEEEEEE
YIYVNRATGTVIVKLSDHGAEQDEVDTLAVMQAIAGDLAAGRPAAARKDP
EEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6646204; 6389532; 2512123 [H]