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The map label for this gene is rutD

Identifier: 170747999

GI number: 170747999

Start: 1670601

End: 1671380

Strand: Reverse

Name: rutD

Synonym: Mrad2831_1581

Alternate gene names: 170747999

Gene position: 1671380-1670601 (Counterclockwise)

Preceding gene: 170748000

Following gene: 170747994

Centisome position: 27.5

GC content: 77.44

Gene sequence:

>780_bases
ATGGGCCCGCTCCACCACGCTGTGGCGGGCCCGGCGGGCGGTCGGCCGGTGCTGCTCTCGCCGGGGCTCGGCGGCGCGGC
GGGCTACTTCGCGCCGCAGATGGCGGCCCTGACCGCGCGGTTCCGGGTCGTCACCTACGACCATCGCGGCACCGGGCGCT
CGCCCGGCCGGCTCGCGCCGGACCACGACGTCCCCGCCATGGCGCGGGACGCGCGCGCGGTGCTGGACGCGGCCGGGATC
GAGCGGGCCGACGTGGTCGGCCACGCGCTGGGCGGTCTGATCGCCCTCCAGATGGCTTTGGACGCGCCCGACCGGGTCGG
GCGGGTCGTGGTGATCAACGGCTGGGACGCCCTGGACCCGGCCACGCGCCGCTGCTTCGCGGCCCGCCGGGCGATCCTGG
CCGGGGGCGGACCGGAGGCCTTCGTGCGGGCGCAGGCGATCTTCCTGTACCCGGCGGCGTGGCTCTCCGCGCAGGCCGAG
CGGGTCGAGGCCGACGAGGCGCAGGCCCTGGCGCATTTTCCCGGCGCCGAGACCGTGCTGACGCGCATCGCCGCCCTGGA
GGGCTTCGCCATCGCCGACCGCCTCGGAGCGATCGGCCACGAGACCCTGGTGATGGCCGCCCGGGACGACGTGCTGGTGC
CCTACACCCGGTCCGAGCGGCTGGCGGCGGCGCTGCCCAACGCCCGGCTCGCCCTCGCCCCGGAGGGCGGCCACGCCCAC
AGCGTCACCCGGCCCGAGGCCTTCAACCGGGCGCTCCTGGACTTCCTGGACCGGGACTGA

Upstream 100 bases:

>100_bases
ATTTCCCGGGCGAGAAGCCCGCGCGCTACTGCATCCAGTGCGGCCTCGTGAAGCCCGAGGCCCGCGTGGAGATCGCCGCG
ATCGCCCATATCGGCCGCTG

Downstream 100 bases:

>100_bases
GAGCCCGCTACGCCCGCAGCGCGATCATCGGCAGCACCACCGCGTCGAGGGCGGCGGCCAGCACCGGGAGCAGGGCCGGC
CAGCGCAGGGTCGCCGCGGT

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MGPLHHAVAGPAGGRPVLLSPGLGGAAGYFAPQMAALTARFRVVTYDHRGTGRSPGRLAPDHDVPAMARDARAVLDAAGI
ERADVVGHALGGLIALQMALDAPDRVGRVVVINGWDALDPATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLSAQAE
RVEADEAQALAHFPGAETVLTRIAALEGFAIADRLGAIGHETLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAH
SVTRPEAFNRALLDFLDRD

Sequences:

>Translated_259_residues
MGPLHHAVAGPAGGRPVLLSPGLGGAAGYFAPQMAALTARFRVVTYDHRGTGRSPGRLAPDHDVPAMARDARAVLDAAGI
ERADVVGHALGGLIALQMALDAPDRVGRVVVINGWDALDPATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLSAQAE
RVEADEAQALAHFPGAETVLTRIAALEGFAIADRLGAIGHETLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAH
SVTRPEAFNRALLDFLDRD
>Mature_258_residues
GPLHHAVAGPAGGRPVLLSPGLGGAAGYFAPQMAALTARFRVVTYDHRGTGRSPGRLAPDHDVPAMARDARAVLDAAGIE
RADVVGHALGGLIALQMALDAPDRVGRVVVINGWDALDPATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLSAQAER
VEADEAQALAHFPGAETVLTRIAALEGFAIADRLGAIGHETLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAHS
VTRPEAFNRALLDFLDRD

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=233, Percent_Identity=45.9227467811159, Blast_Score=208, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_METRJ (B1M5I5)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001754259.1
- ProteinModelPortal:   B1M5I5
- GeneID:   6137609
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1581
- HOGENOM:   HBG748003
- OMA:   FLYPAAW
- ProtClustDB:   CLSK2330819
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR019913
- PRINTS:   PR00111
- PRINTS:   PR00412
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 27128; Mature: 26997

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPLHHAVAGPAGGRPVLLSPGLGGAAGYFAPQMAALTARFRVVTYDHRGTGRSPGRLAP
CCCCCCHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCC
DHDVPAMARDARAVLDAAGIERADVVGHALGGLIALQMALDAPDRVGRVVVINGWDALDP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCH
ATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLSAQAERVEADEAQALAHFPGAETVL
HHHHHHHHHHHHHCCCCHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TRIAALEGFAIADRLGAIGHETLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAH
HHHHHHCCHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHHCCCCEEEEECCCCCCC
SVTRPEAFNRALLDFLDRD
CCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GPLHHAVAGPAGGRPVLLSPGLGGAAGYFAPQMAALTARFRVVTYDHRGTGRSPGRLAP
CCCCCHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCC
DHDVPAMARDARAVLDAAGIERADVVGHALGGLIALQMALDAPDRVGRVVVINGWDALDP
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECCCCCCH
ATRRCFAARRAILAGGGPEAFVRAQAIFLYPAAWLSAQAERVEADEAQALAHFPGAETVL
HHHHHHHHHHHHHCCCCHHHHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TRIAALEGFAIADRLGAIGHETLVMAARDDVLVPYTRSERLAAALPNARLALAPEGGHAH
HHHHHHCCHHHHHHHCCCCCCEEEEEECCCEEECCCCCCHHHHHCCCCEEEEECCCCCCC
SVTRPEAFNRALLDFLDRD
CCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA