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The map label for this gene is rfbE [H]

Identifier: 170747742

GI number: 170747742

Start: 1378474

End: 1379538

Strand: Reverse

Name: rfbE [H]

Synonym: Mrad2831_1321

Alternate gene names: 170747742

Gene position: 1379538-1378474 (Counterclockwise)

Preceding gene: 170747743

Following gene: 170747741

Centisome position: 22.7

GC content: 74.27

Gene sequence:

>1065_bases
GTGAGCGGCCGGCCGATCCTCGTCACCGGGGGCGCCGGGTTCATCGGTGCCAACCTGTCCGACAGCCTCGCGGCCGACGG
CCACGCGGTGATCGTCTACGACATCCTCTCGCGCCCCGGCGTCGAGGCCAACCTCGCGTGGCTGCGGCAGCGCCACGGCG
CCCGGATCATCCCGATCGTCGCCGACATCCGCGACATCCAGGAGCTCGGCCGCGCGGTGGCGGAGGCCGGCGCGGTGTTC
CACTTCGCCGCCCAGGTGGCGGTGACGACGAGCCTCGTCGCGCCGCGGGCCGACATGGAGATCAACCTCCTCGGCACCGT
GAACCTGCTGGAGGCCCTGCGGGCGCAGGCGCGGCCGGTGCCGCTCCTCTACGCCTCGACCAACAAGGTCTACGGCGACC
TGCCCGACATCGCCCTGGAGGCGGTCGGCGACGCCTATCTGCCGGCCGATCCGGACCTGCGCCGGCGCGGCATCGGCGAG
ACCCGGCCCCTCGACTTCCACACGCCCTACGGCTGCTCCAAGGGAGCGGCGGACCAGTACGTCCTCGACTACGCCCGCAG
CTTCGGCGTCCCGGCGGCGGTGATGCGGATGAGCTGCATCTACGGTCCGCGCCAGATGGGCAACGAGGACCAGGGCTGGG
TCGCCCACTTCCTGATCCGGGCGCTCGCCGGCGAGGCGGTCACGCTCTACGGCGACGGACGGCAGGTGCGCGACATCCTG
CACGTGCGCGACGTGGTGGCGGTCTACCGGGCGGCGCTCGCCCGCATCGACGACGCGGCGGGCCACGCCTTCAACGTCGG
CGGCGGCCCCGGCAACGCCGTGAGCCTGCTGCAGCTCCTCGACCATGTCGGCACCCTGCTGGGGCGCCCGGTATCGCGCA
GCTTCCAGGCGTGGCGCCCGGGCGATCAGCGCTACTACGTCTCGGACACGCGCCGGGCCGTCGCGCGACTCGGCCTCGCC
CCCTTCACGGGCTGGCGCGACGGCGTCGCCGACCTCGCCGGCTGGCTCGCCGCCGCGCGCGCGTCGTCGGATGGCGCGGC
ACCGATCGCGGGGGCGGCCGCATGA

Upstream 100 bases:

>100_bases
CCGAGGGGCTCGCCGAACTCGCCGCCTGGGTGGCCGAGCAGCAGGCCGTCGACCGCGTCGCCGAGGCGCGGCGCGAACTC
GAAGCGCGCGGTCTCGTCGC

Downstream 100 bases:

>100_bases
GAGTCGCGCTGGTCAACCCGCCCTGGGACTACCGCAACAGCATCTATTTCGGCTGCCGCGAGCCGCACCTGCCGCTCGAA
CTCGGCTACTCCGCCGCGCT

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: CDP-tyvelose 2-epimerase [H]

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSGRPILVTGGAGFIGANLSDSLAADGHAVIVYDILSRPGVEANLAWLRQRHGARIIPIVADIRDIQELGRAVAEAGAVF
HFAAQVAVTTSLVAPRADMEINLLGTVNLLEALRAQARPVPLLYASTNKVYGDLPDIALEAVGDAYLPADPDLRRRGIGE
TRPLDFHTPYGCSKGAADQYVLDYARSFGVPAAVMRMSCIYGPRQMGNEDQGWVAHFLIRALAGEAVTLYGDGRQVRDIL
HVRDVVAVYRAALARIDDAAGHAFNVGGGPGNAVSLLQLLDHVGTLLGRPVSRSFQAWRPGDQRYYVSDTRRAVARLGLA
PFTGWRDGVADLAGWLAAARASSDGAAPIAGAAA

Sequences:

>Translated_354_residues
MSGRPILVTGGAGFIGANLSDSLAADGHAVIVYDILSRPGVEANLAWLRQRHGARIIPIVADIRDIQELGRAVAEAGAVF
HFAAQVAVTTSLVAPRADMEINLLGTVNLLEALRAQARPVPLLYASTNKVYGDLPDIALEAVGDAYLPADPDLRRRGIGE
TRPLDFHTPYGCSKGAADQYVLDYARSFGVPAAVMRMSCIYGPRQMGNEDQGWVAHFLIRALAGEAVTLYGDGRQVRDIL
HVRDVVAVYRAALARIDDAAGHAFNVGGGPGNAVSLLQLLDHVGTLLGRPVSRSFQAWRPGDQRYYVSDTRRAVARLGLA
PFTGWRDGVADLAGWLAAARASSDGAAPIAGAAA
>Mature_353_residues
SGRPILVTGGAGFIGANLSDSLAADGHAVIVYDILSRPGVEANLAWLRQRHGARIIPIVADIRDIQELGRAVAEAGAVFH
FAAQVAVTTSLVAPRADMEINLLGTVNLLEALRAQARPVPLLYASTNKVYGDLPDIALEAVGDAYLPADPDLRRRGIGET
RPLDFHTPYGCSKGAADQYVLDYARSFGVPAAVMRMSCIYGPRQMGNEDQGWVAHFLIRALAGEAVTLYGDGRQVRDILH
VRDVVAVYRAALARIDDAAGHAFNVGGGPGNAVSLLQLLDHVGTLLGRPVSRSFQAWRPGDQRYYVSDTRRAVARLGLAP
FTGWRDGVADLAGWLAAARASSDGAAPIAGAAA

Specific function: Catalyzes the isomeration of CDP-paratose to CDP- tyvelose [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=342, Percent_Identity=27.4853801169591, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI42516563, Length=326, Percent_Identity=28.5276073619632, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=358, Percent_Identity=26.8156424581006, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI56118217, Length=358, Percent_Identity=26.8156424581006, Blast_Score=79, Evalue=7e-15,
Organism=Homo sapiens, GI189083684, Length=358, Percent_Identity=26.8156424581006, Blast_Score=79, Evalue=7e-15,
Organism=Escherichia coli, GI48994969, Length=362, Percent_Identity=29.8342541436464, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788353, Length=348, Percent_Identity=30.1724137931034, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786974, Length=334, Percent_Identity=27.5449101796407, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=337, Percent_Identity=28.1899109792285, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=337, Percent_Identity=26.4094955489614, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=26.4880952380952, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71982035, Length=358, Percent_Identity=25.6983240223464, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982038, Length=360, Percent_Identity=25.2777777777778, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=302, Percent_Identity=28.476821192053, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI21356223, Length=248, Percent_Identity=30.6451612903226, Blast_Score=93, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =5.1.3.10 [H]

Molecular weight: Translated: 37521; Mature: 37390

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGRPILVTGGAGFIGANLSDSLAADGHAVIVYDILSRPGVEANLAWLRQRHGARIIPIV
CCCCCEEEECCCCEEECCCCCHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHCCCEEEEHH
ADIRDIQELGRAVAEAGAVFHFAAQVAVTTSLVAPRADMEINLLGTVNLLEALRAQARPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCC
PLLYASTNKVYGDLPDIALEAVGDAYLPADPDLRRRGIGETRPLDFHTPYGCSKGAADQY
EEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
VLDYARSFGVPAAVMRMSCIYGPRQMGNEDQGWVAHFLIRALAGEAVTLYGDGRQVRDIL
HHHHHHHCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHH
HVRDVVAVYRAALARIDDAAGHAFNVGGGPGNAVSLLQLLDHVGTLLGRPVSRSFQAWRP
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCHHHCCC
GDQRYYVSDTRRAVARLGLAPFTGWRDGVADLAGWLAAARASSDGAAPIAGAAA
CCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGRPILVTGGAGFIGANLSDSLAADGHAVIVYDILSRPGVEANLAWLRQRHGARIIPIV
CCCCEEEECCCCEEECCCCCHHCCCCCEEEEEEHHCCCCCCHHHHHHHHHCCCEEEEHH
ADIRDIQELGRAVAEAGAVFHFAAQVAVTTSLVAPRADMEINLLGTVNLLEALRAQARPV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCC
PLLYASTNKVYGDLPDIALEAVGDAYLPADPDLRRRGIGETRPLDFHTPYGCSKGAADQY
EEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCHHH
VLDYARSFGVPAAVMRMSCIYGPRQMGNEDQGWVAHFLIRALAGEAVTLYGDGRQVRDIL
HHHHHHHCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHCCCEEEEEECCHHHHHHH
HVRDVVAVYRAALARIDDAAGHAFNVGGGPGNAVSLLQLLDHVGTLLGRPVSRSFQAWRP
HHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCHHHCCC
GDQRYYVSDTRRAVARLGLAPFTGWRDGVADLAGWLAAARASSDGAAPIAGAAA
CCCEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2793833; 11677608; 12644504; 12642575 [H]