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The map label for this gene is gno [H]

Identifier: 170747626

GI number: 170747626

Start: 1257486

End: 1258223

Strand: Reverse

Name: gno [H]

Synonym: Mrad2831_1196

Alternate gene names: 170747626

Gene position: 1258223-1257486 (Counterclockwise)

Preceding gene: 170747627

Following gene: 170747625

Centisome position: 20.7

GC content: 76.56

Gene sequence:

>738_bases
ATGTCGCAGCCGATCGCGGAGCCGGGGGGGCACGTCCTCGTCGTCGGGGCCAGCAGCGGCATCGGCCGCGCCGTCGCCGA
GCGCCTCCGCGCGGGCGGGGCGCGGATCACCGCCCTCGCCCGGCGGGCGGACCGGCTCGCCGAGCTGGAGGCTCTCGGCT
GCGCCGCCGTCGCGGCCGACGTCACCGACCTGCCGGCGATCGGCGGCGTGCTGCAGGCGGCGGTCGCCGAGCGGGGGCTC
CTGACCGGGCTCGTGTACTGCGCGGGCGTCCAGGTCATCAAGCCGATGCGCAGCCTCGGCGTGGAGGAGGTCGAGCGCCT
CTACCGCACCAACCTCGTCGCGCCGACCCTGTTCGCCGCCGCCTTCGGCAGCGCCCGGGTCTCGACCCGGGACGCGGTGT
TCTGCGCGGTCTCGTCCGTGGCGGCCCAGCGGCCCGAGCCGGCGATCGTGCCCTACGCCGCCTCCAAGGCGGGGCTCGAC
GCCCTGATCAAGGGGCTCGCCCGGGAACTCGCCCCCCGGCGCGCCGTCGGCGTCGCCCCCGGCTGGCTCGACACCGAGAT
GACCCAGGCCCATGCCCGCCTGTACGGCGCGGAGTTCAAGGAGGCGCTGGAGAAGCGCAGCCCGGCGGGCGCCGCGACCG
TCGAATCCGTGGTCGACGCCATCGCGTTCCTGATGTCGCCCCAGGCCCGGCACGTCACCGGCGAGATCCTGCGCGTCGAC
GGCGGGGCCGTGCTGTGA

Upstream 100 bases:

>100_bases
GACACGCTGGGCCTCGAGGTGGATCCGCAGCGGATCGCCGGGGCGGCGACCACCGACGACCTGATCGCGCTGGCCGGCGC
GAAGCTGCAGGCCTGAGCCG

Downstream 100 bases:

>100_bases
CCGGGCCCGGGCAGCGGCTCGCGCTGATCGGCGGCGGCGGATTCTCCAAGGAGATCGCCGAGATCGCGCGCGCCCTCGGC
CACACGGTCGAGGATACCTA

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: 5-keto-D-gluconate 5-reductase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSQPIAEPGGHVLVVGASSGIGRAVAERLRAGGARITALARRADRLAELEALGCAAVAADVTDLPAIGGVLQAAVAERGL
LTGLVYCAGVQVIKPMRSLGVEEVERLYRTNLVAPTLFAAAFGSARVSTRDAVFCAVSSVAAQRPEPAIVPYAASKAGLD
ALIKGLARELAPRRAVGVAPGWLDTEMTQAHARLYGAEFKEALEKRSPAGAATVESVVDAIAFLMSPQARHVTGEILRVD
GGAVL

Sequences:

>Translated_245_residues
MSQPIAEPGGHVLVVGASSGIGRAVAERLRAGGARITALARRADRLAELEALGCAAVAADVTDLPAIGGVLQAAVAERGL
LTGLVYCAGVQVIKPMRSLGVEEVERLYRTNLVAPTLFAAAFGSARVSTRDAVFCAVSSVAAQRPEPAIVPYAASKAGLD
ALIKGLARELAPRRAVGVAPGWLDTEMTQAHARLYGAEFKEALEKRSPAGAATVESVVDAIAFLMSPQARHVTGEILRVD
GGAVL
>Mature_244_residues
SQPIAEPGGHVLVVGASSGIGRAVAERLRAGGARITALARRADRLAELEALGCAAVAADVTDLPAIGGVLQAAVAERGLL
TGLVYCAGVQVIKPMRSLGVEEVERLYRTNLVAPTLFAAAFGSARVSTRDAVFCAVSSVAAQRPEPAIVPYAASKAGLDA
LIKGLARELAPRRAVGVAPGWLDTEMTQAHARLYGAEFKEALEKRSPAGAATVESVVDAIAFLMSPQARHVTGEILRVDG
GAVL

Specific function: Involved in the non-phosphorylative, ketogenic oxidation of glucose and oxidizes gluconate to 5-ketogluconate. Dependent on NADP, almost inactive with NAD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=33.7349397590361, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI5031737, Length=243, Percent_Identity=31.2757201646091, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4503817, Length=181, Percent_Identity=30.939226519337, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI40254992, Length=246, Percent_Identity=29.2682926829268, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI19923817, Length=247, Percent_Identity=25.1012145748988, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787335, Length=239, Percent_Identity=33.0543933054393, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1790717, Length=254, Percent_Identity=29.5275590551181, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI2367365, Length=240, Percent_Identity=31.6666666666667, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1786812, Length=251, Percent_Identity=30.6772908366534, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=241, Percent_Identity=30.7053941908714, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI71994600, Length=245, Percent_Identity=27.7551020408163, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=245, Percent_Identity=28.9795918367347, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=245, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=239, Percent_Identity=28.4518828451883, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI193204405, Length=262, Percent_Identity=28.2442748091603, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24643142, Length=243, Percent_Identity=32.0987654320988, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI23397609, Length=244, Percent_Identity=33.1967213114754, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21357041, Length=244, Percent_Identity=31.5573770491803, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=244, Percent_Identity=31.5573770491803, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24644339, Length=251, Percent_Identity=31.8725099601594, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24639444, Length=244, Percent_Identity=27.8688524590164, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.1.1.69 [H]

Molecular weight: Translated: 25226; Mature: 25095

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPIAEPGGHVLVVGASSGIGRAVAERLRAGGARITALARRADRLAELEALGCAAVAAD
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTDLPAIGGVLQAAVAERGLLTGLVYCAGVQVIKPMRSLGVEEVERLYRTNLVAPTLFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
AFGSARVSTRDAVFCAVSSVAAQRPEPAIVPYAASKAGLDALIKGLARELAPRRAVGVAP
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHCCHHHCCCCC
GWLDTEMTQAHARLYGAEFKEALEKRSPAGAATVESVVDAIAFLMSPQARHVTGEILRVD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCEEEEEC
GGAVL
CCCCC
>Mature Secondary Structure 
SQPIAEPGGHVLVVGASSGIGRAVAERLRAGGARITALARRADRLAELEALGCAAVAAD
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTDLPAIGGVLQAAVAERGLLTGLVYCAGVQVIKPMRSLGVEEVERLYRTNLVAPTLFAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
AFGSARVSTRDAVFCAVSSVAAQRPEPAIVPYAASKAGLDALIKGLARELAPRRAVGVAP
HHCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHHHHHHHCCHHHCCCCC
GWLDTEMTQAHARLYGAEFKEALEKRSPAGAATVESVVDAIAFLMSPQARHVTGEILRVD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHCCCEEEEEC
GGAVL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7751271 [H]