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The map label for this gene is glyS

Identifier: 170747570

GI number: 170747570

Start: 1198723

End: 1200822

Strand: Reverse

Name: glyS

Synonym: Mrad2831_1138

Alternate gene names: 170747570

Gene position: 1200822-1198723 (Counterclockwise)

Preceding gene: 170747571

Following gene: 170747567

Centisome position: 19.76

GC content: 73.05

Gene sequence:

>2100_bases
ATGCCCGACCTCCTGCTCGAACTCCGCTCGGAAGAGATCCCCGCCCGCATGCAGCGGCGCGCGGCGGAGGATCTCAAGAA
GCTCGTCACCGACGCCCTCGTGGCGCGCGGCTTCCTGTACGAGGGCGCCCGGGCGTTCTCGACGCCGCGCCGCTTGGCGC
TGCACATCGCCGGCCTGCCCGCCCGGGGCGAGGCCGTGCGCGAGGAGCGCCGCGGCCCGCGGGTCGGCGCCCCCGAGGCG
GCCGTCCAGGGCTTCCTGAAGGGCGCGGGGCTCGCGAGCCTCGATCAGGCCACGACCGTCACCGACCCGAAGAAGGGCGA
GTTCTACCTCGCGGTGATCGAGCGGCCCGGCCGCGAGACGCTCGACGTCCTGGCCGAGTTCCTGCCCGAGATCGTCAGAA
GCTTTCCCTGGCCGAAATCCATGCGCTGGGGCGCGGCCTCGGCCGAGCCGGGATCCTTGCGCTGGGTGCGCCCGCTGCAG
TCGATCGTCGCCACCTTCGGGCCGGAGACCGAGACGCCGGAGGTGATCCCCGTCTCGGTCGGCGGGATCGCGGCCGGCAC
GGTGACCTACGGCCACCGCTTCCTCGCCCCCGAGGCGATCGAGGTGCGCCGGTTCGACGACTACATCCAGGCGCTGGAGC
GCGCGAAGGTCGTCCTCGACGCCGACCGGCGCAAGGACGTGATCCTGCACGACGCCCGCGACCTCGCCTTCGCCCGCGGC
CTCGACCTCGTGGAGGACGAGGGCCTGCTGGAGGAGGTGTCGGGCCTCGTGGAGTGGCCGGTGGTGCTGATGGGCTCGTT
CGACGAGTCCTTCCTCGACATCCCCGCCGAGGCGATCCGCGCGACCATCCGGGCGAACCAGAAGTGCTTCGTGCTGCGCA
AGGGCGGCTCCGAGGACCTGGCGCCGGCCTTCATCCTGGTCTCGAACCTCGTGGCCACCGACGGCGGCCAAGCGATCACC
GCAGGCAACGAGCGGGTGGTCCGGGCGCGGCTCTCGGACGCGAAGTTCTTCTGGGAGACCGACAAGGCGACCCGCCTGGA
AGACCGGCTGCCCAAGCTCGACGCCATCGTCTTCCACGAGAAGCTCGGGACGCAGGGCGAGCGCATCGCCCGCATCGCCG
CCCTGGCGAAGGACCTCGCGCCGCGCGTGGGCGCCGACCCGGCGCTCGCGGAGCGCGCCGCCCGCCTCGCCAAAGCCGAC
CTCGTCACCGAGATGGTCGGCGAGTTCCCCGAGCTCCAGGGCCTGATGGGCCGGAAATACGCGGCGCTCCAGGGCGAGCA
CGACAGCGTCTGCGCCGCCATCGAGGAGCACTACAAGCCGCTCGGCCCCTCCGACCGGGTGCCGACCGATCCGGTCTCCA
TCGCGGTGGCGCTCGCCGACAAGCTCGACACGCTGGCGGGCTTCTGGGCGATCGACGAGAAGCCCACGGGCAGCAAGGAC
CCGTTCGCCCTGCGCCGGGCGGCGCTGGGCGTGATCCGGCTGATCCTCGACCGCGGCCTGCGCCTGCGCCTGCTGGAGCA
GGTCGGCGCGGCGGATCGCAGCCTCGCCGGGCGGGCGGGCGCCGACGCGCGGGACCTCCTCGGCTTCTTCGCGGACCGCC
TGAAGGTCTATCTCCGCGACCAGGGCGCCCGCCACGACCTGATCGACGCCGTCTTCGCCCTGCCCGGCCAGGACGACCTG
CTCATGGTCGTGCGCCGCGTCGAGGCGCTCGCCGCCTTCCTCGACACCGAGGACGGGAAGAACCTGCTCGCGGGCTACAA
GCGCGCGGCCAACATCCTGCGGATCGAGGAGAAGAAGGACGGCCGCGCCTACGATGCCGCGCCCGACGCCGCCCTCGCCG
CCGCGGGCGAGCCGGCCGAGCGGGCGCTGGCCGAGGCCCTGGCCAAGGCGCGGGAGACCGCCTCGGCGGCCGTCGCCCGC
GAGGATTTCTCAGGCGCCATGCAGGCGCTCTCGACGCTGCGCGCGCCGGTGGACGCGTTCTTCCAGGACGTGACCGTCAA
CGCGCCCGACCCGAAGCTCCGCGAGAACCGCTTGGCTCTGCTCAACGCGCTGCGCGCCGCGACGCGGGAAGTGGCGGAGT
TCGCGAAGATCGAGGGGTGA

Upstream 100 bases:

>100_bases
GACCGCAAGTGGCAGCTCTCGACCATCCTCAAGGGCGAGTCGCGCTGAGCCCCGCGCGCCGACCCTCGACAGGGGTGCGG
CGCGGCGATATGGCCGCCGC

Downstream 100 bases:

>100_bases
GCGTCGGCGCACCGAGCGCAGGATCTCGCGCAAGTCTCCCTAGAGGGGACTTGCGCCTCGTACCGACCCTTGAGGCCCCT
CACCCGTGATCGTGCCCGGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVARGFLYEGARAFSTPRRLALHIAGLPARGEAVREERRGPRVGAPEA
AVQGFLKGAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEFLPEIVRSFPWPKSMRWGAASAEPGSLRWVRPLQ
SIVATFGPETETPEVIPVSVGGIAAGTVTYGHRFLAPEAIEVRRFDDYIQALERAKVVLDADRRKDVILHDARDLAFARG
LDLVEDEGLLEEVSGLVEWPVVLMGSFDESFLDIPAEAIRATIRANQKCFVLRKGGSEDLAPAFILVSNLVATDGGQAIT
AGNERVVRARLSDAKFFWETDKATRLEDRLPKLDAIVFHEKLGTQGERIARIAALAKDLAPRVGADPALAERAARLAKAD
LVTEMVGEFPELQGLMGRKYAALQGEHDSVCAAIEEHYKPLGPSDRVPTDPVSIAVALADKLDTLAGFWAIDEKPTGSKD
PFALRRAALGVIRLILDRGLRLRLLEQVGAADRSLAGRAGADARDLLGFFADRLKVYLRDQGARHDLIDAVFALPGQDDL
LMVVRRVEALAAFLDTEDGKNLLAGYKRAANILRIEEKKDGRAYDAAPDAALAAAGEPAERALAEALAKARETASAAVAR
EDFSGAMQALSTLRAPVDAFFQDVTVNAPDPKLRENRLALLNALRAATREVAEFAKIEG

Sequences:

>Translated_699_residues
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVARGFLYEGARAFSTPRRLALHIAGLPARGEAVREERRGPRVGAPEA
AVQGFLKGAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEFLPEIVRSFPWPKSMRWGAASAEPGSLRWVRPLQ
SIVATFGPETETPEVIPVSVGGIAAGTVTYGHRFLAPEAIEVRRFDDYIQALERAKVVLDADRRKDVILHDARDLAFARG
LDLVEDEGLLEEVSGLVEWPVVLMGSFDESFLDIPAEAIRATIRANQKCFVLRKGGSEDLAPAFILVSNLVATDGGQAIT
AGNERVVRARLSDAKFFWETDKATRLEDRLPKLDAIVFHEKLGTQGERIARIAALAKDLAPRVGADPALAERAARLAKAD
LVTEMVGEFPELQGLMGRKYAALQGEHDSVCAAIEEHYKPLGPSDRVPTDPVSIAVALADKLDTLAGFWAIDEKPTGSKD
PFALRRAALGVIRLILDRGLRLRLLEQVGAADRSLAGRAGADARDLLGFFADRLKVYLRDQGARHDLIDAVFALPGQDDL
LMVVRRVEALAAFLDTEDGKNLLAGYKRAANILRIEEKKDGRAYDAAPDAALAAAGEPAERALAEALAKARETASAAVAR
EDFSGAMQALSTLRAPVDAFFQDVTVNAPDPKLRENRLALLNALRAATREVAEFAKIEG
>Mature_698_residues
PDLLLELRSEEIPARMQRRAAEDLKKLVTDALVARGFLYEGARAFSTPRRLALHIAGLPARGEAVREERRGPRVGAPEAA
VQGFLKGAGLASLDQATTVTDPKKGEFYLAVIERPGRETLDVLAEFLPEIVRSFPWPKSMRWGAASAEPGSLRWVRPLQS
IVATFGPETETPEVIPVSVGGIAAGTVTYGHRFLAPEAIEVRRFDDYIQALERAKVVLDADRRKDVILHDARDLAFARGL
DLVEDEGLLEEVSGLVEWPVVLMGSFDESFLDIPAEAIRATIRANQKCFVLRKGGSEDLAPAFILVSNLVATDGGQAITA
GNERVVRARLSDAKFFWETDKATRLEDRLPKLDAIVFHEKLGTQGERIARIAALAKDLAPRVGADPALAERAARLAKADL
VTEMVGEFPELQGLMGRKYAALQGEHDSVCAAIEEHYKPLGPSDRVPTDPVSIAVALADKLDTLAGFWAIDEKPTGSKDP
FALRRAALGVIRLILDRGLRLRLLEQVGAADRSLAGRAGADARDLLGFFADRLKVYLRDQGARHDLIDAVFALPGQDDLL
MVVRRVEALAAFLDTEDGKNLLAGYKRAANILRIEEKKDGRAYDAAPDAALAAAGEPAERALAEALAKARETASAAVARE
DFSGAMQALSTLRAPVDAFFQDVTVNAPDPKLRENRLALLNALRAATREVAEFAKIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=711, Percent_Identity=37.9746835443038, Blast_Score=391, Evalue=1e-110,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_METRJ (B1M1W2)

Other databases:

- EMBL:   CP001001
- RefSeq:   YP_001753830.1
- ProteinModelPortal:   B1M1W2
- GeneID:   6137155
- GenomeReviews:   CP001001_GR
- KEGG:   mrd:Mrad2831_1138
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 75916; Mature: 75784

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELRSEEIPARMQRRAAEDLKKLVTDALVARGFLYEGARAFSTPRRLALHIAGLP
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHEEEEEECCC
ARGEAVREERRGPRVGAPEAAVQGFLKGAGLASLDQATTVTDPKKGEFYLAVIERPGRET
CCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LDVLAEFLPEIVRSFPWPKSMRWGAASAEPGSLRWVRPLQSIVATFGPETETPEVIPVSV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
GGIAAGTVTYGHRFLAPEAIEVRRFDDYIQALERAKVVLDADRRKDVILHDARDLAFARG
CCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHC
LDLVEDEGLLEEVSGLVEWPVVLMGSFDESFLDIPAEAIRATIRANQKCFVLRKGGSEDL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
APAFILVSNLVATDGGQAITAGNERVVRARLSDAKFFWETDKATRLEDRLPKLDAIVFHE
HHHHHHHHHHHHCCCCCEEECCCCCEEHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHH
KLGTQGERIARIAALAKDLAPRVGADPALAERAARLAKADLVTEMVGEFPELQGLMGRKY
HHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCH
AALQGEHDSVCAAIEEHYKPLGPSDRVPTDPVSIAVALADKLDTLAGFWAIDEKPTGSKD
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PFALRRAALGVIRLILDRGLRLRLLEQVGAADRSLAGRAGADARDLLGFFADRLKVYLRD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
QGARHDLIDAVFALPGQDDLLMVVRRVEALAAFLDTEDGKNLLAGYKRAANILRIEEKKD
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCC
GRAYDAAPDAALAAAGEPAERALAEALAKARETASAAVAREDFSGAMQALSTLRAPVDAF
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQDVTVNAPDPKLRENRLALLNALRAATREVAEFAKIEG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDLLLELRSEEIPARMQRRAAEDLKKLVTDALVARGFLYEGARAFSTPRRLALHIAGLP
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCHHEEEEEECCC
ARGEAVREERRGPRVGAPEAAVQGFLKGAGLASLDQATTVTDPKKGEFYLAVIERPGRET
CCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHH
LDVLAEFLPEIVRSFPWPKSMRWGAASAEPGSLRWVRPLQSIVATFGPETETPEVIPVSV
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
GGIAAGTVTYGHRFLAPEAIEVRRFDDYIQALERAKVVLDADRRKDVILHDARDLAFARG
CCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHC
LDLVEDEGLLEEVSGLVEWPVVLMGSFDESFLDIPAEAIRATIRANQKCFVLRKGGSEDL
CCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHCCCEEEEEECCCCCCH
APAFILVSNLVATDGGQAITAGNERVVRARLSDAKFFWETDKATRLEDRLPKLDAIVFHE
HHHHHHHHHHHHCCCCCEEECCCCCEEHHHHCCCCEEEECCCHHHHHHHCCHHHHHHHHH
KLGTQGERIARIAALAKDLAPRVGADPALAERAARLAKADLVTEMVGEFPELQGLMGRKY
HHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCH
AALQGEHDSVCAAIEEHYKPLGPSDRVPTDPVSIAVALADKLDTLAGFWAIDEKPTGSKD
HHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCC
PFALRRAALGVIRLILDRGLRLRLLEQVGAADRSLAGRAGADARDLLGFFADRLKVYLRD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
QGARHDLIDAVFALPGQDDLLMVVRRVEALAAFLDTEDGKNLLAGYKRAANILRIEEKKD
CCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEECCCC
GRAYDAAPDAALAAAGEPAERALAEALAKARETASAAVAREDFSGAMQALSTLRAPVDAF
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FQDVTVNAPDPKLRENRLALLNALRAATREVAEFAKIEG
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA