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The map label for this gene is ygbJ [C]

Identifier: 170747566

GI number: 170747566

Start: 1194314

End: 1195222

Strand: Reverse

Name: ygbJ [C]

Synonym: Mrad2831_1134

Alternate gene names: 170747566

Gene position: 1195222-1194314 (Counterclockwise)

Preceding gene: 170747567

Following gene: 170747565

Centisome position: 19.67

GC content: 73.82

Gene sequence:

>909_bases
ATGACCAACGCAACACACCGCGCCGCGGTGATCGGCCTGGGCTCCATGGGCTCCGGCATGGCCGGCTCGCTCCTGCGCGC
GGGCTTCTCGGTCGCCGCCTGCGACGTGAACCCGGAGGCGGTGGCGCGCTTCGCGGCCGCCGGCGGGCGCGCCGCCGCGA
ACCCCGCCGAGGCGGCGCGCGACGCCGACGTGGTGGTGAGCGTGGTGGTCAACGCGGCCCAGACCGAGGCGGTGCTGTTC
GGCGAGAACGGCGCCGCCGCCGCGATGCCGGAGGGCGCGGTGTTCGTCTCCTCCGCCACCATGGATCCGGCCGTCGCCCG
GGCGCTGGCGGCGCGGCTGGAGGCCACCGGCCGCCACTATCTCGACGCCCCGATGAGCGGCGGCGCCGCCCGCGCGGCAG
AGGGCGGCCTGACCTTCCTGGCCTCGGGCTCGACGGCGGCCTTCGCCAAGGCGCGACCCGCCCTCGATGCCATGGCCGGC
ACGCTCTACGAGCTCGGCGACGCGGCCGGCCAGGGCGCGGCCTTCAAGATGATCAACCAGCTGCTCGCGGGCGTGCACAT
CGCCGCCGCCGCCGAGTCCATGGCCTTCGCGGCCAAGCAGGGGCTCGACCTCCGGCGCGTCTACGAGGTGATCACCAAGT
CGGCCGGCAATTCCTGGATGTTCGAGAACCGGGTGCCGCACATCCTCGACGCCGACTACAGCCCGAAGAGCGCGGTCGAC
ATCTTCGTCAAGGATCTCGGCATCGTGCAGGACATGGCCCGGGCCGAGAAGTACCCGGTCCCGGTGGCGGCGGCGGCCCT
GCAGATGTTCCTGATGGCGTCCGGCGCCGGTATGGGCCGGGACGACGACGCCTCGGTGGCGCGGCTCTACGCGCAGGTCT
CGGGCGCGAAGCTGCCGGGCCAGAAGTAG

Upstream 100 bases:

>100_bases
GGATCCCGGCACGACCCGCCCCTCTCCCGCACGGGAGAGGGGACTTGCGTCTCACGCGGTGATGCGGAGGCGCCCTCCCC
TTCATGCAGGAGCCGCCCCG

Downstream 100 bases:

>100_bases
CCGGATCGAGCGCAGGGAGTTCGAAGACAATGCCGCGCTTTGCCGCAAACCTGAGCCTCATGTTCAACGAGGTGCCCTTC
CTCGACCGGTTCGAGGCCGC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTNATHRAAVIGLGSMGSGMAGSLLRAGFSVAACDVNPEAVARFAAAGGRAAANPAEAARDADVVVSVVVNAAQTEAVLF
GENGAAAAMPEGAVFVSSATMDPAVARALAARLEATGRHYLDAPMSGGAARAAEGGLTFLASGSTAAFAKARPALDAMAG
TLYELGDAAGQGAAFKMINQLLAGVHIAAAAESMAFAAKQGLDLRRVYEVITKSAGNSWMFENRVPHILDADYSPKSAVD
IFVKDLGIVQDMARAEKYPVPVAAAALQMFLMASGAGMGRDDDASVARLYAQVSGAKLPGQK

Sequences:

>Translated_302_residues
MTNATHRAAVIGLGSMGSGMAGSLLRAGFSVAACDVNPEAVARFAAAGGRAAANPAEAARDADVVVSVVVNAAQTEAVLF
GENGAAAAMPEGAVFVSSATMDPAVARALAARLEATGRHYLDAPMSGGAARAAEGGLTFLASGSTAAFAKARPALDAMAG
TLYELGDAAGQGAAFKMINQLLAGVHIAAAAESMAFAAKQGLDLRRVYEVITKSAGNSWMFENRVPHILDADYSPKSAVD
IFVKDLGIVQDMARAEKYPVPVAAAALQMFLMASGAGMGRDDDASVARLYAQVSGAKLPGQK
>Mature_301_residues
TNATHRAAVIGLGSMGSGMAGSLLRAGFSVAACDVNPEAVARFAAAGGRAAANPAEAARDADVVVSVVVNAAQTEAVLFG
ENGAAAAMPEGAVFVSSATMDPAVARALAARLEATGRHYLDAPMSGGAARAAEGGLTFLASGSTAAFAKARPALDAMAGT
LYELGDAAGQGAAFKMINQLLAGVHIAAAAESMAFAAKQGLDLRRVYEVITKSAGNSWMFENRVPHILDADYSPKSAVDI
FVKDLGIVQDMARAEKYPVPVAAAALQMFLMASGAGMGRDDDASVARLYAQVSGAKLPGQK

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=286, Percent_Identity=31.8181818181818, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI40556376, Length=290, Percent_Identity=25.1724137931034, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1789092, Length=302, Percent_Identity=48.0132450331126, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI145693186, Length=288, Percent_Identity=31.25, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1790315, Length=279, Percent_Identity=34.0501792114695, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=27.0547945205479, Blast_Score=122, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557316, Length=268, Percent_Identity=30.9701492537313, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24655230, Length=289, Percent_Identity=28.719723183391, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922568, Length=289, Percent_Identity=28.719723183391, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI28574115, Length=277, Percent_Identity=23.826714801444, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.-.- [C]

Molecular weight: Translated: 30549; Mature: 30418

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNATHRAAVIGLGSMGSGMAGSLLRAGFSVAACDVNPEAVARFAAAGGRAAANPAEAAR
CCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHH
DADVVVSVVVNAAQTEAVLFGENGAAAAMPEGAVFVSSATMDPAVARALAARLEATGRHY
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEE
LDAPMSGGAARAAEGGLTFLASGSTAAFAKARPALDAMAGTLYELGDAAGQGAAFKMINQ
ECCCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAGVHIAAAAESMAFAAKQGLDLRRVYEVITKSAGNSWMFENRVPHILDADYSPKSAVD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCEECCCCCCHHHHH
IFVKDLGIVQDMARAEKYPVPVAAAALQMFLMASGAGMGRDDDASVARLYAQVSGAKLPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
QK
CC
>Mature Secondary Structure 
TNATHRAAVIGLGSMGSGMAGSLLRAGFSVAACDVNPEAVARFAAAGGRAAANPAEAAR
CCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHH
DADVVVSVVVNAAQTEAVLFGENGAAAAMPEGAVFVSSATMDPAVARALAARLEATGRHY
HHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEE
LDAPMSGGAARAAEGGLTFLASGSTAAFAKARPALDAMAGTLYELGDAAGQGAAFKMINQ
ECCCCCCCCCHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LLAGVHIAAAAESMAFAAKQGLDLRRVYEVITKSAGNSWMFENRVPHILDADYSPKSAVD
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCCCCEECCCCCCHHHHH
IFVKDLGIVQDMARAEKYPVPVAAAALQMFLMASGAGMGRDDDASVARLYAQVSGAKLPG
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]