Click here to switch to the map view.

The map label for this gene is fdoI [H]

Identifier: 170747522

GI number: 170747522

Start: 1146701

End: 1147423

Strand: Reverse

Name: fdoI [H]

Synonym: Mrad2831_1090

Alternate gene names: 170747522

Gene position: 1147423-1146701 (Counterclockwise)

Preceding gene: 170747523

Following gene: 170747521

Centisome position: 18.88

GC content: 67.36

Gene sequence:

>723_bases
ATGACGATCCACAGCGACATCCGGGACGGCGATCCCCGCCCCCTCCACCACGCCAAGTCCGCGGCACCGGAGGTGATGTA
CGTCCCGCGCTACACCGGCGTGCAGCGGGTGAACCACTGGATCACCGCGATCCTGTTCACCTTGCTGACCCTGTCGGGTC
TGGCGATGTTCACGCCCTACCTGTTCTCGCTGACCGGCCTGTTCGGCGGCGGGCAGGCGACCCGGGCGATCCACCCGTGG
TTCGGCGTGGCGCTGGCGGTCAGCTTCTTCTTCCTGTTCGTGCGCTTCTGGAAGCTCAACATCCCCAACAAGGACGATGT
CGAGTGGACCAAGCACATCGGCGACGTGGTCACCAACCGCGACGACCGGCTGCCGGAGCTCGGCAAGTACAATGCCGGCC
AGAAGGGCGTGTTCTGGGGCCAGACCGCGCTGATCGGCGTGATGTTCGTCACCGGCCTCGTGATCTGGAACACCTATTTC
GGCGGCCTCACCTCGATCGAGACCCAGCGCTGGGCGCTGCTCGCCCATTCGCTCGCCGCCGTGATCGCCATCGCGATCAT
CGTGGTGCACATCTACGCCGGCATCTGGGTGCGCGGCACCGGCCGGGCGATGGTCCGCGGGACGGTCACGGGCGGCTGGG
CCTACCGGCATCACCGCAAGTGGTTCCGGCAGATCGCGGGCGGCAAGGCGCGCCACGGCTCGGTGGACAAGCGCGGATCC
TGA

Upstream 100 bases:

>100_bases
GACAGGGTGGAGACGGTGCAGCCGCCCCACCCTATCTCGAAGCTCAGAGCCACCTCCGCCGTGATGCGGGGCCGGCACCG
CATCAGGCGCGAGAAGGCCG

Downstream 100 bases:

>100_bases
GTCTTGGCCAACTTCTTCAAGCGTAAACCCGCGGTTCCGGCCGCGGACAAGACCCCGACACCCGCCGAGGCCCCGAAGCC
CCGCCGCCGGGATTTCAGCG

Product: formate dehydrogenase subunit gamma

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTIHSDIRDGDPRPLHHAKSAAPEVMYVPRYTGVQRVNHWITAILFTLLTLSGLAMFTPYLFSLTGLFGGGQATRAIHPW
FGVALAVSFFFLFVRFWKLNIPNKDDVEWTKHIGDVVTNRDDRLPELGKYNAGQKGVFWGQTALIGVMFVTGLVIWNTYF
GGLTSIETQRWALLAHSLAAVIAIAIIVVHIYAGIWVRGTGRAMVRGTVTGGWAYRHHRKWFRQIAGGKARHGSVDKRGS

Sequences:

>Translated_240_residues
MTIHSDIRDGDPRPLHHAKSAAPEVMYVPRYTGVQRVNHWITAILFTLLTLSGLAMFTPYLFSLTGLFGGGQATRAIHPW
FGVALAVSFFFLFVRFWKLNIPNKDDVEWTKHIGDVVTNRDDRLPELGKYNAGQKGVFWGQTALIGVMFVTGLVIWNTYF
GGLTSIETQRWALLAHSLAAVIAIAIIVVHIYAGIWVRGTGRAMVRGTVTGGWAYRHHRKWFRQIAGGKARHGSVDKRGS
>Mature_239_residues
TIHSDIRDGDPRPLHHAKSAAPEVMYVPRYTGVQRVNHWITAILFTLLTLSGLAMFTPYLFSLTGLFGGGQATRAIHPWF
GVALAVSFFFLFVRFWKLNIPNKDDVEWTKHIGDVVTNRDDRLPELGKYNAGQKGVFWGQTALIGVMFVTGLVIWNTYFG
GLTSIETQRWALLAHSLAAVIAIAIIVVHIYAGIWVRGTGRAMVRGTVTGGWAYRHHRKWFRQIAGGKARHGSVDKRGS

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=198, Percent_Identity=43.4343434343434, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI1787750, Length=209, Percent_Identity=39.2344497607655, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 26815; Mature: 26684

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure 
TIHSDIRDGDPRPLHHAKSAAPEVMYVPRYTGVQRVNHWITAILFTLLTLSGLAMFTPY
CCCCCCCCCCCCCCHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFSLTGLFGGGQATRAIHPWFGVALAVSFFFLFVRFWKLNIPNKDDVEWTKHIGDVVTNR
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
DDRLPELGKYNAGQKGVFWGQTALIGVMFVTGLVIWNTYFGGLTSIETQRWALLAHSLAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VIAIAIIVVHIYAGIWVRGTGRAMVRGTVTGGWAYRHHRKWFRQIAGGKARHGSVDKRGS
HHHHHHHHHHHHHHHEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]