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The map label for this gene is fdoH [H]

Identifier: 170747523

GI number: 170747523

Start: 1147724

End: 1148659

Strand: Reverse

Name: fdoH [H]

Synonym: Mrad2831_1091

Alternate gene names: 170747523

Gene position: 1148659-1147724 (Counterclockwise)

Preceding gene: 170747524

Following gene: 170747522

Centisome position: 18.9

GC content: 67.09

Gene sequence:

>936_bases
ATGGCTGACTACAGCTCCCTCGACATCCGCCAGCGCTCCGCCTCCACGGAGACGCCGCCGGAGATCCGCCGCCAGGTGGA
GGTCGCCAAGCTCATCGACGTGTCGAAGTGCATCGGCTGCAAGGCCTGCCAGTCGGCCTGCGAGGAGTGGAACGACCTGC
GCGACGACATCGGTGTCAACACGGGCACCTACCAGAACCCTCACGACCTCACGCCGAAGTCGTGGACGCTGATGCGGTTC
ACCGAGTACGAGAACCCGGAGACCAACAACCTCGAATGGCTGATCCGCAAGGACGGCTGCATGCACTGCACCGAGCCGGG
CTGCCTGAAGGCCTGCCCGTCGCCCGGCGCGATCGTGCAGTACTCCAACGGCATCGTCGACTTCATCGAGGAGAACTGCA
TCGGCTGCGGCTACTGCGTGAAGGGCTGCCCCTTCAACATCCCGCGCATCAGCCAGACCGACCACAAGGCGTACAAGTGC
ACCCTGTGCTCGGACCGGGTGGCGGTGGGTCAGGCCCCGGCCTGCGCCAAGGCCTGCCCGACCGGCTCGATCATGTTCGG
CACCAAGCAGGCCATGATCGATCAGGCCCACGACCGCGTCGAGGACCTGAAGTCGCGCGGGTTCGACAAGGCGGGTCTCT
ACGACCCGGCCGGGGTCGGCGGCACGCACGTCATGTACGTGCTGCACCACGCCGACCAGCCGAGCCTCTATGCCGGTCTG
CCGAACGACCCGAAGATCTCGCCGCTGGTCGCCTTCTGGAAGGGCGGCGCCAAGGTGTTCGGTCTCGCCGCGATGGGCTT
CGCGGCAGTGGCGGGCTTCTTCCACTACGTCACGGCCGGCCCGAACGAGGTCGTGCCCGAGGAGGAGGAAGAGGCGGTCG
AGTACGACGAGGCCAAGCGCCGGGAGAATGGCGGCGGCGAGATCCGGCCGCACTGA

Upstream 100 bases:

>100_bases
CGACGCGAACACCGAGACGCCGGAGTTCAAGGCCTGGCTCGTGAACATCGAGCCGACCACGCCTCCGTCGGACGCGGTCG
CGTAAGGGGGAGCGGCAGAC

Downstream 100 bases:

>100_bases
CCCAACGGTCTCCCTCCCCCTCTGCAGGGGAGAGAGACCCGCGAAGCGGATAGGACAGGGGAGCGACGGTGCAGAACAGA
CCTCTGCCCGTCGTGCTGGT

Product: formate dehydrogenase subunit beta

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase iron-sulfur subunit; FDH-Z subunit beta; Formate dehydrogenase-O subunit beta [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MADYSSLDIRQRSASTETPPEIRRQVEVAKLIDVSKCIGCKACQSACEEWNDLRDDIGVNTGTYQNPHDLTPKSWTLMRF
TEYENPETNNLEWLIRKDGCMHCTEPGCLKACPSPGAIVQYSNGIVDFIEENCIGCGYCVKGCPFNIPRISQTDHKAYKC
TLCSDRVAVGQAPACAKACPTGSIMFGTKQAMIDQAHDRVEDLKSRGFDKAGLYDPAGVGGTHVMYVLHHADQPSLYAGL
PNDPKISPLVAFWKGGAKVFGLAAMGFAAVAGFFHYVTAGPNEVVPEEEEEAVEYDEAKRRENGGGEIRPH

Sequences:

>Translated_311_residues
MADYSSLDIRQRSASTETPPEIRRQVEVAKLIDVSKCIGCKACQSACEEWNDLRDDIGVNTGTYQNPHDLTPKSWTLMRF
TEYENPETNNLEWLIRKDGCMHCTEPGCLKACPSPGAIVQYSNGIVDFIEENCIGCGYCVKGCPFNIPRISQTDHKAYKC
TLCSDRVAVGQAPACAKACPTGSIMFGTKQAMIDQAHDRVEDLKSRGFDKAGLYDPAGVGGTHVMYVLHHADQPSLYAGL
PNDPKISPLVAFWKGGAKVFGLAAMGFAAVAGFFHYVTAGPNEVVPEEEEEAVEYDEAKRRENGGGEIRPH
>Mature_310_residues
ADYSSLDIRQRSASTETPPEIRRQVEVAKLIDVSKCIGCKACQSACEEWNDLRDDIGVNTGTYQNPHDLTPKSWTLMRFT
EYENPETNNLEWLIRKDGCMHCTEPGCLKACPSPGAIVQYSNGIVDFIEENCIGCGYCVKGCPFNIPRISQTDHKAYKCT
LCSDRVAVGQAPACAKACPTGSIMFGTKQAMIDQAHDRVEDLKSRGFDKAGLYDPAGVGGTHVMYVLHHADQPSLYAGLP
NDPKISPLVAFWKGGAKVFGLAAMGFAAVAGFFHYVTAGPNEVVPEEEEEAVEYDEAKRRENGGGEIRPH

Specific function: Allows to use formate as major electron donor during aerobic respiration. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iron- sulfur centers. Electrons are transferred from the gamma chain to the m

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=302, Percent_Identity=62.9139072847682, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI1787749, Length=289, Percent_Identity=64.3598615916955, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789370, Length=184, Percent_Identity=29.8913043478261, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787122, Length=175, Percent_Identity=32.5714285714286, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787872, Length=157, Percent_Identity=32.484076433121, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI2367345, Length=156, Percent_Identity=30.1282051282051, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=174, Percent_Identity=28.735632183908, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787963, Length=150, Percent_Identity=26, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788811, Length=176, Percent_Identity=26.7045454545455, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787478, Length=229, Percent_Identity=24.4541484716157, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787740, Length=106, Percent_Identity=30.188679245283, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: 1.2.1.2

Molecular weight: Translated: 34033; Mature: 33901

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
5.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADYSSLDIRQRSASTETPPEIRRQVEVAKLIDVSKCIGCKACQSACEEWNDLRDDIGVN
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
TGTYQNPHDLTPKSWTLMRFTEYENPETNNLEWLIRKDGCMHCTEPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEE
YSNGIVDFIEENCIGCGYCVKGCPFNIPRISQTDHKAYKCTLCSDRVAVGQAPACAKACP
ECCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCHHHHHCC
TGSIMFGTKQAMIDQAHDRVEDLKSRGFDKAGLYDPAGVGGTHVMYVLHHADQPSLYAGL
CCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECC
PNDPKISPLVAFWKGGAKVFGLAAMGFAAVAGFFHYVTAGPNEVVPEEEEEAVEYDEAKR
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RENGGGEIRPH
HCCCCCCCCCC
>Mature Secondary Structure 
ADYSSLDIRQRSASTETPPEIRRQVEVAKLIDVSKCIGCKACQSACEEWNDLRDDIGVN
CCCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCC
TGTYQNPHDLTPKSWTLMRFTEYENPETNNLEWLIRKDGCMHCTEPGCLKACPSPGAIVQ
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCHHHCCCCCCCEEE
YSNGIVDFIEENCIGCGYCVKGCPFNIPRISQTDHKAYKCTLCSDRVAVGQAPACAKACP
ECCCHHHHHHCCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHCCCCCHHHHHCC
TGSIMFGTKQAMIDQAHDRVEDLKSRGFDKAGLYDPAGVGGTHVMYVLHHADQPSLYAGL
CCCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCEEECC
PNDPKISPLVAFWKGGAKVFGLAAMGFAAVAGFFHYVTAGPNEVVPEEEEEAVEYDEAKR
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
RENGGGEIRPH
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]