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The map label for this gene is purK [H]

Identifier: 170747495

GI number: 170747495

Start: 1121830

End: 1122942

Strand: Reverse

Name: purK [H]

Synonym: Mrad2831_1063

Alternate gene names: 170747495

Gene position: 1122942-1121830 (Counterclockwise)

Preceding gene: 170747496

Following gene: 170747494

Centisome position: 18.48

GC content: 73.76

Gene sequence:

>1113_bases
GTGACGGACAAGATCCTCGGCCCCGGCTTCACCCTCGGCATCGTCGGCGGGGGTCAGCTCGGGCGCATGATCGCGCTCGC
GGCGGCCGATTACGGCCTCAAGGCGCATGTCTACGCGCCCGACGCGGACAGCCCCGCCTTCGACGTGGCCGCCCGGACCA
CCTGCGCGGCCTACGACGACGCCGCGGCGCTGGCGGATTTCGCCCGCAGCGTCGACGTCGTGACCTACGAGTTCGAGAAC
ATCCCGCACGCCACCGCCGACGTGCTGGCCCGGCACGCCGCCCTGCACCCGAACGCGCGGGCGCTCTCGACCACGCAGGA
CCGCCTCTCCGAGAAGGAGTTCATCAACGGCCTAGGCATCCCGACGGCGCCGTTCCGGGCGGTGGACACCCCCGACGACC
TCGACCGGGCGATCGAGGTGATCGGCCTGCCGGCCGTGCTCAAGACCCGCCGCTTCGGCTACGACGGCAAGGGGCAGCGC
ATGCTCCGCGAGCGGGCCGAGGGCGACCGCAACGCCCTGTTCGCCGAGTTCGGCGGCGCGCCGCTGATCCTGGAGGGCTT
CGTGCCGTTCGAGCGCGAGGTCTCGGTGGTCGGCGCGCGCGGGCGCGACGGCAGCTTCGCGGCCTACGACCTCTGCGAGA
ACGAACACCGCGACCACATCCTGGCGCTGACCCGGGTGCCGGCGCCCGGCGTGTCCGCCGAGACGGGCCGCGCCGCCGTC
GAGATCGCCCGGCGGATCGCCGACGCCCTCGACTACGTCGGCGTGCTGGCCGTCGAGATGTTCCTCGTCCGCGAGGACGG
GGCTGAGCGGCTCGTGGTCAACGAGATCGCGCCCCGGGTCCACAATTCCGGCCACTGGACCAGCGAGGGGGCGCTGACCT
CGCAGTTCGCCCAGCACGTCCGGGCCGTCTGCGGCTGGCCGCTGGGCGATCCGGGCCGGGTCGGCGGCCTCCCCGTCGAG
ATGCGCAACCTGATCGGCGCCCAGGCCGACGACTGGCACGCCCTGCTGGCCGAGCCCGGCGCCCACCTGCACCTCTACGG
CAAGGGCGAAGCGCGCCCCGGCCGCAAGATGGGTCACGTCACCCGCCTCGGGGCCGGCCCGACCGGTTCCTGA

Upstream 100 bases:

>100_bases
TCCTGGCGCTGACCGACCCGGGCCTGGCGGAGCGCCTGGAGGCGTGGCGCGCGGCCCAGAGCGCCTCGGTGGCCGAACGG
CCGGTGACGGAAGAGAACGC

Downstream 100 bases:

>100_bases
GGCTTCGAAGGCACACAAGCGCCGCCGCATGACCGCCGGAGGTCCGTCCGCGGCCCCGCGCGACGGTCGAGCCATACCTT
CGCCACATCCCGCCGCTTCC

Product: phosphoribosylaminoimidazole carboxylase, ATPase

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MTDKILGPGFTLGIVGGGQLGRMIALAAADYGLKAHVYAPDADSPAFDVAARTTCAAYDDAAALADFARSVDVVTYEFEN
IPHATADVLARHAALHPNARALSTTQDRLSEKEFINGLGIPTAPFRAVDTPDDLDRAIEVIGLPAVLKTRRFGYDGKGQR
MLRERAEGDRNALFAEFGGAPLILEGFVPFEREVSVVGARGRDGSFAAYDLCENEHRDHILALTRVPAPGVSAETGRAAV
EIARRIADALDYVGVLAVEMFLVREDGAERLVVNEIAPRVHNSGHWTSEGALTSQFAQHVRAVCGWPLGDPGRVGGLPVE
MRNLIGAQADDWHALLAEPGAHLHLYGKGEARPGRKMGHVTRLGAGPTGS

Sequences:

>Translated_370_residues
MTDKILGPGFTLGIVGGGQLGRMIALAAADYGLKAHVYAPDADSPAFDVAARTTCAAYDDAAALADFARSVDVVTYEFEN
IPHATADVLARHAALHPNARALSTTQDRLSEKEFINGLGIPTAPFRAVDTPDDLDRAIEVIGLPAVLKTRRFGYDGKGQR
MLRERAEGDRNALFAEFGGAPLILEGFVPFEREVSVVGARGRDGSFAAYDLCENEHRDHILALTRVPAPGVSAETGRAAV
EIARRIADALDYVGVLAVEMFLVREDGAERLVVNEIAPRVHNSGHWTSEGALTSQFAQHVRAVCGWPLGDPGRVGGLPVE
MRNLIGAQADDWHALLAEPGAHLHLYGKGEARPGRKMGHVTRLGAGPTGS
>Mature_369_residues
TDKILGPGFTLGIVGGGQLGRMIALAAADYGLKAHVYAPDADSPAFDVAARTTCAAYDDAAALADFARSVDVVTYEFENI
PHATADVLARHAALHPNARALSTTQDRLSEKEFINGLGIPTAPFRAVDTPDDLDRAIEVIGLPAVLKTRRFGYDGKGQRM
LRERAEGDRNALFAEFGGAPLILEGFVPFEREVSVVGARGRDGSFAAYDLCENEHRDHILALTRVPAPGVSAETGRAAVE
IARRIADALDYVGVLAVEMFLVREDGAERLVVNEIAPRVHNSGHWTSEGALTSQFAQHVRAVCGWPLGDPGRVGGLPVEM
RNLIGAQADDWHALLAEPGAHLHLYGKGEARPGRKMGHVTRLGAGPTGS

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=350, Percent_Identity=38.5714285714286, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1788155, Length=358, Percent_Identity=26.2569832402235, Blast_Score=86, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324702, Length=375, Percent_Identity=30.6666666666667, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 39450; Mature: 39319

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKILGPGFTLGIVGGGQLGRMIALAAADYGLKAHVYAPDADSPAFDVAARTTCAAYDD
CCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
AAALADFARSVDVVTYEFENIPHATADVLARHAALHPNARALSTTQDRLSEKEFINGLGI
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCC
PTAPFRAVDTPDDLDRAIEVIGLPAVLKTRRFGYDGKGQRMLRERAEGDRNALFAEFGGA
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCC
PLILEGFVPFEREVSVVGARGRDGSFAAYDLCENEHRDHILALTRVPAPGVSAETGRAAV
CEEEECCCCCCCCEEEEECCCCCCCEEEHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHH
EIARRIADALDYVGVLAVEMFLVREDGAERLVVNEIAPRVHNSGHWTSEGALTSQFAQHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RAVCGWPLGDPGRVGGLPVEMRNLIGAQADDWHALLAEPGAHLHLYGKGEARPGRKMGHV
HHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCCCHHCCE
TRLGAGPTGS
EEECCCCCCC
>Mature Secondary Structure 
TDKILGPGFTLGIVGGGQLGRMIALAAADYGLKAHVYAPDADSPAFDVAARTTCAAYDD
CCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHH
AAALADFARSVDVVTYEFENIPHATADVLARHAALHPNARALSTTQDRLSEKEFINGLGI
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHCCCC
PTAPFRAVDTPDDLDRAIEVIGLPAVLKTRRFGYDGKGQRMLRERAEGDRNALFAEFGGA
CCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHCCCCCCEEEECCCCC
PLILEGFVPFEREVSVVGARGRDGSFAAYDLCENEHRDHILALTRVPAPGVSAETGRAAV
CEEEECCCCCCCCEEEEECCCCCCCEEEHHHHCCCCCCEEEEEEECCCCCCCCCCHHHHH
EIARRIADALDYVGVLAVEMFLVREDGAERLVVNEIAPRVHNSGHWTSEGALTSQFAQHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
RAVCGWPLGDPGRVGGLPVEMRNLIGAQADDWHALLAEPGAHLHLYGKGEARPGRKMGHV
HHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHCCCCCEEEEEECCCCCCCCHHCCE
TRLGAGPTGS
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]