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The map label for this gene is paaF [H]

Identifier: 170747488

GI number: 170747488

Start: 1112638

End: 1113375

Strand: Reverse

Name: paaF [H]

Synonym: Mrad2831_1056

Alternate gene names: 170747488

Gene position: 1113375-1112638 (Counterclockwise)

Preceding gene: 170747489

Following gene: 170747487

Centisome position: 18.32

GC content: 76.56

Gene sequence:

>738_bases
GTGAGCGTCCGCGTCGCCGATCCGGAGCCCGGGATCCGGCTCGTCACGATGGACCGGCCGGAGCGGCGCAACGCCCTCGA
CCGGGCGACCTACGCGGGCCTGACCGACGCCCTGGACACGGCGGGAAGGGACGCGGAGGTGCGCGCCCTCGTCCTCACCG
GCGCGGGCGGCTGCTTCACCGCCGGCAACGATCTCAAGGATTTCCAGGACGTCGAGACCGCGGGCGCCAGCCCCGGCCTG
ACCTTCCTCGAGGCCCTGCGCGCCTGCCCGCTCCCGGTCGTGGCCGCCGTGGAGGGCCACGCGGTCGGCATCGGCACGAC
GCTGCTGCTCCACTGCGACCTCGCCTACGCGGGGGACGGGGCGCGGCTCCGTCTGCCGTTCACGGCCCTGGGCCTCAGTC
CGGAGGGCGGGTCGAGTTACCTGCTGCCCCTGGTGGCGGGATCGAAGCGCGCCGCCGAGCTGCTGATGCTCGGCGAGCCC
TTCACGGCCGCGGACGCCGCCGCGGCCGGCCTGATCAACGCCGCCGTCCCGGCCGGGACGGCCCTGGAGACCGCTCTGAC
CAAGGCGCGCGCGCTCGCCGCCCTGCCCCGGGTCTCGATCGCCGCCACCAAGCAGGCCCTGCGCCGCGGCCACGACGACA
GCGTCGCCCGCGCCCTCGACGCGGAGGCGGAGGTCTTCCACGCCCTCCGCCGCGGTCCGGCGGCGCAGGCGGCCTTCGCG
GCGTTCCTGCGCCGATGA

Upstream 100 bases:

>100_bases
ACCTCGTCGCCCGCCTCGCCCGGGAATACGCGGCGGCGCGGGCGCGGACGGCCGGCAGCGCCGCGGACTACGCGACGGAC
CGCGCCGGCGGGGTCGCCCC

Downstream 100 bases:

>100_bases
GCGGGCCGATGGAGGCGGATGCGGGCCTGCGCACCGGATCCGACGGCGCCGAGCCCGACGCTGGCGGGTCCGACGCAGGC
GGGTCCGGCGCGAAGGAGGA

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSVRVADPEPGIRLVTMDRPERRNALDRATYAGLTDALDTAGRDAEVRALVLTGAGGCFTAGNDLKDFQDVETAGASPGL
TFLEALRACPLPVVAAVEGHAVGIGTTLLLHCDLAYAGDGARLRLPFTALGLSPEGGSSYLLPLVAGSKRAAELLMLGEP
FTAADAAAAGLINAAVPAGTALETALTKARALAALPRVSIAATKQALRRGHDDSVARALDAEAEVFHALRRGPAAQAAFA
AFLRR

Sequences:

>Translated_245_residues
MSVRVADPEPGIRLVTMDRPERRNALDRATYAGLTDALDTAGRDAEVRALVLTGAGGCFTAGNDLKDFQDVETAGASPGL
TFLEALRACPLPVVAAVEGHAVGIGTTLLLHCDLAYAGDGARLRLPFTALGLSPEGGSSYLLPLVAGSKRAAELLMLGEP
FTAADAAAAGLINAAVPAGTALETALTKARALAALPRVSIAATKQALRRGHDDSVARALDAEAEVFHALRRGPAAQAAFA
AFLRR
>Mature_244_residues
SVRVADPEPGIRLVTMDRPERRNALDRATYAGLTDALDTAGRDAEVRALVLTGAGGCFTAGNDLKDFQDVETAGASPGLT
FLEALRACPLPVVAAVEGHAVGIGTTLLLHCDLAYAGDGARLRLPFTALGLSPEGGSSYLLPLVAGSKRAAELLMLGEPF
TAADAAAAGLINAAVPAGTALETALTKARALAALPRVSIAATKQALRRGHDDSVARALDAEAEVFHALRRGPAAQAAFAA
FLRR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI45643119, Length=249, Percent_Identity=32.9317269076305, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI260275230, Length=249, Percent_Identity=32.9317269076305, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI260274832, Length=249, Percent_Identity=32.9317269076305, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI194097323, Length=217, Percent_Identity=30.8755760368664, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI70995211, Length=237, Percent_Identity=30.8016877637131, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI4757968, Length=234, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI49355787, Length=234, Percent_Identity=27.7777777777778, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4757966, Length=234, Percent_Identity=27.3504273504274, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI51538581, Length=234, Percent_Identity=27.3504273504274, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI25453481, Length=234, Percent_Identity=27.3504273504274, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI51538573, Length=234, Percent_Identity=27.3504273504274, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI221136756, Length=235, Percent_Identity=25.1063829787234, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221136753, Length=235, Percent_Identity=25.1063829787234, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI221307494, Length=230, Percent_Identity=25.6521739130435, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=27.6923076923077, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI116235487, Length=193, Percent_Identity=27.4611398963731, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI150378541, Length=227, Percent_Identity=29.9559471365639, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4502327, Length=228, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=153, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157694516, Length=247, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI213417737, Length=247, Percent_Identity=23.0769230769231, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=249, Percent_Identity=32.5301204819277, Blast_Score=117, Evalue=5e-28,
Organism=Escherichia coli, GI1787660, Length=252, Percent_Identity=34.5238095238095, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1788597, Length=214, Percent_Identity=28.9719626168224, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI221142681, Length=208, Percent_Identity=30.7692307692308, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1790281, Length=184, Percent_Identity=31.5217391304348, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=190, Percent_Identity=31.0526315789474, Blast_Score=67, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=242, Percent_Identity=30.5785123966942, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17549921, Length=204, Percent_Identity=33.8235294117647, Blast_Score=111, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17554946, Length=230, Percent_Identity=29.1304347826087, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=237, Percent_Identity=29.535864978903, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=29.0909090909091, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560910, Length=202, Percent_Identity=28.2178217821782, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=234, Percent_Identity=25.2136752136752, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=238, Percent_Identity=22.6890756302521, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323314, Length=249, Percent_Identity=26.9076305220884, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=235, Percent_Identity=31.4893617021277, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI20129971, Length=232, Percent_Identity=30.6034482758621, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24653477, Length=232, Percent_Identity=30.6034482758621, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19920382, Length=234, Percent_Identity=29.0598290598291, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21357171, Length=229, Percent_Identity=29.2576419213974, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653139, Length=221, Percent_Identity=29.4117647058824, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922422, Length=219, Percent_Identity=25.1141552511416, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24650670, Length=211, Percent_Identity=29.3838862559242, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 25175; Mature: 25044

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRVADPEPGIRLVTMDRPERRNALDRATYAGLTDALDTAGRDAEVRALVLTGAGGCFT
CCEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
AGNDLKDFQDVETAGASPGLTFLEALRACPLPVVAAVEGHAVGIGTTLLLHCDLAYAGDG
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEEECCEEEEEEEEEEECCCC
ARLRLPFTALGLSPEGGSSYLLPLVAGSKRAAELLMLGEPFTAADAAAAGLINAAVPAGT
CEEEECEEEECCCCCCCCCEEEEEECCCHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCH
ALETALTKARALAALPRVSIAATKQALRRGHDDSVARALDAEAEVFHALRRGPAAQAAFA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AFLRR
HHHCC
>Mature Secondary Structure 
SVRVADPEPGIRLVTMDRPERRNALDRATYAGLTDALDTAGRDAEVRALVLTGAGGCFT
CEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCC
AGNDLKDFQDVETAGASPGLTFLEALRACPLPVVAAVEGHAVGIGTTLLLHCDLAYAGDG
CCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECCCCEEECCEEEEEEEEEEECCCC
ARLRLPFTALGLSPEGGSSYLLPLVAGSKRAAELLMLGEPFTAADAAAAGLINAAVPAGT
CEEEECEEEECCCCCCCCCEEEEEECCCHHHHHEEEECCCCCHHHHHHHHHHHHCCCCCH
ALETALTKARALAALPRVSIAATKQALRRGHDDSVARALDAEAEVFHALRRGPAAQAAFA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
AFLRR
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]