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The map label for this gene is ilvX [H]

Identifier: 170747332

GI number: 170747332

Start: 942035

End: 943648

Strand: Reverse

Name: ilvX [H]

Synonym: Mrad2831_0899

Alternate gene names: 170747332

Gene position: 943648-942035 (Counterclockwise)

Preceding gene: 170747335

Following gene: 170747331

Centisome position: 15.53

GC content: 73.48

Gene sequence:

>1614_bases
ATGCGAGTCGCCAGCCGGGACGGCCGGCCCCTGAAGGAGACGCCCGTGACCGACGAGACGCCGCGCGCCATGAACGGGGC
CGAGAGCTTGGTCCGGACCCTGGTGGAGGGCGGCGTCGAGGTCTGCTTCACCAATCCCGGCACCTCCGAGATGCACTTCG
TCGCCGCCCTCGACCGCGTCGAGGGCATGCGCTGCGTCCTGTTCCTGTTCGAGGGCGGGGCGACCGGCGCCGCCGACGGC
TACGCCCGCATGGCCGACAAGCCGGCCTCGACCCTGCTCCATCTCGGGCCCGGCCTCGCCAACGGGCTGGCCAACCTCCA
CAACGCCAAGAAGGCGCGCACGCCCGTCGTCAACATCGTCGGCGAGCACGCGACCTACCACCGGGAGTACAACGCGCCGC
TCACCTCGGACATCGAGGGGCTGGCCCACCCGATGTCCTCCTGGGTGCGGACCGGCATGAGCGCGACCGAGATCGGCTCC
GACGGCGCCGCCGCCATCGCGGCCGCCCGCACCGCCCCGGGTCAGGTCGCGACCCTGATCCTGCCGGCCAACACCGCCTG
GGATCCCGGCACCGGCCCCGAGCCGGTGCCGCCGATCCCCGAGCGGGCGCGGGCGAGCGACGACGCGATCGCGGCGGCCG
TGCGGGCCCTGCAGAGCGGCGAGCCCGTGCTCCTGCATCTCGGCGACCGGGCCGTGCGCGGCGAGGGCCGGGTGATCGCC
GACCGGATCGCCCGCAAGACCGGCGCCCGGCTGATGGCGATGACCTCGAACGCCCGGATCGACCGCGGCGCCGGCACCGT
GCCGATCGAGCGCCTCCCCTACCCGATCGACCAGGGGATCGCGGCGCTCAAGGACTTCCGCCACATCGTCCTGATCGGCG
CGACCCCGCCGGTCGGCTTCTTCGCCTACCCGGGCAAGCCGAGCCTGCTGGCGCCGGAGGGCAGCACCACGATCACGCTG
GCCACGCCCGAGGAGGACCAGATCGACGCCCTGGAGCGGCTCGCCGAGGCCGTCGCGGCCCCGCCCGCCGAGCCGGTCGC
TGTCGGGCCGCGGCCGAAGCTCCCGACCTCCGGTCCCCTCGACCAGGACACGATCGGGCAGGTGCTCGGCGCGCTGATCC
CCGAGGGCGCGGTGATCTGCGACGAGTCGATCACCACGGGCCGCAATTTCTTCGCCCTGACCCGGGACGCCGCGCCCCAC
ACCTGGCTCCAGCTCAGCGGCGGCTCGATCGGCCTCGGCATCCCGATGGCGACCGGCGCGGCCGTGGCCTGCCCCGACCG
CAAGGTCATCAACCTGCAGGCCGATGGCAGCGGGCTCTACACGGTGCAGGCGCTCTGGACCCAGGCGCGCGAGCACCTCG
ACGTGGTGACGCTCGTCTGGTCGAACCGCTCCTACGCGATCCTGCGGGCGGAACTCACCAATGTCGGCGCGCTCAATCCC
GGCCCGAAGGCGCTGGGCATGCTGAGCCTCGACGATCCGGCCGTGGATTGGGTGAGCCTCGCCCGCGGCTTCGGCGTCGC
GGGGCGGCGGGTCGAGACCCTGCCCGACTTCATCGAGGCGTTCCAGCACGCGCTGGCCAACCCGGGACCGCACCTCATCG
AAGTGGCTCTGTGA

Upstream 100 bases:

>100_bases
TTCCCGCGCCACGCCCAGGCTTAGGCCCTGCGTTCGGCGAACAGAAGATGCCGGACGTCGCCTCTTGCGCGTGGCGCCGG
CTGCGTCACGATAATACAAA

Downstream 100 bases:

>100_bases
GCGTGGCGACCTACGCTCTCCCCGGCCTCGACGGGCCGGTGGAGATCCGCGTCGACCGCTGGGGGCTGGCGCATATCCGC
GCCGGCACCGCGCTCGACGC

Product: hypothetical protein

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MRVASRDGRPLKETPVTDETPRAMNGAESLVRTLVEGGVEVCFTNPGTSEMHFVAALDRVEGMRCVLFLFEGGATGAADG
YARMADKPASTLLHLGPGLANGLANLHNAKKARTPVVNIVGEHATYHREYNAPLTSDIEGLAHPMSSWVRTGMSATEIGS
DGAAAIAAARTAPGQVATLILPANTAWDPGTGPEPVPPIPERARASDDAIAAAVRALQSGEPVLLHLGDRAVRGEGRVIA
DRIARKTGARLMAMTSNARIDRGAGTVPIERLPYPIDQGIAALKDFRHIVLIGATPPVGFFAYPGKPSLLAPEGSTTITL
ATPEEDQIDALERLAEAVAAPPAEPVAVGPRPKLPTSGPLDQDTIGQVLGALIPEGAVICDESITTGRNFFALTRDAAPH
TWLQLSGGSIGLGIPMATGAAVACPDRKVINLQADGSGLYTVQALWTQAREHLDVVTLVWSNRSYAILRAELTNVGALNP
GPKALGMLSLDDPAVDWVSLARGFGVAGRRVETLPDFIEAFQHALANPGPHLIEVAL

Sequences:

>Translated_537_residues
MRVASRDGRPLKETPVTDETPRAMNGAESLVRTLVEGGVEVCFTNPGTSEMHFVAALDRVEGMRCVLFLFEGGATGAADG
YARMADKPASTLLHLGPGLANGLANLHNAKKARTPVVNIVGEHATYHREYNAPLTSDIEGLAHPMSSWVRTGMSATEIGS
DGAAAIAAARTAPGQVATLILPANTAWDPGTGPEPVPPIPERARASDDAIAAAVRALQSGEPVLLHLGDRAVRGEGRVIA
DRIARKTGARLMAMTSNARIDRGAGTVPIERLPYPIDQGIAALKDFRHIVLIGATPPVGFFAYPGKPSLLAPEGSTTITL
ATPEEDQIDALERLAEAVAAPPAEPVAVGPRPKLPTSGPLDQDTIGQVLGALIPEGAVICDESITTGRNFFALTRDAAPH
TWLQLSGGSIGLGIPMATGAAVACPDRKVINLQADGSGLYTVQALWTQAREHLDVVTLVWSNRSYAILRAELTNVGALNP
GPKALGMLSLDDPAVDWVSLARGFGVAGRRVETLPDFIEAFQHALANPGPHLIEVAL
>Mature_537_residues
MRVASRDGRPLKETPVTDETPRAMNGAESLVRTLVEGGVEVCFTNPGTSEMHFVAALDRVEGMRCVLFLFEGGATGAADG
YARMADKPASTLLHLGPGLANGLANLHNAKKARTPVVNIVGEHATYHREYNAPLTSDIEGLAHPMSSWVRTGMSATEIGS
DGAAAIAAARTAPGQVATLILPANTAWDPGTGPEPVPPIPERARASDDAIAAAVRALQSGEPVLLHLGDRAVRGEGRVIA
DRIARKTGARLMAMTSNARIDRGAGTVPIERLPYPIDQGIAALKDFRHIVLIGATPPVGFFAYPGKPSLLAPEGSTTITL
ATPEEDQIDALERLAEAVAAPPAEPVAVGPRPKLPTSGPLDQDTIGQVLGALIPEGAVICDESITTGRNFFALTRDAAPH
TWLQLSGGSIGLGIPMATGAAVACPDRKVINLQADGSGLYTVQALWTQAREHLDVVTLVWSNRSYAILRAELTNVGALNP
GPKALGMLSLDDPAVDWVSLARGFGVAGRRVETLPDFIEAFQHALANPGPHLIEVAL

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=560, Percent_Identity=23.0357142857143, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 56242; Mature: 56242

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVASRDGRPLKETPVTDETPRAMNGAESLVRTLVEGGVEVCFTNPGTSEMHFVAALDRV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
EGMRCVLFLFEGGATGAADGYARMADKPASTLLHLGPGLANGLANLHNAKKARTPVVNIV
CCCEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEE
GEHATYHREYNAPLTSDIEGLAHPMSSWVRTGMSATEIGSDGAAAIAAARTAPGQVATLI
CCCHHEHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHEECCCCCCCEEEEE
LPANTAWDPGTGPEPVPPIPERARASDDAIAAAVRALQSGEPVLLHLGDRAVRGEGRVIA
EECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCHH
DRIARKTGARLMAMTSNARIDRGAGTVPIERLPYPIDQGIAALKDFRHIVLIGATPPVGF
HHHHHHHCCEEEEEECCCCEECCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCE
FAYPGKPSLLAPEGSTTITLATPEEDQIDALERLAEAVAAPPAEPVAVGPRPKLPTSGPL
EECCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
DQDTIGQVLGALIPEGAVICDESITTGRNFFALTRDAAPHTWLQLSGGSIGLGIPMATGA
CHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCEEEEECCCEEEECCCCCCCC
AVACPDRKVINLQADGSGLYTVQALWTQAREHLDVVTLVWSNRSYAILRAELTNVGALNP
EEECCCCEEEEEEECCCCEEHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCC
GPKALGMLSLDDPAVDWVSLARGFGVAGRRVETLPDFIEAFQHALANPGPHLIEVAL
CCHHEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure
MRVASRDGRPLKETPVTDETPRAMNGAESLVRTLVEGGVEVCFTNPGTSEMHFVAALDRV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHH
EGMRCVLFLFEGGATGAADGYARMADKPASTLLHLGPGLANGLANLHNAKKARTPVVNIV
CCCEEEEEEEECCCCCCCCCHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEE
GEHATYHREYNAPLTSDIEGLAHPMSSWVRTGMSATEIGSDGAAAIAAARTAPGQVATLI
CCCHHEHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHEECCCCCCCEEEEE
LPANTAWDPGTGPEPVPPIPERARASDDAIAAAVRALQSGEPVLLHLGDRAVRGEGRVIA
EECCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEEEEECCEEECCCCCCHH
DRIARKTGARLMAMTSNARIDRGAGTVPIERLPYPIDQGIAALKDFRHIVLIGATPPVGF
HHHHHHHCCEEEEEECCCCEECCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCE
FAYPGKPSLLAPEGSTTITLATPEEDQIDALERLAEAVAAPPAEPVAVGPRPKLPTSGPL
EECCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCC
DQDTIGQVLGALIPEGAVICDESITTGRNFFALTRDAAPHTWLQLSGGSIGLGIPMATGA
CHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCCCCEEEEECCCEEEECCCCCCCC
AVACPDRKVINLQADGSGLYTVQALWTQAREHLDVVTLVWSNRSYAILRAELTNVGALNP
EEECCCCEEEEEEECCCCEEHHHHHHHHHHHCCEEEEEEECCCCEEEEEEECCCCCCCCC
GPKALGMLSLDDPAVDWVSLARGFGVAGRRVETLPDFIEAFQHALANPGPHLIEVAL
CCHHEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]