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The map label for this gene is pit [H]

Identifier: 170747209

GI number: 170747209

Start: 815760

End: 816746

Strand: Reverse

Name: pit [H]

Synonym: Mrad2831_0775

Alternate gene names: 170747209

Gene position: 816746-815760 (Counterclockwise)

Preceding gene: 170747210

Following gene: 170747204

Centisome position: 13.44

GC content: 71.23

Gene sequence:

>987_bases
GTGACGCTCCTCGTCGTCCTCATCGTCCTCGCCCTGGTCTTCGACTTCCTGAACGGGCTGCACGACGCCGCCAACTCCAT
CGCCACCATCGTGTCGACGCGGGTGCTGGCGCCCCGCTACGCCGTGTTCTGGGCGGCGTTCTTCAACTTCGTCGCCTTCC
TGGTCTTCGGCCTGCACGTCGCCGGCACGGTGGGCTCGGGCATCATCGACGTCGCCACGGTGGACGACCGGGTCATCATG
GGCGCCCTCGGCGGCGCCATCACGTGGAACCTCGTCACGTGGTACGCCGGGATCCCGTCCTCCAGCTCCCACGCCCTGAT
CGGCGGCCTGCTGGGCGCCGGCATCGCCAAGGCGGGAGTCGGCGTGATCGTCTGGCCCGGCGTCATCGCCACCAGCGCCG
CCATCGTCCTCTCGCCGGCCCTCGGCTTCGCCCTGGGGCTGCTGCTGATGCTGGCCGTCTCGTGGATCTTCGTCCGCGCG
ACGCCGCACGCCGTCGACCGCCTGTTCCGGGGCCTGCAATTCGTCTCGGCCTCGCTCTACTCGCTCGGCCACGGCGGCAA
CGACGCCCAGAAGACCATGGGCATCATCGCGGCCCTGCTCTACGCTCACGGCGAGAGCGGGGCCTTCCACGTGCCGCTCT
GGGTGGTGCTGTCCTGCCAGACCGCCATGGCCCTCGGCACCCTTCTGGGCGGCTGGCGCATCGTCCACACGATGGGCTCG
AAGATCACGCGGCTGTCGCCCATGCAGGGCTTCTGCGCCGAGACCGGCGGCGCCGCGACGCTGTTCGCCGCGACCGCCTT
CGGCATCCCGGTCTCGACCACCCACACGATCACGGGCGCCATCGTCGGCGTCGGCGCGGCGCGGCGGGTCTCGGCGGTCC
GCTGGAACGTCGCGCAGGGCATCGTGATCGCCTGGGTGATCACGATGCCGGCCGCGGCCCTGGTCGCGGCCCTGACCTAC
GCGGCGTCGGGGCTCGTCCTGCCATAG

Upstream 100 bases:

>100_bases
ACTGTACGGCCATCTGGAGAAGGTCATGGACCGGTTCGAGGACGTGGCGAACCAGATCAGCAGCATCGTCGTCGAACATG
TCTGAGCCGCAGGTCGCCGC

Downstream 100 bases:

>100_bases
GGGACGGGCGCGGCCGGATCCGGCACCGGTTCACCAGCGATGGCGCCAGCCGCCATGATGCCAGCCGCCGTGCCATCCCC
CATGCCAGCCGGCATGGCGG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MTLLVVLIVLALVFDFLNGLHDAANSIATIVSTRVLAPRYAVFWAAFFNFVAFLVFGLHVAGTVGSGIIDVATVDDRVIM
GALGGAITWNLVTWYAGIPSSSSHALIGGLLGAGIAKAGVGVIVWPGVIATSAAIVLSPALGFALGLLLMLAVSWIFVRA
TPHAVDRLFRGLQFVSASLYSLGHGGNDAQKTMGIIAALLYAHGESGAFHVPLWVVLSCQTAMALGTLLGGWRIVHTMGS
KITRLSPMQGFCAETGGAATLFAATAFGIPVSTTHTITGAIVGVGAARRVSAVRWNVAQGIVIAWVITMPAAALVAALTY
AASGLVLP

Sequences:

>Translated_328_residues
MTLLVVLIVLALVFDFLNGLHDAANSIATIVSTRVLAPRYAVFWAAFFNFVAFLVFGLHVAGTVGSGIIDVATVDDRVIM
GALGGAITWNLVTWYAGIPSSSSHALIGGLLGAGIAKAGVGVIVWPGVIATSAAIVLSPALGFALGLLLMLAVSWIFVRA
TPHAVDRLFRGLQFVSASLYSLGHGGNDAQKTMGIIAALLYAHGESGAFHVPLWVVLSCQTAMALGTLLGGWRIVHTMGS
KITRLSPMQGFCAETGGAATLFAATAFGIPVSTTHTITGAIVGVGAARRVSAVRWNVAQGIVIAWVITMPAAALVAALTY
AASGLVLP
>Mature_327_residues
TLLVVLIVLALVFDFLNGLHDAANSIATIVSTRVLAPRYAVFWAAFFNFVAFLVFGLHVAGTVGSGIIDVATVDDRVIMG
ALGGAITWNLVTWYAGIPSSSSHALIGGLLGAGIAKAGVGVIVWPGVIATSAAIVLSPALGFALGLLLMLAVSWIFVRAT
PHAVDRLFRGLQFVSASLYSLGHGGNDAQKTMGIIAALLYAHGESGAFHVPLWVVLSCQTAMALGTLLGGWRIVHTMGSK
ITRLSPMQGFCAETGGAATLFAATAFGIPVSTTHTITGAIVGVGAARRVSAVRWNVAQGIVIAWVITMPAAALVAALTYA
ASGLVLP

Specific function: Low-affinity inorganic phosphate transport (Probable) [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=164, Percent_Identity=34.1463414634146, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI5803173, Length=153, Percent_Identity=35.2941176470588, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1789907, Length=220, Percent_Identity=32.7272727272727, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789360, Length=224, Percent_Identity=31.6964285714286, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=31.4814814814815, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=30.8641975308642, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17539280, Length=160, Percent_Identity=32.5, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17536725, Length=176, Percent_Identity=27.8409090909091, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32566716, Length=171, Percent_Identity=29.8245614035088, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17557328, Length=153, Percent_Identity=31.3725490196078, Blast_Score=75, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319773, Length=160, Percent_Identity=31.875, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356511, Length=187, Percent_Identity=28.3422459893048, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 33716; Mature: 33585

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLVVLIVLALVFDFLNGLHDAANSIATIVSTRVLAPRYAVFWAAFFNFVAFLVFGLHV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AGTVGSGIIDVATVDDRVIMGALGGAITWNLVTWYAGIPSSSSHALIGGLLGAGIAKAGV
HHHHCCCEEEEEECCCHHHHHHHCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCC
GVIVWPGVIATSAAIVLSPALGFALGLLLMLAVSWIFVRATPHAVDRLFRGLQFVSASLY
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLGHGGNDAQKTMGIIAALLYAHGESGAFHVPLWVVLSCQTAMALGTLLGGWRIVHTMGS
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KITRLSPMQGFCAETGGAATLFAATAFGIPVSTTHTITGAIVGVGAARRVSAVRWNVAQG
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
IVIAWVITMPAAALVAALTYAASGLVLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLLVVLIVLALVFDFLNGLHDAANSIATIVSTRVLAPRYAVFWAAFFNFVAFLVFGLHV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHH
AGTVGSGIIDVATVDDRVIMGALGGAITWNLVTWYAGIPSSSSHALIGGLLGAGIAKAGV
HHHHCCCEEEEEECCCHHHHHHHCCHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCC
GVIVWPGVIATSAAIVLSPALGFALGLLLMLAVSWIFVRATPHAVDRLFRGLQFVSASLY
CEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
SLGHGGNDAQKTMGIIAALLYAHGESGAFHVPLWVVLSCQTAMALGTLLGGWRIVHTMGS
HHCCCCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KITRLSPMQGFCAETGGAATLFAATAFGIPVSTTHTITGAIVGVGAARRVSAVRWNVAQG
HHHHCCCCHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHH
IVIAWVITMPAAALVAALTYAASGLVLP
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9696772; 11481430 [H]