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The map label for this gene is dgoD [H]

Identifier: 170684206

GI number: 170684206

Start: 2423517

End: 2424719

Strand: Reverse

Name: dgoD [H]

Synonym: EcSMS35_2380

Alternate gene names: 170684206

Gene position: 2424719-2423517 (Counterclockwise)

Preceding gene: 170683353

Following gene: 170681961

Centisome position: 47.84

GC content: 40.81

Gene sequence:

>1203_bases
ATGAAGATTACATCTGTAGAAATTTTTGACTGCGAACTCAAAAAAAGAGATCAAACTATGGCCTCTTATAATCCAGTACT
TATTCGGGTTAATACCGATGCTGGAATAAGTGGTGTTGGCGAAGTAGGATTAGCCTATGGTGCAGGTGCAAAATCTGGTG
TTGGTATTATCAGAGATTTAGCCCCTCTCATCATTGGTGAAGATCCGTTGAACATTGAGAAGATTTGGGAATTCCTCTTC
AGGAAAACATTTTGGGGAATGGGCGGTGGTAATGTTTTTTACGCAGGTATGAGCGCTATTGATATTGCCTTATGGGATAT
TAAAGGTAAATATTTAAACGTGCCGGTTTATCAACTACTGGGGGGAAAGACTAACGATAAATTGAGAACTTACGCCAGTC
AGTTACAATTTGGTTGGGGCGAAAAAAGGCAAATATTAGTAACGCCTGAAGAGTATGCAGAGGCAGCGCGTGCCGCTATT
GCAGAAGGTTATAATGCGATAAAAGTTGATCCGCTTGAAATCGATCTGCATGGCGGCGATTGTGTCTTTCAGAACAAAAA
TAGAAATTACTCTGGCTTATTACTGGCCGAACAATTAAAAATGGGCGAGGCAAGAATTGCCGCTATGCGTGAAGCAATGG
GCGACGATGCTGATATTATTGTAGAGATTCATTCTCTTCTAGGAACAAATTCAGCGATCCAGTTTGCCAAAGCAATAGAG
AAATATCGTATTTTTCTCTATGAGGAGCCAATTCATCCATTAAATTCGGATAATATGCAGAAAGTTGCCCGTTCAACATC
GATTCCGATAGCAACAGGTGAGCGTTCTTATACTCGCTGGGGATATCGTGATTTACTGGAAAAACAATCAATTGCGGTAG
CTCAACCTGATTTGTGTCTTTGTGGTGGCATTACAGAGGGAAAGAAAATTTGTGATTATGCCAATATATATGACACAACC
GTTCAGGTACATGTTTGTGGCGGGCCTGTTTCAACAGTAGCGGCGCTGCATATGGAAACTGCAATACCTAACTTTATTAT
TCATGAACATCATACCAATGCGATGAAAGCATGTATTCGGGAACTTTGTACTTACGATTATCAACCCGAGAATGGCTATT
ATGTCGCGCCAGAGTTGCCTGGGCTTGGTCAGGAATTAAACGATGAAATCGTACAACAATATCTTGCCTATGTGATCAAA
TAA

Upstream 100 bases:

>100_bases
TATCGCCGTAGCTATTTTGTTATCAATGAAAATTGATAATAAAAAAGATAACGCCCAGAAAGACAATGTTATTTCACATG
CTAAATAAATGAGGTTATTT

Downstream 100 bases:

>100_bases
CGGCGATTTATATACTCTAAATAATTCGAGTTGCAGGAAGGCGACAAGCGAGTGACAAATTCGTCAGGAACGAATTTGCC
CAGCCGCAGGCTGGCCTTCG

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK

Sequences:

>Translated_400_residues
MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK
>Mature_400_residues
MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=375, Percent_Identity=28.5333333333333, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787864, Length=407, Percent_Identity=28.992628992629, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI226510960, Length=362, Percent_Identity=25.414364640884, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44318; Mature: 44318

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDL
CCEEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCCCCCHHHHHH
APLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCCHHHHH
GGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAIAEGYNAIKVDPLEIDLHGGD
CCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECEEEEEECCCE
CVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
EEEECCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHHHHHH
KYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCL
HHEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCHHHCCHHHHHHHCCCEECCCCEEE
CGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIR
ECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
ELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK
HHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDAGISGVGEVGLAYGAGAKSGVGIIRDL
CCEEEEEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHEECCCCCCCCHHHHHH
APLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLNVPVYQLL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCCHHHHH
GGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAIAEGYNAIKVDPLEIDLHGGD
CCCCCHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECEEEEEECCCE
CVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
EEEECCCCCCCEEEEHHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHHHHHH
KYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERSYTRWGYRDLLEKQSIAVAQPDLCL
HHEEEEEECCCCCCCCCHHHHHHHHCCCCEECCCCCHHHCCHHHHHHHCCCEECCCCEEE
CGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKACIR
ECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
ELCTYDYQPENGYYVAPELPGLGQELNDEIVQQYLAYVIK
HHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA