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The map label for this gene is serA [H]

Identifier: 170684118

GI number: 170684118

Start: 3030901

End: 3031848

Strand: Reverse

Name: serA [H]

Synonym: EcSMS35_2958

Alternate gene names: 170684118

Gene position: 3031848-3030901 (Counterclockwise)

Preceding gene: 170680141

Following gene: 170681128

Centisome position: 59.82

GC content: 44.2

Gene sequence:

>948_bases
ATGAAAAATGTTCTGGTCACCGTCCCCAGCTTTTCAGCACGCTGTGTTTCCGCCAGCAAGTTGCTAAGAGAGAATAATTT
TAATCTTATTATTAAGAATAATGTGGAGCATCTGCTTAAATCAGAATCCACTGCATTACGAGAATCTATTTGCGCGGTCA
TCGCCGGAAAAGATTGTTATCAGGCGGACACGCTATCATTGCTGTCTGGTGTGAAAATAATATCGAGATTCGGGACGGGG
ATAGACAATATTGATCTTCGTGCGGCACAGCAATCCGGTATTGTTGTCAATAATGCGGTTGGGATTAATTCAAATGCGGT
TGCGGAATTTATCATTGGACTTATCTTCGCGAGCATGAGAAATATCCCCGGTAGCTATCATGCGATGCAAAATGGTTATT
GGGGCGAGTCGCATGGCTGCGAGTTACAAGGTAAACGTATTGGCCTGCTTGGCTACGGCAATATTGGCAAAACACTGGCG
AAAAGGTTATCTGGTTTTGATGTTGAGCTTTTAGCTTTTGACAAACAACCCGATTTCCAGGCCGCAGGCAAAGATGGAGT
TCAGTTCGTATCAATTGAAGATATCTTTATGCAGTCTCATGTCATCATCGTTCTTTTGCCATTTTCTCCGGAGCTGGAGA
ACTTCATTAGTCATAAATATTTGTCGATGATGCGCAATGGTGCTCTGATCATCAACGCCGCAAGGGGAAAGTTGCTGGAT
GAGGGTGCTTTACTTCAGGTAATTGAAGAACGAAATGTTTTTGCAGCACTGGATGTGTTTAGCTCTGAACCGCTAGCGCA
ATTTAGCCCATTGCTACATGCCAAGAATATCATTACAACGCCGCATATTGCGGCCGCCACCGTTGAGTCATACCAGCAAA
CCGGGATATACGTTGCCCAGTCGATAATTGATTACTTCGCAGGAAGGGAGATAAAAAACGTGCTGTGA

Upstream 100 bases:

>100_bases
GTGCGCTCGGTATTAAAGCAAACTCATAATTTCAAAATATAAATACAGTAACCGGTCACGCCATCTTTTGGGGTGGCGTT
CTTTGAAGTGAGTGTCAGCT

Downstream 100 bases:

>100_bases
GTTCAGCTAAGGAATATTTCAGGTACAAAAAAACCGCAATTTGTGAAACACATTGCGGTTTATCAGCATCATAAGTGTTG
GTTGCGGGGGCCGGATTTGA

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: PGDH [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSESTALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTG
IDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLD
EGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIYVAQSIIDYFAGREIKNVL

Sequences:

>Translated_315_residues
MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSESTALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTG
IDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLD
EGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIYVAQSIIDYFAGREIKNVL
>Mature_315_residues
MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSESTALRESICAVIAGKDCYQADTLSLLSGVKIISRFGTG
IDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLA
KRLSGFDVELLAFDKQPDFQAAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLD
EGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIYVAQSIIDYFAGREIKNVL

Specific function: Serine biosynthesis; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=300, Percent_Identity=31, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI6912396, Length=254, Percent_Identity=28.3464566929134, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI145580575, Length=252, Percent_Identity=30.952380952381, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI145580578, Length=278, Percent_Identity=29.4964028776978, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4557499, Length=278, Percent_Identity=29.4964028776978, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI4557497, Length=277, Percent_Identity=28.5198555956679, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI61743967, Length=277, Percent_Identity=28.5198555956679, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789279, Length=239, Percent_Identity=32.2175732217573, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI87082289, Length=258, Percent_Identity=26.7441860465116, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787645, Length=197, Percent_Identity=31.9796954314721, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI87081824, Length=238, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=297, Percent_Identity=29.2929292929293, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=28.2685512367491, Blast_Score=84, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6322116, Length=245, Percent_Identity=30.6122448979592, Blast_Score=115, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=248, Percent_Identity=30.241935483871, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=237, Percent_Identity=27.4261603375527, Blast_Score=107, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=265, Percent_Identity=26.0377358490566, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321253, Length=160, Percent_Identity=29.375, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921140, Length=248, Percent_Identity=32.258064516129, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24585516, Length=323, Percent_Identity=26.9349845201238, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI28574286, Length=295, Percent_Identity=25.4237288135593, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45552429, Length=249, Percent_Identity=24.8995983935743, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45551003, Length=249, Percent_Identity=24.8995983935743, Blast_Score=110, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24585514, Length=249, Percent_Identity=24.8995983935743, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574282, Length=249, Percent_Identity=24.8995983935743, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28574284, Length=249, Percent_Identity=24.8995983935743, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI28571528, Length=281, Percent_Identity=27.7580071174377, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24646446, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646448, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646452, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646450, Length=274, Percent_Identity=28.8321167883212, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI62472511, Length=274, Percent_Identity=28.8321167883212, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR006236
- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR015508
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C; PF01842 ACT [H]

EC number: =1.1.1.95 [H]

Molecular weight: Translated: 34349; Mature: 34349

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSESTALRESICAVIAGKDCY
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCHHHHHHHHHHHHHCCCCCC
QADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMR
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
NIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLAKRLSGFDVELLAFDKQPDFQ
CCCCCHHHHCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCC
AAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLD
CCCCCCEEEEEHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
EGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIYVAQ
CCHHHHHHHHCCEEEEEEHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SIIDYFAGREIKNVL
HHHHHHCCCHHHHCC
>Mature Secondary Structure
MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSESTALRESICAVIAGKDCY
CCCEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHCHHHHHHHHHHHHHCCCCCC
QADTLSLLSGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMR
HHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHH
NIPGSYHAMQNGYWGESHGCELQGKRIGLLGYGNIGKTLAKRLSGFDVELLAFDKQPDFQ
CCCCCHHHHCCCCCCCCCCCEECCCEEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCC
AAGKDGVQFVSIEDIFMQSHVIIVLLPFSPELENFISHKYLSMMRNGALIINAARGKLLD
CCCCCCEEEEEHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCC
EGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIYVAQ
CCHHHHHHHHCCEEEEEEHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
SIIDYFAGREIKNVL
HHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]