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The map label for this gene is smf [H]

Identifier: 170683496

GI number: 170683496

Start: 3786095

End: 3787297

Strand: Reverse

Name: smf [H]

Synonym: EcSMS35_3717

Alternate gene names: 170683496

Gene position: 3787297-3786095 (Counterclockwise)

Preceding gene: 170684175

Following gene: 170680367

Centisome position: 74.72

GC content: 50.21

Gene sequence:

>1203_bases
ATGAATCTTTCAGCCAATGCACAAGCGACCCTCCTGCTAACCAGCGATTTTTCTCGCGCGGCGGCGAGTGAGTATAAACC
TCTTAGTAATAGTGAATGGGGGAAGTTTGCATTATGGCTGAAGCACCAACGTATCAGTCCCGCCGATCTTCTGGTGCCGC
AACCGCAAGAGAAACTTACAGGCTGGAGCGATCCGCGTATTTCTCAGGAGCGTATTCTTGGCTTGCTGGCGCGTGGTCAT
AGTTTGGCGTTGGCGGTAGATAAGTGGCAACGCGCCGGTTTATGGATCTTAACCCGCGGAGATGCCGATTATCCCGTTCG
CTTGAAAAACCGATTGCGAACGGATGCACCTCCCGTTTTATTTGGCTGCGGGAATAAAGCATTACTGCAAGCGGAAGGTA
TGGCGATTGTTGGCTCGCGAGATGCTCCGACTGCCGATTTGCGCTATACCCAACAACTGGCAGCGAAACTGGCCCAACAG
GGGATTTGCGTTATCTCTGGTGGTGCGCGAGGTATTGATGAATGTGCAATGGCGTCGGCACTGGAGGCCGGGGGAACTGC
CGTTGGCGTATTAGCTGATAGCTTGTTAAAAACGAGTACGTTAGTGAAATGGCGTGAAGGGCTTATAGCAGGCAACCTGG
TGTTGATTTCGCCGTTTTACCCAGAGGTACGTTTCACCGTCGGCAATGCGATGGCGCGAAATAAATATATTTATTGCCTT
GCTGAAAGCGCAATGGTTGTACGTGCGGGAATGACCGGTGGAACGATAACCGGGGCGATGGAGGCATTAAAACATCAGTG
GCTGCCTGTGCAGGTTAAACCAAATCAGGATATGCAATCAGCCAATTCACGATTAGTAGAAAATGGGGCGTCATGGAGTA
CTGAACAGGCTGAGAATGTGACGTTCAGACTGCCAGACGTTACTGGGCTGATGTATGACAAAGCACTCCGTAACGCTCAA
CCAGAATTGTTTTCGCTGCACGAAGATGACGCAAATTACGCAGTGATGCCCTCGCATACCCCTGTCGATTTTTATCAACT
TTTTGTGGCGGAACTGGCGATCCTTGCAAAGGAATCGATAAGTATTGAAAGGCTGGCGTCTTGTACTGGTTTAACCATCG
AACAAATTAGTGTGTGGCTGAACCGCGCAGAAGAAGAGGGAAGGGTTATCCGATTGGGCGAAGGTCATTATCAGTTCAGG
TAA

Upstream 100 bases:

>100_bases
ATTCTGCATGGACGCTGACAGTTCTGACAGCACTGTTACGCCAGGCAGGTTGCCCGACGGTTTATCCTCTTGCCCTTGCG
TCTACCTCGGTAAAAAATTG

Downstream 100 bases:

>100_bases
TAAAACAGGTGCCGCGTCTGGTTGTGTGCAGCACCTGTTTGAACCTTACCCCTTCATCCACATTCGAATGCCGTCGAGGA
ACATCTGAGTTGCCATCATC

Product: putative DNA protecting protein DprA

Products: NA

Alternate protein names: DNA-processing chain A [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQERILGLLARGH
SLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTADLRYTQQLAAKLAQQ
GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCL
AESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENVTFRLPDVTGLMYDKALRNAQ
PELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Sequences:

>Translated_400_residues
MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQERILGLLARGH
SLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTADLRYTQQLAAKLAQQ
GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCL
AESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENVTFRLPDVTGLMYDKALRNAQ
PELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR
>Mature_400_residues
MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLTGWSDPRISQERILGLLARGH
SLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTADLRYTQQLAAKLAQQ
GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCL
AESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENVTFRLPDVTGLMYDKALRNAQ
PELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR

Specific function: Essential for efficient chromosomal DNA transformation but not required for plasmid transformation [H]

COG id: COG0758

COG function: function code LU; Predicted Rossmann fold nucleotide-binding protein involved in DNA uptake

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smf family [H]

Homologues:

Organism=Escherichia coli, GI48994932, Length=156, Percent_Identity=32.0512820512821, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003488 [H]

Pfam domain/function: PF02481 DNA_processg_A [H]

EC number: NA

Molecular weight: Translated: 43785; Mature: 43785

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLT
CCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCHHHCC
GWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVL
CCCCCCCCHHHHHHHHHCCCEEEEEEECHHHCCEEEEECCCCCCCHHHHHHCCCCCCCEE
FGCGNKALLQAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAMASA
EECCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
LEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCL
HHCCCCCHHHHHHHHHHHHHHHHHHHCEEECCEEEEECCCCCEEEEECCHHHCCCEEEEE
AESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENV
ECCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCE
TFRLPDVTGLMYDKALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESI
EEECCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
SIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR
CHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC
>Mature Secondary Structure
MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPADLLVPQPQEKLT
CCCCCCCEEEEEEECCCCHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHCCCCCCHHHCC
GWSDPRISQERILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVL
CCCCCCCCHHHHHHHHHCCCEEEEEEECHHHCCEEEEECCCCCCCHHHHHHCCCCCCCEE
FGCGNKALLQAEGMAIVGSRDAPTADLRYTQQLAAKLAQQGICVISGGARGIDECAMASA
EECCCCEEEEECCEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
LEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCL
HHCCCCCHHHHHHHHHHHHHHHHHHHCEEECCEEEEECCCCCEEEEECCHHHCCCEEEEE
AESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSTEQAENV
ECCEEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCE
TFRLPDVTGLMYDKALRNAQPELFSLHEDDANYAVMPSHTPVDFYQLFVAELAILAKESI
EEECCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCC
SIERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR
CHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7768823; 7542800 [H]