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The map label for this gene is recQ [H]

Identifier: 170684175

GI number: 170684175

Start: 3787297

End: 3789393

Strand: Reverse

Name: recQ [H]

Synonym: EcSMS35_3718

Alternate gene names: 170684175

Gene position: 3789393-3787297 (Counterclockwise)

Preceding gene: 170680129

Following gene: 170683496

Centisome position: 74.77

GC content: 49.12

Gene sequence:

>2097_bases
ATGGAAAAGCACGGAGCTGAATTGCTTTTACAGCGGATGTTAGGAAATACCACCGCTACTTTCAGGGAAGGGCAATGGGA
AGCCATAGATGCAGTGGTTAATCAGCGGCGAAAACTGCTGGTTGTACAGCGCACCGGCTGGGGGAAAAGTGCCGTGTACT
TCATCGCCAGTAAAATCTTCCGCGATCGCGGCGCTGGCACGACAATTATCATCTCTCCTTTGCTTGCCCTGATGCGTAAC
CAGGTTGCCGCCGCTGAACGGTTAGGTATTACGGCTGAAACGTTGAATTCTACAAACAGGGAAGAATGGCAGCGCATTAG
CGATAAGTTGCTGCAAGGTGAGGTCGATTGCTTGCTGGTTTCCCCGGAACGTTTGGCAAACCAGGACTTTATCGAAACGG
TTTTATATCCTATAGCCGATCGTATTGGCTTGCTTGTGGTCGACGAGGCGCACTGCATCTCTGATTGGGGCCACGATTTT
CGCCCGGATTACCGACGTATATTAGATATTTTGCGCCAACTGCCTGCGAATACCCCTGTTCTGGGTACAACCGCGACAGC
GAATAACCGTGTTGTTGAGGATATCCGTCAGCAATTGGGTGACATTGTGATTCAGCGTGGAACGCTGGCTCGCGAAAGTT
TGGCGCTTGATGCCTTAGTCTTAGGAGAACAGTCATCCCGTCTGGCATGGTTAGCAACGGTTATCCCTCAGTTTTCCAAA
TCGGGAATTGTTTATACCCTGACGACTCGCGATGCTGAACTTGTCGCCGAGTGGTTAAGGAAAAATGGTATCAGTGCATT
TGCTTACTACAGCGGCGTGACTTGTGAAGGCGCGGAAGATTCTAATACTGCCAGGGAATATCTGGAGCAGGCACTGCTGG
CAAATAAAATCAAAGTGCTGGTCGCGACGACGGCATTAGGTATGGGGTTTGATAAACCTGATTTAGGTTTCGTCATTCAT
TATCAGATGCCGGGGTCTATTGTCGGTTACTACCAACAGGTGGGGCGTGCCGGGCGTGCTATAGATTCCGCAGTTGGCAT
ATTGCTTTGTGGTGGTGAAGACCGCGCTATTCATCAATTCTTCCGTGAAAGCGCCTTTCCTGCGGAGGCGCAAATTCATG
AAATACTCAACGTACTTAGCGAGAATGACGGTCTTACCCTACGAGGCATTGAACAACGGACGAATCTTCGTTATGGGCAA
ATAGAAAAAGCACTGAAATTATTGGTAGCGGAAAATCCATCGCCTGTGGTGTATACCGAGAAATTATGGCGCAGAACTAT
CGTCAGTTTTTCTCCTGATCATGAACGAATTAACCATTTGATGAATCAGAGAAAAAGTGAACTGGCAGACGTTGAAAGCT
ATATCACGACCAAGGAGTGCAAAATGCAATTTCTGCGCCGTGCACTCGATGAGCCAAGTGCCGAACGTTGTGGTAAATGT
AGCAGTTGTCTTCAGCATCCGTTATTGTCGCCCGACATTGATAGCGGCTTACTCCATGCGGCAAATTTATTTATTAAACA
CGCTGACCTGCCGTTAAATCTCAATAAACAGATGGCAGCTGGGGCTTTTACTCAATACGGATTTAAAGGGAACTTGCCTG
CGGGTTTACAAGGATCTACGGGGCGAATCCTTTCTCGTTGGGGAGATTCCGGGTGGGGAAAGCAGGTAGCACAGGAGAAA
AAAACGGGGCGCTTAAGTGATGAGCTGGTAGAAGCATGTGCGGAAATGGTTTGCCAACGCTGGAATCCGCATCCTGAACC
AACCTGGGTATGCTGCGTTCCTTCATTAAGGCACCTCGACCTGGTTCCTGATTTTGCCCGGCGACTGGCGGCGAAACTTG
GCTTACCTTTTATTGATGCCATTGAAAAAGTCGTGGACAATCCACCGCAGAAAATGCAGCAAAACCGTTTTCACCAGTGT
CAAAATCTCGACGGGGCGTTTGTGATTATCCCTCCTTTGATGCCAGGTCCGGCGTTGCTGGTTGACGATATCGTGGATTC
TGCATGGACGCTGACAGTTCTGACAGCACTGTTACGCCAGGCAGGTTGCCCGACGGTTTATCCTCTTGCCCTTGCGTCTA
CCTCGGTAAAAAATTGA

Upstream 100 bases:

>100_bases
CATTTCTCTTATCTCCTCTCTTCATCTTTTGGATCTCTCTCGCAAATTTACCTGCTAATAATCCGTTCTAATCACTGCCT
AAAGATCGCAGGAGATAAAG

Downstream 100 bases:

>100_bases
TGAATCTTTCAGCCAATGCACAAGCGACCCTCCTGCTAACCAGCGATTTTTCTCGCGCGGCGGCGAGTGAGTATAAACCT
CTTAGTAATAGTGAATGGGG

Product: ATP-dependent DNA helicase RecQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 698

Protein sequence:

>698_residues
MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQRTGWGKSAVYFIASKIFRDRGAGTTIIISPLLALMRN
QVAAAERLGITAETLNSTNREEWQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSK
SGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIH
YQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQRTNLRYGQ
IEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPSAERCGKC
SSCLQHPLLSPDIDSGLLHAANLFIKHADLPLNLNKQMAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEK
KTGRLSDELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQC
QNLDGAFVIIPPLMPGPALLVDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN

Sequences:

>Translated_698_residues
MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQRTGWGKSAVYFIASKIFRDRGAGTTIIISPLLALMRN
QVAAAERLGITAETLNSTNREEWQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSK
SGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIH
YQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQRTNLRYGQ
IEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPSAERCGKC
SSCLQHPLLSPDIDSGLLHAANLFIKHADLPLNLNKQMAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEK
KTGRLSDELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQC
QNLDGAFVIIPPLMPGPALLVDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN
>Mature_698_residues
MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQRTGWGKSAVYFIASKIFRDRGAGTTIIISPLLALMRN
QVAAAERLGITAETLNSTNREEWQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSK
SGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIH
YQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHQFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQRTNLRYGQ
IEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPSAERCGKC
SSCLQHPLLSPDIDSGLLHAANLFIKHADLPLNLNKQMAAGAFTQYGFKGNLPAGLQGSTGRILSRWGDSGWGKQVAQEK
KTGRLSDELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQC
QNLDGAFVIIPPLMPGPALLVDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN

Specific function: Probable DNA helicase. Required for DNA repair and intramolecular recombination; probably has overlapping function with recS (AC P50729). It probably acts to help generate ss-DNA from ds-DNA breaks [H]

COG id: COG0514

COG function: function code L; Superfamily II DNA helicase

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Localized throughout the nucleoid in the presence or absence of DNA double- strand breaks [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HRDC domain [H]

Homologues:

Organism=Homo sapiens, GI4557365, Length=330, Percent_Identity=35.4545454545455, Blast_Score=184, Evalue=3e-46,
Organism=Homo sapiens, GI14591902, Length=349, Percent_Identity=34.3839541547278, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI14591904, Length=349, Percent_Identity=34.3839541547278, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI148612816, Length=331, Percent_Identity=32.9305135951662, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI51242943, Length=332, Percent_Identity=33.7349397590361, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI51242947, Length=332, Percent_Identity=33.7349397590361, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI284005309, Length=340, Percent_Identity=31.1764705882353, Blast_Score=142, Evalue=9e-34,
Organism=Homo sapiens, GI169218196, Length=329, Percent_Identity=30.3951367781155, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI110735439, Length=329, Percent_Identity=30.3951367781155, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI148613856, Length=360, Percent_Identity=23.0555555555556, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI38201710, Length=360, Percent_Identity=23.0555555555556, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI48994977, Length=341, Percent_Identity=37.8299120234604, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787016, Length=353, Percent_Identity=26.9121813031161, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25145561, Length=332, Percent_Identity=36.7469879518072, Blast_Score=180, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI71987993, Length=338, Percent_Identity=34.3195266272189, Blast_Score=175, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71987997, Length=341, Percent_Identity=34.6041055718475, Blast_Score=175, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI32564293, Length=327, Percent_Identity=32.7217125382263, Blast_Score=167, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17552866, Length=342, Percent_Identity=35.3801169590643, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI32565321, Length=369, Percent_Identity=24.1192411924119, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323844, Length=325, Percent_Identity=33.5384615384615, Blast_Score=179, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6324217, Length=224, Percent_Identity=26.3392857142857, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646066, Length=354, Percent_Identity=34.180790960452, Blast_Score=175, Evalue=8e-44,
Organism=Drosophila melanogaster, GI24664230, Length=373, Percent_Identity=32.171581769437, Blast_Score=149, Evalue=7e-36,
Organism=Drosophila melanogaster, GI62472181, Length=373, Percent_Identity=32.171581769437, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI24664226, Length=373, Percent_Identity=32.171581769437, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21358123, Length=359, Percent_Identity=32.5905292479109, Blast_Score=147, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004589
- InterPro:   IPR006293
- InterPro:   IPR002121
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR010997
- InterPro:   IPR018982 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF00570 HRDC; PF09382 RQC [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77479; Mature: 77479

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00690 DEAH_ATP_HELICASE ; PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQRTGWGKSAVYFIASKIF
CCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
RDRGAGTTIIISPLLALMRNQVAAAERLGITAETLNSTNREEWQRISDKLLQGEVDCLLV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
SPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDFRPDYRRILDILRQLPANTPV
CHHHHCCHHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
LGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSK
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCC
SGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVL
CCEEEEEECCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHH
VATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHQF
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHH
FRESAFPAEAQIHEILNVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTE
HHHCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHCCCHHHHHHHHHHHHCCCCCCEEEHH
KLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPSAERCGKC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSCLQHPLLSPDIDSGLLHAANLFIKHADLPLNLNKQMAAGAFTQYGFKGNLPAGLQGST
HHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GRILSRWGDSGWGKQVAQEKKTGRLSDELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLD
CHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHC
LVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQCQNLDGAFVIIPPLMPGPALL
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
VDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCC
>Mature Secondary Structure
MEKHGAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQRTGWGKSAVYFIASKIF
CCCHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
RDRGAGTTIIISPLLALMRNQVAAAERLGITAETLNSTNREEWQRISDKLLQGEVDCLLV
HHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCCCCCEEEE
SPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDFRPDYRRILDILRQLPANTPV
CHHHHCCHHHHHHHHHHHHHHCCEEEEECHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCC
LGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSK
EEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHCC
SGIVYTLTTRDAELVAEWLRKNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVL
CCEEEEEECCCHHHHHHHHHHCCCEEEEEECCEEECCCCCCHHHHHHHHHHHHHHHHHHH
VATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHQF
HHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHH
FRESAFPAEAQIHEILNVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTE
HHHCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHCCCHHHHHHHHHHHHCCCCCCEEEHH
KLWRRTIVSFSPDHERINHLMNQRKSELADVESYITTKECKMQFLRRALDEPSAERCGKC
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SSCLQHPLLSPDIDSGLLHAANLFIKHADLPLNLNKQMAAGAFTQYGFKGNLPAGLQGST
HHHHHCCCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
GRILSRWGDSGWGKQVAQEKKTGRLSDELVEACAEMVCQRWNPHPEPTWVCCVPSLRHLD
CHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHC
LVPDFARRLAAKLGLPFIDAIEKVVDNPPQKMQQNRFHQCQNLDGAFVIIPPLMPGPALL
CCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHH
VDDIVDSAWTLTVLTALLRQAGCPTVYPLALASTSVKN
HHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 9642195 [H]