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The map label for this gene is gspE [H]

Identifier: 170683342

GI number: 170683342

Start: 3316624

End: 3318117

Strand: Reverse

Name: gspE [H]

Synonym: EcSMS35_3246

Alternate gene names: 170683342

Gene position: 3318117-3316624 (Counterclockwise)

Preceding gene: 170681179

Following gene: 170681469

Centisome position: 65.47

GC content: 58.3

Gene sequence:

>1494_bases
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACCCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCAACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Upstream 100 bases:

>100_bases
TAAGCCTGATGCCGCACACCGCGCAGCCGATATTGCCAGCGCAAAATCAGGCCTTACCGCCGGAAGTACGCGCGTTCCTC
AATGCCGGGAGAACGCGTTA

Downstream 100 bases:

>100_bases
TGGCACTGTTTTACTATCAGGCGCTGGAGCGTAATGGTCGCAAAACCAAAGGCATGATTGAGGCGGATTCCGCGCGTCAT
GCCCGTCAATTGTTACGCGG

Product: general secretory pathway protein GspE

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TNEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Sequences:

>Translated_497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TNEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA
>Mature_497_residues
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TNEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=479, Percent_Identity=58.2463465553236, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=45.5729166666667, Blast_Score=313, Evalue=1e-86,
Organism=Escherichia coli, GI87082188, Length=253, Percent_Identity=39.1304347826087, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 55145; Mature: 55145

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLR
CCCCCCCCCCCEEECCCHHCCHHHHEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEHH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEHHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPATNEEKRLLGITDAHAVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEECCCEEEEEC
PQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LAGETSLDEVLRVTMEA
HCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLR
CCCCCCCCCCCEEECCCHHCCHHHHEEECCCCEEEEEECCCHHHHHHHHHHHCCCCEEHH
QIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKL
HCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCHHHHH
INAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLRPGRKLASLLVSRIKVMARLD
HHHHHHHHHHCCCCCCEEEEECCEEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHHHH
IAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
HHHHCCCCCCCEEEEECCEEEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCHHHH
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQ
HHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHCCCCCH
VNTRVGMTFARGLRAILRQDPDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGA
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEECCCEEEEEHHHCCHHHHH
ITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPATNEEKRLLGITDAHAVTLYH
HHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEEEECCCEEEEEC
PQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHH
LAGETSLDEVLRVTMEA
HCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]