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The map label for this gene is gspF [H]

Identifier: 170681469

GI number: 170681469

Start: 3315401

End: 3316624

Strand: Reverse

Name: gspF [H]

Synonym: EcSMS35_3245

Alternate gene names: 170681469

Gene position: 3316624-3315401 (Counterclockwise)

Preceding gene: 170683342

Following gene: 170681001

Centisome position: 65.44

GC content: 59.15

Gene sequence:

>1224_bases
ATGGCACTGTTTTACTATCAGGCGCTGGAGCGTAATGGTCGCAAAACCAAAGGCATGATTGAGGCGGATTCCGCGCGTCA
TGCCCGTCAATTGTTACGCGGTAAAGACCTCATTCCCGTGCACATTGAAGCCCGGATGAATGCATCGGCAGGGGGAATGT
TGCAGCGTCGGCGGCACGCACATCGTCGCGTGGCGGCGGCAGATCTGGCGCTGTTCACTCGTCAACTGGCAACGCTGGTG
CAGGCAGCAATGCCGCTGGAAACCTGCTTACAGGCGGTCAGTGAGCAAAGCGAAAAACTGCATGTAAAAAGCCTCGGAAT
GGCGCTGCGCAGCCGGATTCAGGAAGGTTATACCCTGTCGGACAGCCTGCGCGAACATCCCCGCGTCTTTGACTCCCTGT
TTTGTTCGATGGTCGCCGCCGGAGAAAAATCCGGGCATCTCGACGTGGTGCTCAATCGCCTGGCGGATTACACCGAACAG
CGGCAGCGTCTGAAATCACGCCTGCTGCAGGCCATGCTCTATCCGCTGGTTCTGCTGGTGGTGGCAACGGGCGTAGTCAC
TATTTTGCTGACGGCAGTGGTGCCGAAAATTATCGAACAGTTTGATCATCTCGGACACGCGCTACCCGCCTCCACCCGAA
TGCTCATCGCTATGAGCGACGCGTTACAGGCCAGCGGCGTGTACTGGCTGGCGGGTTTGCTGGGGCTTCTGGTGCTGGGG
CAACGGTTACTCAAAAATCCTGCGATGCGCCTGCGCTGGGATAAAACCTTGCTGCGCCTGCCCGTGACGGGGCGTGTTGC
GCGCGGACTGAATACGGCGCGTTTTTCCCGCACGTTAAGCATCCTCACCGCCAGCAGTGTTCCGCTGCTGGAAGGCATTC
AGACCGCCGCCGCCGTGTCGGCAAATCGTTATGTCGAGCAACAACTGCTGCTGGCGGCAGATCGCGTCCGCGAAGGAAGC
AGCCTGCGCGCCGCGCTGGCGGATCTGCGCCTGTTCCCGCCGATGATGCTGTACATGATCGCCTCCGGCGAACAGAGCGG
CGAGCTGGAAACCATGCTTGAACAGGCCGCGATCAACCAGGAACGGGAATTTGATACCCAGGTGGGTCTGGCGTTAGGGC
TGTTTGAGCCGGCGCTGGTGGTGGTGATGGCGGGCGTGGTGCTGTTTATCGTCATCGCCATCCTCGAACCGATGCTGCAA
CTGAACAATATGGTTGGAATGTAA

Upstream 100 bases:

>100_bases
TCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTGCTCGCCGGAGAAACCTCTCTCGATGAAGTTCTG
CGGGTAACCATGGAGGCGTA

Downstream 100 bases:

>100_bases
TTTACGGAGTTATCACATGAATTCGTTATCCCGCACACAAAAACCACGGGCAGGTTTTACCCTGCTGGAAGTGATGGTGG
TGATTGTTATTCTTGGCGTC

Product: general secretion pathway protein GspF

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAMSDALQASGVYWLAGLLGLLVLG
QRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALADLRLFPPMMLYMIASGEQSGELETMLEQAAINQEREFDTQVGLALGLFEPALVVVMAGVVLFIVIAILEPMLQ
LNNMVGM

Sequences:

>Translated_407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAMSDALQASGVYWLAGLLGLLVLG
QRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALADLRLFPPMMLYMIASGEQSGELETMLEQAAINQEREFDTQVGLALGLFEPALVVVMAGVVLFIVIAILEPMLQ
LNNMVGM
>Mature_406_residues
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQ
AAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQR
QRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAMSDALQASGVYWLAGLLGLLVLGQ
RLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSS
LRAALADLRLFPPMMLYMIASGEQSGELETMLEQAAINQEREFDTQVGLALGLFEPALVVVMAGVVLFIVIAILEPMLQL
NNMVGM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=403, Percent_Identity=45.409429280397, Blast_Score=331, Evalue=6e-92,
Organism=Escherichia coli, GI1786295, Length=409, Percent_Identity=25.6723716381418, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 44863; Mature: 44731

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEARMNASAGGMLQRRRHA
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAMSDALQASGVYWLAGLLGLLVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCHHHEEHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALADLRLFPPMMLYMIASGEQSGELETMLEQAAINQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVVMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKDLIPVHIEARMNASAGGMLQRRRHA
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHH
HRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVLLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPASTRMLIAMSDALQASGVYWLAGLLGLLVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEHHHHHHCCHHHHHHHHHHHHHH
QRLLKNPAMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCHHHEEHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALADLRLFPPMMLYMIASGEQSGELETMLEQAAINQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVVMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]