Click here to switch to the map view.

The map label for this gene is selU [H]

Identifier: 170683025

GI number: 170683025

Start: 553320

End: 554414

Strand: Reverse

Name: selU [H]

Synonym: EcSMS35_0546

Alternate gene names: 170683025

Gene position: 554414-553320 (Counterclockwise)

Preceding gene: 170682910

Following gene: 170679602

Centisome position: 10.94

GC content: 55.53

Gene sequence:

>1095_bases
ATGCAAGAGAGACACACGGAACAGGACTATCGTGCCCTGCTGATTGCTGATACGCCCATTATTGATGTTCGCGCCCCCAT
CGAGTTTGAGCAAGGCGCAATGCCTGCCGCTATCAACCTGCCGTTAATGAATAACGATGAACGCGCCGCCGTTGGCACCT
GCTATAAACAGCAAGGGTCAGACGCGGCGCTGGCGCTGGGTCATAAACTGGTGGCGGGTGAAATTCGTCAGCAGCGCATG
GACGCCTGGCGGGCAGCGTGCCTGCAAAATCCGCAAGGTATTCTCTGCTGCGCCCGAGGCGGTCAGCGCTCACATATTGT
CCAAAGCTGGTTGCATGCAGCGGGGATTGATTATCCGCTGGTGGAAGGCGGTTATAAGGCACTGCGCCAGACCGCGATTC
AGGCGACTATTGAACTGGCACAAAAACCGATAGTGCTGATTGGCGGTTGTACCGGCAGCGGTAAAACGCTGTTAGTGCAG
CAACAGCCGAACGGTGTTGATCTGGAAGGGTTGGCGCGTCATCGCGGTTCGGCGTTTGGTCGCACCTTACAACCACAACT
TAGCCAGGCGAGTTTTGAAAACCTGCTGGCTGCCGAAATGCTAAAAACCGACGCCCGTCAGAATTTGCGCCTGTGGGTGC
TGGAAGACGAAAGCCGGATGATCGGTTCGAATCACCTGCCGGAATGCCTGCGCGAGCGAATGACTCAGGCGGCGATTGCG
GTGGTAGAAGATCCGTTTGAGATCCGTCTTGAGCGCCTGAACGAAGAGTATTTCTTGCGTATGCATCATGATTTTACCCA
CGCGTACGGCGACGAACAGGGCTGGCAGGAGTATTGCGAATACCTGCATCACGGACTTTCGGCTATTAAGCGTCGGCTGG
GGCTACAGCGATATAACGAACTGGCTGCAAGGCTGGATGCAGCACTGACAACGCAACTCACCACCGGCAGCACCGACGGT
CATCTGGCCTGGCTGGTGCCGTTACTTGAAGAATATTACGACCCGATGTATCGCTATCAGCTTGAGAAAAAAGCGGAAAA
AGTTGTCTTTCGCGGTGAGTGGGCGGAAGTGGCGGAATGGGTGAAGTCGCGGTAA

Upstream 100 bases:

>100_bases
TTTTAGAAATTTTCGGCAACCCACGCAGTTAAAAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGA
TTTGAGGTTCACTTTTTACT

Downstream 100 bases:

>100_bases
TAGGCGGTATTCTGTGCACGCTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGTTAAGTGCAGTTTTGT
AGGCCTGATAAGACGCGCCA

Product: tRNA 2-selenouridine synthase

Products: NA

Alternate protein names: Selenophosphate-dependent tRNA 2-selenouridine synthase [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKSR

Sequences:

>Translated_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKSR
>Mature_364_residues
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRM
DAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDG
HLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEWVKSR

Specific function: Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA [H]

COG id: COG2603

COG function: function code R; Predicted ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1786712, Length=364, Percent_Identity=99.4505494505495, Blast_Score=748, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR017582 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: NA

Molecular weight: Translated: 41118; Mature: 41118

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHCCC
DAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKSR
HHCC
>Mature Secondary Structure
MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMNNDERAAVGTCYKQQGS
CCCCCCCCHHEEEEEECCCEEEEECCCCCCCCCCCCEEECCEECCCCCHHHHHHHHHCCC
DAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCH
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCHHHHHHHCCHHCC
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHH
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
HHCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
HHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHEEECCCHHHHHHH
VKSR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA