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The map label for this gene is allS [H]

Identifier: 170682910

GI number: 170682910

Start: 554483

End: 555409

Strand: Reverse

Name: allS [H]

Synonym: EcSMS35_0547

Alternate gene names: 170682910

Gene position: 555409-554483 (Counterclockwise)

Preceding gene: 170682104

Following gene: 170683025

Centisome position: 10.96

GC content: 51.78

Gene sequence:

>927_bases
ATGTTCGATCCAGAAACCTTGCGGACTTTCATTGCGGTTGCTGAAACAGGTAGTTTTTCAAAAGCGGCAGAACGATTATG
TAAAACCACGGCGACGATCAGTTATCGCATTAAACTTCTGGAAGAGAATACCGGAGTAGCGCTGTTTTTCCGTACGACTC
GTAGCGTAACGTTGACAGCGGCTGGCGAGCATCTACTTTCCCAGGCCAGAGACTGGCTGAGCTGGCTGGAAAGTATGCCA
AGCGAGCTGCAACAGGTGAATGATGGCGTGGAACGCCAGGTGAATATTGTCATCAACAACCTGCTCTATAACCCCCAGGC
CGTTGCCCAGTTGCTGGCGTGGCTGAATGAGCGTTACCCCTTTACCCAGTTTCACATCTCCCGACAAATCTATATGGGCG
TCTGGGACTCGCTATTGTACGAAGGTTTTTCGCTGGCTATCGGCGTCACGGGAACTGAGGCGCTGGCAAATACCTTTAGT
CTTGATCCCTTAGGATCGGTGCAATGGCGCTTTGTCATGGCGGCGGATCATCCGCTGGCGAACGTTGAAGAGCCGCTAAC
AGAAGCGCAGTTGCGGCGCTTTCCGGCGGTCAATATTGAAGACAGCGCCCGCACACTAACCAAACGCGTCGCCTGGCGAT
TGCCGGGGCAAAAAGAGATTATTGTTCCCGATATGGAAACGAAAATCGCCGCCCATCTGGCGGGCGTTGGCATTGGTTTT
TTGCCAAAATCACTTTGCCAGTCAATGATCGATAATCAACAACTGGTCAGCCGGGTAATCCCAACGATGCGCCCTCCTTC
ACCATTGAGTCTGGCATGGCGCAAATTTGGCAGCGGCAAAGCGATAGAAGATATTGTGACCTTGTTTACCCAGCGCAGGC
CGGAAATCAGCGGATTTTTAGAAATTTTCGGCAACCCACGCAGTTAA

Upstream 100 bases:

>100_bases
TTTAGTCCACAAATTGAACAGGGTTATCATGTAAAAATACTGATAAATCAGAGGATAAGAAAATCACGCAACTTGCGGAT
TTAAAAGGGGATACCTGAAG

Downstream 100 bases:

>100_bases
AAATCAAGTTTCCTTGCGCGTTGGCGCGTAAAATAGCGCGCAAGCTGATTTGAGGTTCACTTTTTACTATGCAAGAGAGA
CACACGGAACAGGACTATCG

Product: DNA-binding transcriptional activator AllS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS

Sequences:

>Translated_308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS
>Mature_308_residues
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMP
SELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAIEDIVTLFTQRRPEISGFLEIFGNPRS

Specific function: Positive regulator essential for the expression of AllD operon. Binds to the AllD promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786713, Length=308, Percent_Identity=99.6753246753247, Blast_Score=632, Evalue=0.0,
Organism=Escherichia coli, GI1786508, Length=283, Percent_Identity=38.5159010600707, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787949, Length=275, Percent_Identity=30.9090909090909, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI87082024, Length=277, Percent_Identity=24.9097472924188, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1789492, Length=281, Percent_Identity=26.6903914590747, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=30.1204819277108, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787879, Length=258, Percent_Identity=27.906976744186, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=120, Percent_Identity=32.5, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=175, Percent_Identity=29.1428571428571, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34526; Mature: 34526

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAIEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC
>Mature Secondary Structure
MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENTGVALFFRTTRSVTLTA
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEH
AGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLLYNPQAVAQLLAWLNERYP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
FTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFSLDPLGSVQWRFVMAADHPLA
CHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHCCCCCCCCCEEEEEEECCCCCC
NVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQKEIIVPDMETKIAAHLAGVGIGF
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHCCHHH
LPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKFGSGKAIEDIVTLFTQRRPEISGFL
HHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH
EIFGNPRS
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]