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The map label for this gene is plsB [H]

Identifier: 170682968

GI number: 170682968

Start: 4601592

End: 4604075

Strand: Reverse

Name: plsB [H]

Synonym: EcSMS35_4503

Alternate gene names: 170682968

Gene position: 4604075-4601592 (Counterclockwise)

Preceding gene: 170683816

Following gene: 170679620

Centisome position: 90.84

GC content: 54.95

Gene sequence:

>2484_bases
ATGACTTTCTGCTATCCTTGCCGCGCATTTGCATTATTAACCAGAGGCTTTACATCGTTTATGTCCGGCTGGCCACGAAT
TTACTACAAATTACTGAATTTACCATTAAGCATCCTGGTAAAAAGCAAGTCTATTCCGGCAGATCCTGCCCCGGAACTGG
GGCTGGATACCTCTCGTCCAATTATGTACGTTTTACCGTACAACTCGAAAGCTGACTTGCTGACGTTGCGCGCCCAGTGT
CTGGCACATGACCTGCCTGACCCGTTAGAGCCGCTGGAAATCGACGGCACGCTACTGCCGCGCTATGTGTTCATTCACGG
CGGGCCGCGTGTGTTCACCTATTACACGCCGAAAGAAGAGTCTATTAAGCTGTTCCACGACTATCTCGATTTGCACCGTA
GCAACCCAAATCTGGATGTGCAGATGGTGCCAGTATCGGTGATGTTTGGTCGCGCGCCGGGGCGTGAAAAAGGCGAAGTG
AACCCGCCGCTGCGTATGCTTAACGGCGTACAGAAATTTTTCGCTGTACTGTGGCTCGGTCGCGACAGTTTTGTGCGTTT
CTCGCCGTCAGTTTCGCTGCGCCGTATGGCGGATGAACACGGCACGGATAAAACTATCGCGCAGAAACTGGCGCGCGTGG
CGCGTATGCACTTTGCCCGTCAACGTCTGGCTGCCGTAGGCCCACGTCTGCCTGCTCGTCAGGATCTGTTTAATAAGCTG
CTCGCCTCCCGCGCCATTGCCAAAGCGGTAGAAGATGAAGCGCGCAGCAAAAAAATCTCCCATGAAAAAGCGCAGCAGAA
CGCGATTGTGCTGATGGAAGAGATTGCGGCGAATTTCTCTTACGAGATGATCCGCCTGACTGACCGCATTCTGGGCTTCA
CCTGGAACCGCCTTTACCAGGGTATCAACGTCCATAACGCCGAGCGCGTTCGCCAGTTGGCCCACGACGGTCATGAGCTG
GTGTATGTGCCTTGCCACCGCAGTCACATGGACTACCTGCTGCTTTCTTACGTGCTGTATCACCAGGGGCTGGTGCCGCC
GCATATCGCCGCCGGGATCAACCTGAACTTCTGGCCGGCCGGGCCGATTTTCCGCCGTCTGGGTGCGTTCTTTATTCGCC
GTACGTTTAAAGGCAATAAGCTCTATTCCACCGTTTTCCGTGAGTATCTCGGCGAACTGTTCAGCCGTGGTTATTCCGTC
GAGTATTTCGTGGAAGGCGGTCGTTCCCGTACGGGGCGTTTGCTGGATCCGAAAACCGGTACGCTGTCGATGACCATTCA
GGCGATGCTGCGTGGCGGCACGCGTCCGATTACGCTGATTCCGATCTATATCGGTTATGAGCACGTCATGGAAGTGGGTA
CTTACGCCAAAGAACTGCGCGGTGCAACGAAAGAGAAAGAGAGCCTGCCGCAGATGCTGCGCGGTTTAAGCAAGCTGCGT
AATCTCGGTCAGGGTTACGTCAACTTCGGTGAACCAATGCCGTTGATGACCTACCTTAACCAGCACGTACCAGACTGGCG
TGAATCTATCGATCCCATCGAAGCGGTGCGTCCGGCCTGGTTAACGCCGACGGTCAATAATATTGCTGCCGATCTGATGG
TACGCATTAACAACGCAGGCGCGGCAAACGCCATGAACCTGTGCTGTACTGCGCTACTGGCGTCACGTCAGCGCTCACTC
ACCCGCGAGCAGTTAACCGAGCAACTCAACTGCTACCTGGATCTGATGCGCAACGTACCTTACTCCACGGACTCTACCGT
TCCTTCAGCCAGCGCCAGCGAGCTTATCGATCACGCGCTGCAAATGAACAAGTTTGAAGTCGAGAAAGACACTATCGGCG
ACATCATCATTCTGCCGCGCGAGCAAGCGGTGTTGATGACCTACTATCGCAACAACATTGCGCATATGTTGGTGCTGCCT
TCGCTGATGGCGGCAATTGTCACTCAGCATCGCCACATCTCCCGCGACGTATTGATGGAGCACGTCAATGTGCTTTACCC
AATGCTGAAAGCGGAGCTGTTCCTGCGCTGGGATCGCGACGAATTGCCAGACGTTATTGATGCGCTGGCAAATGAGATGC
AACGTCAGGGGCTGATTACCCTGCAAGATGATGAGTTGCATATCAACCCGGCGCATTCTCGCACGCTACAGCTGCTGGCC
GCAGGCGCGCGCGAAACGCTGCAACGTTATGCCATTACCTTCTGGCTGTTGAGCGCCAACCCGTCGATCAACCGCGGTAC
GCTGGAAAAAGAGAGCCGCACCGTCGCGCAACGTCTCTCCGTGCTGCACGGTATCAACGCGCCGGAGTTCTTCGACAAAG
CGGTGTTCAGTTCGCTGGTGCTGACGCTGCGTGATGAAGGGTATATCAGCGATAGCGGCGATGCCGAACCGGCAGAAACG
ATGAAGGTTTATCAGTTGCTGGCGGAGTTGATTACATCAGACGTGCGTTTGACGATTGAGAGTGCGACGCAGGGCGAGGG
GTAA

Upstream 100 bases:

>100_bases
TTTTTACATTATGAGCGTCAATATCAGTGTACCGTTAAATCCGATGATTCGGGTTTGCGTAACGCCGCGCGAAACATGAG
CGGATACCACAGAATTTCCC

Downstream 100 bases:

>100_bases
TCAGTGAGAATTGCCGGATGCGGCTCAAACGCCTTATCCGGCCTTCCATGATCTGCAAATTCAATAAATTGCGATCTGTT
GTGTAGGCCTGAGAAGCGCA

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT [H]

Number of amino acids: Translated: 827; Mature: 826

Protein sequence:

>827_residues
MTFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQC
LAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEV
NPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKL
LASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHEL
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSV
EYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLR
NLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSL
TREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLP
SLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLA
AGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAET
MKVYQLLAELITSDVRLTIESATQGEG

Sequences:

>Translated_827_residues
MTFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQC
LAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEV
NPPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKL
LASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHEL
VYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSV
EYFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLR
NLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSL
TREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLP
SLMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLA
AGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAET
MKVYQLLAELITSDVRLTIESATQGEG
>Mature_826_residues
TFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYVLPYNSKADLLTLRAQCL
AHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVN
PPLRMLNGVQKFFAVLWLGRDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKLL
ASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWNRLYQGINVHNAERVRQLAHDGHELV
YVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVE
YFVEGGRSRTGRLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLRN
LGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAGAANAMNLCCTALLASRQRSLT
REQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPS
LMAAIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLAA
GARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETM
KVYQLLAELITSDVRLTIESATQGEG

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family [H]

Homologues:

Organism=Homo sapiens, GI7657134, Length=418, Percent_Identity=29.4258373205742, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI190358539, Length=272, Percent_Identity=33.4558823529412, Blast_Score=134, Evalue=5e-31,
Organism=Escherichia coli, GI87082362, Length=807, Percent_Identity=99.8760842627014, Blast_Score=1664, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71988723, Length=278, Percent_Identity=31.6546762589928, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25147672, Length=279, Percent_Identity=30.1075268817204, Blast_Score=139, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI71988728, Length=190, Percent_Identity=37.8947368421053, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21357731, Length=499, Percent_Identity=25.4509018036072, Blast_Score=143, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24650752, Length=499, Percent_Identity=25.4509018036072, Blast_Score=143, Evalue=6e-34,
Organism=Drosophila melanogaster, GI24650754, Length=499, Percent_Identity=25.4509018036072, Blast_Score=143, Evalue=6e-34,
Organism=Drosophila melanogaster, GI17864692, Length=270, Percent_Identity=30.3703703703704, Blast_Score=142, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002123
- InterPro:   IPR022284 [H]

Pfam domain/function: PF01553 Acyltransferase [H]

EC number: =2.3.1.15 [H]

Molecular weight: Translated: 93726; Mature: 93594

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRP
CCEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCC
IMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEE
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEEEEECCCEEEEEECCCHH
SIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKL
CCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWNRLYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPA
CCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEECCCC
GPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTG
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCC
TLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLR
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAG
HCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEECCCC
AANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLME
HCCCEEECCCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLA
HHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHH
AGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLV
HHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TFCYPCRAFALLTRGFTSFMSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRP
CEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCC
IMYVLPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEE
EEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECEEEEEECCCEEEEEECCCHH
SIKLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
HHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFNKL
CCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
LASRAIAKAVEDEARSKKISHEKAQQNAIVLMEEIAANFSYEMIRLTDRILGFTWNRLYQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
GINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGINLNFWPA
CCCCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEECCCC
GPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTGRLLDPKTG
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEECCCCC
TLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLPQMLRGLSKLR
HHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
NLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNIAADLMVRINNAG
HCCCCCCCCCCCCHHHHHHHHCCCCHHHCCCHHHHCCCCCCCCCHHHHHHHHHEEECCCC
AANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDSTVPSASASELIDHAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
QMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMAAIVTQHRHISRDVLME
HCCCEEECCCCCCCEEEECCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQDDELHINPAHSRTLQLLA
HHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCCHHHHHHHHH
AGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLSVLHGINAPEFFDKAVFSSLV
HHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLTIESATQGEG
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA