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The map label for this gene is dcuB [H]

Identifier: 170681956

GI number: 170681956

Start: 1140962

End: 1142278

Strand: Reverse

Name: dcuB [H]

Synonym: EcSMS35_1122

Alternate gene names: 170681956

Gene position: 1142278-1140962 (Counterclockwise)

Preceding gene: 170683952

Following gene: 170683932

Centisome position: 22.54

GC content: 44.04

Gene sequence:

>1317_bases
ATGGACGCTCTATTTTTTATACAACTATTTATTGTTCTGGCCTGTATTGGAATCGGCGGAAAATACGGCGGAATGGGGCT
TGGTGCTGGAGGTGGGTTAGGGGTTGCAATATTGGTTTTGGGCTTTGGACTGAAACCCTCCTCTCCCTCCATTACTGCTA
TGTTAATTATTATTTGTGTTATTAGTGCTGTCAGTATATTACAAGCTGTTGGAGGATTAGATTATTTAGTCAGGGTCGCA
GAACGGATACTCCGTAAGAAACCACAGGCGATAACCTTTGTAGCGCCAATATTAACTTCAGTATTTACACTATTTTGTGG
GACGACTTATGTCGCTTTCTCCTTATACCCTGTTATTGCTGAAGTCGCCGCTGAAGCAAAAGTACGTCCAGAACGTGCAT
TATCTGCAACGGTCATTGCAGCCAGTGTGGCAGTGGCTGCCAGCCCTATGAGTGCAGCAACTGCCGGGATGCTGGCTATA
CTTCATGAGTATGCAGGGATCACGTTAGGGCAAATATTATCTATTGCCCTTCCTTCGTTTTTTATGGCTGCAATTGTTAC
CAGCTTTTCAGTCTACAAGCGTGGTAAAGAACTGGAAGATGATCCTGAATTTCAAAGGCGTGTTGCAGCAGGTGAGTATG
AGTTCATGCACACCGAACAGAAAAAAGAATATGTCGCTGCACCAGGTGCAAGAAAAGGGGTTGCTATTTTTGCCATAGGA
GTCGTGCTGGTCCTTATTCTCGGATCTTTTACTGAGCTTCTTCCGTCGTGGGATGGAAAAAGATTATCAACCCCAATGGT
CATCCAGATGATTATGTTGACTGCAGCTTTATTGATCATGATTGTGGGGAAAGTTCCAAGCAATAAATTTAACAGTGGCT
CTGTGTTCCGTGCTGGTTTGATGGGGGTTGTGGCAATACTTGGTGTTTCGTGGATGACTGCAACATTTTTTGATGCTTAT
CAGGCAGAGCTGATCAATGTTTTTGGCAATCTTGTAAATGATGCGCCTTTGCTGTTTGGCTTTATCGTATTCTTGTTTTC
TCTGGTGATCATGAGCCCGGCTGCGACAGTAGCTGCGATTATGCCATTAGGAGTCACCCTGGGTATTCCTGCACCTTATC
TTATTGCAATTTTTGCCTGTACTTGTGGTGATTTTATTATACCCGGAGCGAATCAGATTGGTTGTGTGGCGTTTGACAGA
ACTGGTACTACCAGAATTGGGCGCTTTGTTATCAACCATAGCTATATACGTCCTGGGTTCGTCATGGTCATTTCCCAGGT
TGTTTTTGCCTACTTAATTGCGCAGGTTATTCTGTAA

Upstream 100 bases:

>100_bases
AAACAAATAGAGATTGCAAAATCACTTTTAAAATAGTTAAGAATTATAATGTTTAAGATTCCCCGGATATTATATCCGGG
AATATCTTTGAGGTTGAAAA

Downstream 100 bases:

>100_bases
CATTAAGGAATAAATGAAGAGGATAACACTGTGGCATGACGAATTAGCCCCCTTCAGCGTTTTTGAGGAAGGGGGATTAA
GAAAATATTACACAGCTGTA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGLKPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVA
ERILRKKPQAITFVAPILTSVFTLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHTEQKKEYVAAPGARKGVAIFAIG
VVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAALLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAY
QAELINVFGNLVNDAPLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQIGCVAFDR
TGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL

Sequences:

>Translated_438_residues
MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGLKPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVA
ERILRKKPQAITFVAPILTSVFTLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHTEQKKEYVAAPGARKGVAIFAIG
VVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAALLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAY
QAELINVFGNLVNDAPLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQIGCVAFDR
TGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL
>Mature_438_residues
MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGLKPSSPSITAMLIIICVISAVSILQAVGGLDYLVRVA
ERILRKKPQAITFVAPILTSVFTLFCGTTYVAFSLYPVIAEVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAI
LHEYAGITLGQILSIALPSFFMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHTEQKKEYVAAPGARKGVAIFAIG
VVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAALLIMIVGKVPSNKFNSGSVFRAGLMGVVAILGVSWMTATFFDAY
QAELINVFGNLVNDAPLLFGFIVFLFSLVIMSPAATVAAIMPLGVTLGIPAPYLIAIFACTCGDFIIPGANQIGCVAFDR
TGTTRIGRFVINHSYIRPGFVMVISQVVFAYLIAQVIL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790565, Length=409, Percent_Identity=35.4523227383863, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1790580, Length=440, Percent_Identity=36.5909090909091, Blast_Score=231, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46350; Mature: 46350

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGLKPSSPSITAMLIIICV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ISAVSILQAVGGLDYLVRVAERILRKKPQAITFVAPILTSVFTLFCGTTYVAFSLYPVIA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAILHEYAGITLGQILSIALPSF
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHTEQKKEYVAAPGARKGVAIFAIG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAALLIMIVGKVPSNKFNSGSVFRAGL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
MGVVAILGVSWMTATFFDAYQAELINVFGNLVNDAPLLFGFIVFLFSLVIMSPAATVAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
MPLGVTLGIPAPYLIAIFACTCGDFIIPGANQIGCVAFDRTGTTRIGRFVINHSYIRPGF
HHHCHHCCCCHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHH
VMVISQVVFAYLIAQVIL
HHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDALFFIQLFIVLACIGIGGKYGGMGLGAGGGLGVAILVLGFGLKPSSPSITAMLIIICV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
ISAVSILQAVGGLDYLVRVAERILRKKPQAITFVAPILTSVFTLFCGTTYVAFSLYPVIA
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVAAEAKVRPERALSATVIAASVAVAASPMSAATAGMLAILHEYAGITLGQILSIALPSF
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
FMAAIVTSFSVYKRGKELEDDPEFQRRVAAGEYEFMHTEQKKEYVAAPGARKGVAIFAIG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VVLVLILGSFTELLPSWDGKRLSTPMVIQMIMLTAALLIMIVGKVPSNKFNSGSVFRAGL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
MGVVAILGVSWMTATFFDAYQAELINVFGNLVNDAPLLFGFIVFLFSLVIMSPAATVAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
MPLGVTLGIPAPYLIAIFACTCGDFIIPGANQIGCVAFDRTGTTRIGRFVINHSYIRPGF
HHHCHHCCCCHHHHHHHHHHHCCCEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCHH
VMVISQVVFAYLIAQVIL
HHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]