Click here to switch to the map view.

The map label for this gene is argH [H]

Identifier: 170683952

GI number: 170683952

Start: 1142343

End: 1143719

Strand: Reverse

Name: argH [H]

Synonym: EcSMS35_1123

Alternate gene names: 170683952

Gene position: 1143719-1142343 (Counterclockwise)

Preceding gene: 170680569

Following gene: 170681956

Centisome position: 22.57

GC content: 40.6

Gene sequence:

>1377_bases
ATGTCTACGAAATTATGGGGCGGACGTTTTGATATGCCAACAAATAAACTCGTTGAACAATATAATGCAACTATCACGCT
GGAGCAGCGCCTATGCCCCTTTGATATTCAAGGAAGCATAGTTCATGCCACGATGTTAGGACGTCAGGGAATAATCACAC
AGGATGAAGCCAATACAATTATTAGAGGTTTGAGACAAGTAAGTAAAGAAATTGAAGACGGCCAATTCATTTTCGATACT
GTAGATGAAGATATTCATATGGCCATTGAAAGACGGATGACTGAAATTATTGGCCCTGTTGGTGGTAAACTCCACACGGG
GCGAAGTCGTAATGATCAAACCACTGTTGATTCTAAAATGCATATGCGAGCAATTATCCGTGAAATTCAAGAGGATATTA
CCAACCTGCAAAAAATAATAATTAACAAAGCAGAAAACAATATTAATGTCATCATGCCAGGCTATACTCATTTGCAAACA
GGTCAGCCCATTCTTTTATCTCACTGGATTATGGCATATTACTGGATGTTGCGTCGTGACTGGAATAGGTTTGAAGATCT
GTATCAACGGATGGGAGAATGCCCTTTGGGGGCAGCGGCTCTCGCCGGTACGACATTCCCTATTGATCGTAATTTTACGG
CTCGTGAACTTGGTTTTGATAAGCCAACTGAGAATAGTATTGATTCTGTCAGTGACCGCGACCATATGGTCGAATTCACC
GCGGCAGCAGCGATGTGTTTTATGCATCTAACTCGCCTTTCAGAGGAACTGATTTTATTCTCTAGCCAAGACTTTAAATT
TATTGAACTTTCTGATGACTTCTGTACAGGATCCAGCATCATGCCGCAGAAAAAGAACCCTGATGTGGCGGAAAAAATGC
GTGGTAAAGGTGGGAGAATGTATGGAAATCTGATGGCCATGCTGACTATTATGAAAGGCATACCGCTAGCGTATAATACA
GACATGAGCGAGGATAAAGAGCAGGTCTATGACTCAATGGATACTCTACAGGCCAGCTTAAGAATAATGGCACCTATGAT
CGAAAAAATGGTTATCCTTGCCGAAAATACGCGTGCAGCAGCCGCTCGAGGATTCTCGAATGCAACAGATATGGCCGATT
ATCTGGTCCGTAAAGGTATTCCTTTCAGAGAAGCTCACCATATTGTTGGTAGTGCAGTAAATTACTGTATTAAACATAAA
AAAATGTTAGAAGAGCTTACTATGGAAGAATTCTCCACATTTGATAATAAAATAGAAAAAGATATTTATGAAAGTATTTC
TCTGGAGGCTTGCATTAAGGCCAGGATGTCTTATGGTGGAACCGGACCTGATGCTGTCAAAAAACAAATAGAGATTGCAA
AATCACTTTTAAAATAG

Upstream 100 bases:

>100_bases
GCCGCATTTGTTGACAATTGCCAACATCATTTTATAACCTGATATTAAATCCAGCCGGTACATTTTTTCTAATTACTTAT
GAATAAGCAGGAGGATGAGG

Downstream 100 bases:

>100_bases
TTAAGAATTATAATGTTTAAGATTCCCCGGATATTATATCCGGGAATATCTTTGAGGTTGAAAAATGGACGCTCTATTTT
TTATACAACTATTTATTGTT

Product: argininosuccinate lyase ArgH-like protein

Products: NA

Alternate protein names: ASAL; Arginosuccinase [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MSTKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQDEANTIIRGLRQVSKEIEDGQFIFDT
VDEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQTTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQT
GQPILLSHWIMAYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDSVSDRDHMVEFT
AAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNPDVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNT
DMSEDKEQVYDSMDTLQASLRIMAPMIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHK
KMLEELTMEEFSTFDNKIEKDIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK

Sequences:

>Translated_458_residues
MSTKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQDEANTIIRGLRQVSKEIEDGQFIFDT
VDEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQTTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQT
GQPILLSHWIMAYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDSVSDRDHMVEFT
AAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNPDVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNT
DMSEDKEQVYDSMDTLQASLRIMAPMIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHK
KMLEELTMEEFSTFDNKIEKDIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK
>Mature_457_residues
STKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQDEANTIIRGLRQVSKEIEDGQFIFDTV
DEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQTTVDSKMHMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQTG
QPILLSHWIMAYYWMLRRDWNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDSVSDRDHMVEFTA
AAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNPDVAEKMRGKGGRMYGNLMAMLTIMKGIPLAYNTD
MSEDKEQVYDSMDTLQASLRIMAPMIEKMVILAENTRAAAARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHKK
MLEELTMEEFSTFDNKIEKDIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK

Specific function: Arginine biosynthesis; eighth (last) step. [C]

COG id: COG0165

COG function: function code E; Argininosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Argininosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31541964, Length=457, Percent_Identity=39.8249452954048, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303542, Length=457, Percent_Identity=39.8249452954048, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI68303549, Length=457, Percent_Identity=38.074398249453, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI68303547, Length=457, Percent_Identity=38.2932166301969, Blast_Score=334, Evalue=1e-91,
Organism=Escherichia coli, GI1790398, Length=454, Percent_Identity=42.7312775330396, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321806, Length=459, Percent_Identity=40.958605664488, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI221473854, Length=451, Percent_Identity=36.3636363636364, Blast_Score=319, Evalue=3e-87,
Organism=Drosophila melanogaster, GI78706858, Length=451, Percent_Identity=36.3636363636364, Blast_Score=319, Evalue=3e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009049
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF00206 Lyase_1 [H]

EC number: =4.3.2.1 [H]

Molecular weight: Translated: 51996; Mature: 51864

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQDEANTI
CCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHH
IRGLRQVSKEIEDGQFIFDTVDEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQTTVDSKM
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
HMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQTGQPILLSHWIMAYYWMLRRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
WNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDSVSDRDHMVEFT
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHH
AAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNPDVAEKMRGKGGRM
HHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCH
YGNLMAMLTIMKGIPLAYNTDMSEDKEQVYDSMDTLQASLRIMAPMIEKMVILAENTRAA
HHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHKKMLEELTMEEFSTFDNKIEK
HHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
STKLWGGRFDMPTNKLVEQYNATITLEQRLCPFDIQGSIVHATMLGRQGIITQDEANTI
CCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCCCCCCEEEHHHHCCCCCCCCHHHHHH
IRGLRQVSKEIEDGQFIFDTVDEDIHMAIERRMTEIIGPVGGKLHTGRSRNDQTTVDSKM
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHH
HMRAIIREIQEDITNLQKIIINKAENNINVIMPGYTHLQTGQPILLSHWIMAYYWMLRRD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHH
WNRFEDLYQRMGECPLGAAALAGTTFPIDRNFTARELGFDKPTENSIDSVSDRDHMVEFT
HHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHH
AAAAMCFMHLTRLSEELILFSSQDFKFIELSDDFCTGSSIMPQKKNPDVAEKMRGKGGRM
HHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCH
YGNLMAMLTIMKGIPLAYNTDMSEDKEQVYDSMDTLQASLRIMAPMIEKMVILAENTRAA
HHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
AARGFSNATDMADYLVRKGIPFREAHHIVGSAVNYCIKHKKMLEELTMEEFSTFDNKIEK
HHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYESISLEACIKARMSYGGTGPDAVKKQIEIAKSLLK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA