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The map label for this gene is dgoD1

Identifier: 170680994

GI number: 170680994

Start: 1110613

End: 1111761

Strand: Reverse

Name: dgoD1

Synonym: EcSMS35_1095

Alternate gene names: 170680994

Gene position: 1111761-1110613 (Counterclockwise)

Preceding gene: 170681749

Following gene: 170680666

Centisome position: 21.94

GC content: 51.96

Gene sequence:

>1149_bases
ATGAAAATCACCAAAATTACCACGTATCGTTTACCTCCCCGCTGGATGTTCCTGAAAATTGAAACCGATGAAGGCGTGGT
CGGCTGGGGCGAGCCCGTGATCGAAGGCCGCGCCCGTACGGTGGAAGCGGCAGTTCACGAGCTGGGTGACTATTTGATTG
GTCAGGATCCATCGCGCATCAATGACTTATGGCAAGTGATGTATCGCGCCGGATTTTATCGTGGAGGTCCAATCCTGATG
AGCGCCATTGCCGGGATCGACCAGGCGTTATGGGATATCAAAGGCAAAGTGCTGAATTCGCCGGTCTGGCAACTGATGGG
CGGCCTGGTGCGTGACAAAATTAAAGCCTACAGTTGGGTCGGCGGCGATCGTCCGGCGAATGTTATCGACGGAATAAAAA
TCCTGCGCGAAATCGGTTTTGATACCTTCAAACTGAATGGTTGTGAAGAACTGGGGCTAATTGATAACTCCCGCGCGGTA
GATGCGGCGGTCAACACCGTGGCACAAATTCGTGAAGCTTTTGGCAATCAGATTGAGTTTGGTCTTGATTTCCACGGTCG
CGTCAGCGCGCCGATGGCGAAAGTACTGATTAAAGAACTGGAACCGTATCGCCCGCTGTTTATTGAAGAGCCGGTGCTGG
CAGAACAGGCCGAATACTACCCGAAACTGGCGGCACAAACGCATATTCCACTGGCGGCGGGTGAGCGCATGTTCTCACGT
TTCGATTTTAAACGTGTGCTGGAGGCGGGTGGTATTTCGATTCTGCAACCGGATCTCTCCCACGCAGGCGGTATTACCGA
ATGCTACAAAATTGCCGGAATGGCAGAAGCCTATGACGTGACCCTTGCGCCGCACTGTCCGCTCGGACCGATTGCACTGG
CAGCTTGCCTGCATATCGACTTTGTTTCCTATAACGCCGTACTTCAGGAACAAAGTATGGGCATTCATTACAACAAAGGC
GCGGAGTTACTCGACTTTGTGAAAAACAAAGAGGACTTCAGCATGGTCGGCGGCTTCTTTAAACCGCTAACGAAACCGGG
CTTAGGCGTGGAAATCGACGAAGCTAAAGTTATTGAGTTCAGTAAAAATGCCCCGGACTGGCGTAATCCGCTCTGGCGTC
ATGAAGATAACAGCGTAGCAGAGTGGTAA

Upstream 100 bases:

>100_bases
GGGCAGGCTTAGGCAGCGATCTCTATCGCGCCGGGCAGTCCGTAGAACGCACCGCGCAGCAGGCAGCAGCATTTGTTAAG
GCGTATCGAGAGGCAGTGCA

Downstream 100 bases:

>100_bases
TTCCTGCCACGTAAGCCCCTCATCGGGCACTAAAACAGCAATACAAAAATATAACCCTCTGTAAATTACAGGGCATGGTG
AGCGGCTTCGCTATGCCCAA

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD 1

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNSPVWQLMGGLVRDKIKAYSWVGGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW

Sequences:

>Translated_382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNSPVWQLMGGLVRDKIKAYSWVGGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW
>Mature_382_residues
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILM
SAIAGIDQALWDIKGKVLNSPVWQLMGGLVRDKIKAYSWVGGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAV
DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKG
AELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=99.2146596858639, Blast_Score=781, Evalue=0.0,
Organism=Escherichia coli, GI1787864, Length=414, Percent_Identity=30.4347826086957, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI226510960, Length=243, Percent_Identity=30.8641975308642, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DGOD1_ECOSM (B1LPS5)

Other databases:

- EMBL:   CP000970
- RefSeq:   YP_001743171.1
- ProteinModelPortal:   B1LPS5
- SMR:   B1LPS5
- EnsemblBacteria:   EBESCT00000065062
- GeneID:   6144841
- GenomeReviews:   CP000970_GR
- KEGG:   ecm:EcSMS35_1095
- GeneTree:   EBGT00050000008982
- HOGENOM:   HBG705576
- OMA:   ATEELHY
- ProtClustDB:   PRK14017
- BioCyc:   ECOL439855:ECSMS35_1095-MONOMER
- HAMAP:   MF_01289
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.6

Molecular weight: Translated: 42550; Mature: 42550

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 185-185 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNSPVWQLMGGLVRDKIKAYSWV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
SKNAPDWRNPLWRHEDNSVAEW
CCCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAAVHELGDYLIGQDPSRI
CCCEEEEEEECCCCEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHH
NDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNSPVWQLMGGLVRDKIKAYSWV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
GGDRPANVIDGIKILREIGFDTFKLNGCEELGLIDNSRAVDAAVNTVAQIREAFGNQIEF
CCCCCHHHHHHHHHHHHCCCCEEEECCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEE
GLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAEYYPKLAAQTHIPLAAGERMFSR
CCCCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
FDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAEAYDVTLAPHCPLGPIALAACLHID
HHHHHHHHHCCCEEECCCHHHCCCHHHHHHHHCCCHHEEEEECCCCCCHHHHHHHHHHHH
FVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFSMVGGFFKPLTKPGLGVEIDEAKVIEF
HHHHHHHHHHHCCCEEECCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEECCCEEEEE
SKNAPDWRNPLWRHEDNSVAEW
CCCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA