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The map label for this gene is sacT [H]

Identifier: 170680940

GI number: 170680940

Start: 3035306

End: 3036130

Strand: Reverse

Name: sacT [H]

Synonym: EcSMS35_2962

Alternate gene names: 170680940

Gene position: 3036130-3035306 (Counterclockwise)

Preceding gene: 170681690

Following gene: 170683282

Centisome position: 59.9

GC content: 34.18

Gene sequence:

>825_bases
ATGATCATCGAAAAAGTCATGAACAATAATTGTGTACAGGCATCGATGAATGGACAGGAGGTTATCATTTCTGGGCCTGG
CGTCGGTTACAACAAAAAATATGGAATGTCGGTCCCTGAGCATCCGGCTAACCGGATTTTTTATGTCAGAAATGAACAAA
AAAATAAACTCTACAAATTGATTGAACATGTGGATATTGAGTATGTTTTTGTTGCCGAAAAAATAGTGCAATATGCGGAG
AACAATCTCGAAAAAAATCTCAATCCATCGCTACTATTGATTCTTGCTGATCACATTTCGAATGCAATATCCCGAGTCGC
TTCAGGCATACAAATTAATAATGTTTTCCTTGATGAAATCAAAGCGTTGTACAAAGCAGAGTATGCGATAAGTCGCGATG
CATTAACTATCATTAATGAGCAATTCAGCGTTCAACTTCCTGATGATGAGATTGGTTTTATAGCATTGCATATTTTAAAT
AATTATGAAAATTCAGTTGATTATGAATCAGTACGGATTATTGAGTTGTCGCAAATAATCACGGGGCTTATTGAAGTTGT
TTATAACAGAAAGGTGGACAGAAGTTCATTTAATTATTCCAGGTTCATGATACACCTTAAATATTTTTCAAGTCGCGTAT
TATGTAATGAAAAGATAAAACAAAAAGATATTGGTGACATCTATGAGCAGTTTCTTGAGAAAGACATCCTGTTACAGCGC
GCAATTCATGAAATTGAACGGTATCTGTATGCCACTTTTAAATATGAATTAATTTTAGAAGAAAAACTATATCTCTCAAT
TCGTACCAAAGTATTAATGGACTAA

Upstream 100 bases:

>100_bases
CGCATTAGTTAATCATCTTGTAACTGTTAAATCAGGCAAGGCAATGTTTGAAGTAATCATTACTTCTGACGTGCCTTGCC
TTTTTTTTTGGAGCCATGAA

Downstream 100 bases:

>100_bases
TTATATATAACATTTATATGAGATAACCCACATGAAACAAAAGAAAGCCTGGAGTTTTTTTCAGAGCCTGGGAAAGGCAT
TCATGTATCCCATTGCTCTG

Product: putative transcriptional antiterminator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPANRIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAE
NNLEKNLNPSLLLILADHISNAISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEKIKQKDIGDIYEQFLEKDILLQR
AIHEIERYLYATFKYELILEEKLYLSIRTKVLMD

Sequences:

>Translated_274_residues
MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPANRIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAE
NNLEKNLNPSLLLILADHISNAISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEKIKQKDIGDIYEQFLEKDILLQR
AIHEIERYLYATFKYELILEEKLYLSIRTKVLMD
>Mature_274_residues
MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPANRIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAE
NNLEKNLNPSLLLILADHISNAISRVASGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
NYENSVDYESVRIIELSQIITGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEKIKQKDIGDIYEQFLEKDILLQR
AIHEIERYLYATFKYELILEEKLYLSIRTKVLMD

Specific function: Mediates positive regulation of the sacPA operon by functioning as an antiterminator factor of transcription [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PRD domains [H]

Homologues:

Organism=Escherichia coli, GI1790160, Length=225, Percent_Identity=29.7777777777778, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004341
- InterPro:   IPR011608
- InterPro:   IPR001550 [H]

Pfam domain/function: PF03123 CAT_RBD; PF00874 PRD [H]

EC number: NA

Molecular weight: Translated: 31933; Mature: 31933

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPANRIFYVRNEQKNKLYKL
CCCHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
IEHVDIEYVFVAEKIVQYAENNLEKNLNPSLLLILADHISNAISRVASGIQINNVFLDEI
HHHCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCEEEHHHHHHH
KALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNNYENSVDYESVRIIELSQII
HHHHHHHHHHCCCHHEEECCCEEEECCCCCCCEEEEEEHHCCCCCCCCCCEEEEHHHHHH
TGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEKIKQKDIGDIYEQFLEKDILLQR
HHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AIHEIERYLYATFKYELILEEKLYLSIRTKVLMD
HHHHHHHHHHHHHHEEEEEHHHHHEEEHHHEECC
>Mature Secondary Structure
MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPANRIFYVRNEQKNKLYKL
CCCHHHHCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHH
IEHVDIEYVFVAEKIVQYAENNLEKNLNPSLLLILADHISNAISRVASGIQINNVFLDEI
HHHCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCEEEHHHHHHH
KALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNNYENSVDYESVRIIELSQII
HHHHHHHHHHCCCHHEEECCCEEEECCCCCCCEEEEEEHHCCCCCCCCCCEEEEHHHHHH
TGLIEVVYNRKVDRSSFNYSRFMIHLKYFSSRVLCNEKIKQKDIGDIYEQFLEKDILLQR
HHHHHHHHCCCCCCCCCCHHHHEEEHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
AIHEIERYLYATFKYELILEEKLYLSIRTKVLMD
HHHHHHHHHHHHHHEEEEEHHHHHEEEHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]