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The map label for this gene is yfkN [H]

Identifier: 170680658

GI number: 170680658

Start: 4473823

End: 4475373

Strand: Reverse

Name: yfkN [H]

Synonym: EcSMS35_4386

Alternate gene names: 170680658

Gene position: 4475373-4473823 (Counterclockwise)

Preceding gene: 170683638

Following gene: 170683900

Centisome position: 88.3

GC content: 40.75

Gene sequence:

>1551_bases
ATGAGAACTGTATTTACGACTTTATCAGTCATATTCAGCGTGATGTTTTCTCACAGTATACTAGCTCAGGACGTTACTAT
TTATTATACGAATGATATTCACGCACATGTCAATCCGGCAAAAATTCCCGCTGTTGATAAGAACAGACTTGTAGGTGGTA
TGGCTAATATTGCAGGCATCGTTAATGAAGCGAAGAAAAAAAACAAAGATGTCTTTTTCTTTGATGCAGGCGACTATTTT
ACCGGTCCGTATATCAGTACCCTGACCAAAGGTGAAGCTATTATTGATATCATGAACACCATGCCCTTTGATGCGGTGTC
CGTTGGTAATCACGAGTTCGATCACGGCGTGCCCAATATGGTATCTCAGTTATCAAAAGCAAAATTCCCCATTCTGCTGG
GAAATATTTACTACACCGATACAAATAAACCAGTTTGGGATCATCCGTGGACCATTATCGAAAAAGATGGTTTAAAGATT
GGTGTGATTGGATTGCATGGTGCATTCGCTTTCTATGATACAGTCGCGGCAAAAGCACGTGAAGGCGTAGAAGCCAGAGA
CGAAATTAAATATTTAAATAAAGCACTTGCAGAATTAAAAGGTAAAGTCGACATTACCGTTTTGCTAATTCACGAAGGTG
TTCCGGCGCGTCAATCAAGCTTTGGTAGTAAAGATGTGGAGCGGCTACTACAGGCAGATATTGAGACAGCTAAAAAAGTG
AATGGTGTTGATGTCTTAATTACCGGTCACGCACATGTCGGAACACCGCAACCAATAAAAGTTAATAACACATTAATTGT
ATCTACCGATGCATACGGCACCGACATCGGAAAACTTGTGCTTGATTACAATCCGAAAACAAAGAAAATTGATAGTTATA
ATGGTGAGTTAATCACCATCTTTGCAGATCAATTTAAACCTGACACCATCGTTCAAAATACTATCGATAAATGGAGCGTA
AAGCTTAACAAAATAACACAGGAAGTTGTCGGCCATTCTCCCGTAGTTTTAACACGTGAGTATGGCAGTTCTTCTTCTAC
CGGTAATCTTATTCTGGATGCAATGATGGAAAAAACACCTGATGCCATTGCCGGATTTCAAAATAGCGGTGGGATGCGAG
CTGATTTTCCTAAAGGTGATATCACACTGGGAGATGTTATTAGCACATTCCCCTTTAATAATGACCTCATCGAGATGGAT
TTGACGGGCCGCGATCTCAAATCGTTGATGACGCATGCAACCAATCTAACTAACGGTGTGTTACAGGTTTCAAAAAGCGT
TGCGGTTGTCTATGACAGCAAAAAACCACTCAACCAACGGTTAATCTCTTTCACCATTAACGGCAAACCCGTGGAAGATA
ATCAAACATATCGTATTGCCACGCACTCCTTTTGTGCCAGTGGTGGTGACGGTTTTGAAGCATTTTTGAATGGAAAAAAT
GTGAAGACGATACCGGGAACAACCTCGGCGGAATCTATCATCGATTATTTCAAAAATCATAAACCTGTCACCCCAGACTT
AACTAAACGAGTCATGGACGTCGCCAAATAA

Upstream 100 bases:

>100_bases
CATAATACTTTCCTGACCTATGCTTACATAAAAATAAGACACGGTGTTCCTTTTATTTAAATAATTCAACCAATGTAACA
ACCCCTATCAAGGAGGGATG

Downstream 100 bases:

>100_bases
AATATCATTTATCAGTCTATATATTTAACAAGGAATGCGAATGAAAACAGTGCAGCGTACTTTACTTGCCGCAACATTGC
TGACTATTTTTTCGGTCTCA

Product: serine/threonine protein phosphatase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPAVDKNRLVGGMANIAGIVNEAKKKNKDVFFFDAGDYF
TGPYISTLTKGEAIIDIMNTMPFDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQSSFGSKDVERLLQADIETAKKV
NGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDIGKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSV
KLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTPDAIAGFQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMD
LTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKN
VKTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK

Sequences:

>Translated_516_residues
MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPAVDKNRLVGGMANIAGIVNEAKKKNKDVFFFDAGDYF
TGPYISTLTKGEAIIDIMNTMPFDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQSSFGSKDVERLLQADIETAKKV
NGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDIGKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSV
KLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTPDAIAGFQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMD
LTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKN
VKTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK
>Mature_516_residues
MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPAVDKNRLVGGMANIAGIVNEAKKKNKDVFFFDAGDYF
TGPYISTLTKGEAIIDIMNTMPFDAVSVGNHEFDHGVPNMVSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKI
GVIGLHGAFAFYDTVAAKAREGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQSSFGSKDVERLLQADIETAKKV
NGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDIGKLVLDYNPKTKKIDSYNGELITIFADQFKPDTIVQNTIDKWSV
KLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTPDAIAGFQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMD
LTGRDLKSLMTHATNLTNGVLQVSKSVAVVYDSKKPLNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKN
VKTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=552, Percent_Identity=25.5434782608696, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786687, Length=558, Percent_Identity=25.089605734767, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1790658, Length=272, Percent_Identity=25.7352941176471, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19922446, Length=564, Percent_Identity=26.241134751773, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24654424, Length=564, Percent_Identity=26.241134751773, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI19922444, Length=562, Percent_Identity=24.7330960854093, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24641187, Length=546, Percent_Identity=25.8241758241758, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI221329836, Length=547, Percent_Identity=23.7659963436929, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI28573524, Length=547, Percent_Identity=24.1316270566728, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24652512, Length=473, Percent_Identity=24.7357293868922, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19921980, Length=473, Percent_Identity=24.7357293868922, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI161076508, Length=473, Percent_Identity=24.7357293868922, Blast_Score=115, Evalue=5e-26,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 56495; Mature: 56495

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00482 DIHYDROOROTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPAVDKNRLVGGMANIAGI
CCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCHHCCCCCCCHHHHHHHHHHHH
VNEAKKKNKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMPFDAVSVGNHEFDHGVPNM
HHHHHHCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHH
VSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKIGVIGLHGAFAFYDTVAAKAR
HHHHHHCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHH
EGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQSSFGSKDVERLLQADIETAKKV
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHC
NGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDIGKLVLDYNPKTKKIDSYNGELITI
CCEEEEEECCCCCCCCCCEEECCEEEEECCCCCCCCEEEEEECCCCCCEECCCCCCEEEE
FADQFKPDTIVQNTIDKWSVKLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTP
EECCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
DAIAGFQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMDLTGRDLKSLMTHATNLTNGV
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LQVSKSVAVVYDSKKPLNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKN
HHHCCCEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEEEHHHHCCCCCCHHHHCCCCC
VKTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK
EEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRTVFTTLSVIFSVMFSHSILAQDVTIYYTNDIHAHVNPAKIPAVDKNRLVGGMANIAGI
CCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCHHCCCCCCCHHHHHHHHHHHH
VNEAKKKNKDVFFFDAGDYFTGPYISTLTKGEAIIDIMNTMPFDAVSVGNHEFDHGVPNM
HHHHHHCCCCEEEEECCCCCCCCHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHHHH
VSQLSKAKFPILLGNIYYTDTNKPVWDHPWTIIEKDGLKIGVIGLHGAFAFYDTVAAKAR
HHHHHHCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHH
EGVEARDEIKYLNKALAELKGKVDITVLLIHEGVPARQSSFGSKDVERLLQADIETAKKV
CCCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHHC
NGVDVLITGHAHVGTPQPIKVNNTLIVSTDAYGTDIGKLVLDYNPKTKKIDSYNGELITI
CCEEEEEECCCCCCCCCCEEECCEEEEECCCCCCCCEEEEEECCCCCCEECCCCCCEEEE
FADQFKPDTIVQNTIDKWSVKLNKITQEVVGHSPVVLTREYGSSSSTGNLILDAMMEKTP
EECCCCCCHHHHHCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHCCC
DAIAGFQNSGGMRADFPKGDITLGDVISTFPFNNDLIEMDLTGRDLKSLMTHATNLTNGV
HHHHCCCCCCCCCCCCCCCCEEHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LQVSKSVAVVYDSKKPLNQRLISFTINGKPVEDNQTYRIATHSFCASGGDGFEAFLNGKN
HHHCCCEEEEECCCCCCCCEEEEEEECCEECCCCCEEEEEEHHHHCCCCCCHHHHCCCCC
VKTIPGTTSAESIIDYFKNHKPVTPDLTKRVMDVAK
EEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]