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The map label for this gene is yfkN [H]

Identifier: 170683900

GI number: 170683900

Start: 4472163

End: 4473782

Strand: Reverse

Name: yfkN [H]

Synonym: EcSMS35_4385

Alternate gene names: 170683900

Gene position: 4473782-4472163 (Counterclockwise)

Preceding gene: 170680658

Following gene: 170682215

Centisome position: 88.27

GC content: 44.26

Gene sequence:

>1620_bases
ATGAAAACAGTGCAGCGTACTTTACTTGCCGCAACATTGCTGACTATTTTTTCGGTCTCAGTCATGGCGCAGGATGTCAC
GATTATTTATACAAATGATCTTCACGCTCATGTGGATTCTTATAAAGTGCCGTATATCGCAGACGGAAAACGCGACATTG
GCGGCTTTGCCAATATATCTACTTTAGTAAAACAGGAGAAAGCGAAAAATAAAGCCACCTTTTATTTTGATGCTGGAGAC
TATTTCACCGGCCCTTATATCAGCAGTCTGACAAAGGGCGAAGCAATAATTGACATTATGAATACCATGCCTTTTGACGC
AGTATCAATCGGTAATCATGAGTTTGACCATGGCTGGGATAATGCCCTGCGACAATTAAGCAAAGCGAATTTTCCTGTTT
TATTGGGCAATGTTTATCATAAAGAGAGTGAGAACCCCTTCTGGAACAAGCCCTACACCATCCTGGAAAAAGATGGCATC
AAGATTGGTATTATTGGGCTACACGGCGTATTTGCATTTAATGATACGGTCTCGGAGCTCTCTCTCCAGGGACTCGATAA
TGACAATAATAACCGCTTTGATAAATCTTCCGAGACCCTTAAGAATCAGGGGATTGAAGCACGTGATGAAGTGAAGTATT
TGCAGCATTATATCGACGAGTTACGTGATAAGGTTGACCTCACTGTGGCGCTGGTTCATGAAGGCGTTCCGGCGCGTCAG
TCCAGCATTGGCAATACAGATGTTAGACGCGCTCTTGATAAAGATATTCAAACCGCTAGCAAGGTGAAAGGATTGGATAT
TCTGATTACCGGGCATGCCCACGTCGGAACGCCAGAACCAATCAAAGTCGGCAACACCTTAATCCTTTCAACTGATAGTG
GAGGTATCGATATTGGTAAATTAGTCCTTGATGTCAACCCTACAGCTCGTACCCATAAAATGAAGAGCTTTGAGCTGAAA
ACAGTTTATGCCGATGAATGGATACCCGATCCAACGACACAAAAAGTTATCAATGGCTGGAATAAAAAACTGGCGGATAT
TGTACGCCAACCAGTGGGTGAATCATCGATTGCATTAACTCGCGCCTATGGTGAGTCATCGCAACTAGGCAACCTGTTTA
CCGATGCAATGCTTGTCGCTGCTCCGACTGCACAGATTGCATTGATAAACTCCGGCAGCTTACGTGCGGATATAAATGCA
GGCACGATTACCTTTGGCGACATTACCAGTACATTCCCCTTCAAGAATGAACTTACTGAAATGGATCTCAGCGGTAAGGA
TCTGCGTAACCTACTGGAACACGGGGCATCGCTTACCAATGGCATCCTGCAAATGTCAAAAGGCGCAGAAATGCGCTATA
CCCCGCAGAAACCAGTAGGTCAGCGCATAGTATCTTTTAAAATTAATGGTGAAGAGATCGTTGATACGAATATTTACCAT
GTTGCAACAACGACTTTCCTTGCACTGGGGGGCGATGGTTTTCTGGCATTCAAAGAAGGGAAAAATGTTCAGGTCCGCGC
CGGAAATAACATGTCCGATGTCGTGATCGATTATTTGAAGAAAGGCCACAAGATTACGCCTGCACAGGTAAATGAAATGC
GGGTGGACGTTAGCAAATAA

Upstream 100 bases:

>100_bases
AAAAATCATAAACCTGTCACCCCAGACTTAACTAAACGAGTCATGGACGTCGCCAAATAAAATATCATTTATCAGTCTAT
ATATTTAACAAGGAATGCGA

Downstream 100 bases:

>100_bases
TAAAAAAATTATAATGCCATTCATTCACTGAATGGCATTATTTTCTCTAAATCAACAATAGATAATGAGATATATTTATG
TGTATAAAAAAGCACGGGAT

Product: serine/threonine protein phosphatase family protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 539; Mature: 539

Protein sequence:

>539_residues
MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDAGD
YFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGI
KIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQ
SSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFELK
TVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINA
GTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTPQKPVGQRIVSFKINGEEIVDTNIYH
VATTTFLALGGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK

Sequences:

>Translated_539_residues
MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDAGD
YFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGI
KIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQ
SSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFELK
TVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINA
GTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTPQKPVGQRIVSFKINGEEIVDTNIYH
VATTTFLALGGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK
>Mature_539_residues
MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANISTLVKQEKAKNKATFYFDAGD
YFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWDNALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGI
KIGIIGLHGVFAFNDTVSELSLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQ
SSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGKLVLDVNPTARTHKMKSFELK
TVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINA
GTITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTPQKPVGQRIVSFKINGEEIVDTNIYH
VATTTFLALGGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=579, Percent_Identity=25.3886010362694, Blast_Score=119, Evalue=5e-27,
Organism=Escherichia coli, GI1790658, Length=631, Percent_Identity=22.9793977812995, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786687, Length=183, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24641187, Length=537, Percent_Identity=26.0707635009311, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24652512, Length=489, Percent_Identity=25.9713701431493, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI161076508, Length=489, Percent_Identity=25.9713701431493, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19921980, Length=489, Percent_Identity=25.9713701431493, Blast_Score=133, Evalue=3e-31,
Organism=Drosophila melanogaster, GI19922446, Length=588, Percent_Identity=25.8503401360544, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24654424, Length=588, Percent_Identity=25.8503401360544, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI221329836, Length=561, Percent_Identity=25.1336898395722, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI28573524, Length=584, Percent_Identity=25.1712328767123, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922444, Length=583, Percent_Identity=23.6706689536878, Blast_Score=110, Evalue=3e-24,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 59221; Mature: 59221

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANIS
CCHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEHHCCCCCCEEECCCCCCCCHHHHH
TLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWD
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHH
NALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGIKIGIIGLHGVFAFNDTVSEL
HHHHHHHHCCCCEEECCEEECCCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCHHHH
SLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQ
EEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHH
SSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGK
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCEEEE
LVLDVNPTARTHKMKSFELKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALT
EEEECCCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNELTE
EECCCCCHHHHHHHHHHHEECCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCHHH
MDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTPQKPVGQRIVSFKINGEEIVDTNIYH
CCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCEEECCCEEE
VATTTFLALGGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK
EEEEEEEEECCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MKTVQRTLLAATLLTIFSVSVMAQDVTIIYTNDLHAHVDSYKVPYIADGKRDIGGFANIS
CCHHHHHHHHHHHHHHHHHHHEECCEEEEEECCCEEHHCCCCCCEEECCCCCCCCHHHHH
TLVKQEKAKNKATFYFDAGDYFTGPYISSLTKGEAIIDIMNTMPFDAVSIGNHEFDHGWD
HHHHHHHCCCCEEEEEECCCCCCCHHHHHHCCCHHHHHHHHCCCCCEEECCCCCCCCCHH
NALRQLSKANFPVLLGNVYHKESENPFWNKPYTILEKDGIKIGIIGLHGVFAFNDTVSEL
HHHHHHHHCCCCEEECCEEECCCCCCCCCCCCEEEECCCCEEEEEEEEEEEEECCCHHHH
SLQGLDNDNNNRFDKSSETLKNQGIEARDEVKYLQHYIDELRDKVDLTVALVHEGVPARQ
EEECCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCHH
SSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDIGK
HCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCEEEE
LVLDVNPTARTHKMKSFELKTVYADEWIPDPTTQKVINGWNKKLADIVRQPVGESSIALT
EEEECCCCHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEE
RAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAGTITFGDITSTFPFKNELTE
EECCCCCHHHHHHHHHHHEECCCEEEEEEECCCEEEECCCCEEEECCCCCCCCCCCCHHH
MDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTPQKPVGQRIVSFKINGEEIVDTNIYH
CCCCHHHHHHHHHCCCHHHHHHHHHCCCCCEEECCCCCCCCEEEEEEECCCEEECCCEEE
VATTTFLALGGDGFLAFKEGKNVQVRAGNNMSDVVIDYLKKGHKITPAQVNEMRVDVSK
EEEEEEEEECCCEEEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]