Click here to switch to the map view.

The map label for this gene is ycaL [H]

Identifier: 170680466

GI number: 170680466

Start: 2226227

End: 2226991

Strand: Reverse

Name: ycaL [H]

Synonym: EcSMS35_2211

Alternate gene names: 170680466

Gene position: 2226991-2226227 (Counterclockwise)

Preceding gene: 170679985

Following gene: 170682193

Centisome position: 43.94

GC content: 50.07

Gene sequence:

>765_bases
ATGAAGAATACTAAATTACTGCTGGCGATTGCGGCCTCTGCAGCTTTACTGACAGGGTGTCAGAATACCCACGGTATTGA
TACCAATATGGCTATCAGCTCAGGTTTAAATGCCTATAAAGCAGCAACGTTAAGCGATGCTGATGCAAAAGCGATTGCCA
ATCAGGGCTGTGCCGAAATGGACAGCGGCAATCAAGTCGCAAGTAAATCCAGCAAGTACGGTAAACGTCTGGCAAAAATC
GCCAAAGCATTGGGTAACAATATTAATGGCACGCCGGTCAACTATAAGGTTTATATGACCAGCGACGTCAACGCATGGGC
GATGGCGAACGGCTGTGTTCGTGTCTACAGTGGCCTGATGGACATGATGAACGACAACGAAATTGAAGGCGTTCTGGGTC
ATGAACTGGGCCACGTCGCGTTGGGTCACTCGCTGGCTGAAATGAAAGCTTCTTATGCGATCGTTGCCGCACGTGATGCG
ATTTCCGCTACCAGCGGTGTGGCTTCCCAGCTTTCTCGCTCACAATTAGGTGATATCGCAGAAGGCGCTATCAATGCGAA
ATACTCCCGCGATAAAGAGTCCGAAGCAGATGATTTCTCCTTTGATCTGTTGAAGAAACGTGGCATCAGCACCCAGGGGC
TGGTTGGCAGCTTTGAAAAATTGGCCAGCCTGGATGGCGGTCGCACCCAGTCCATGTTTGACTCTCACCCACCATCAACA
GAGCGTGCGCAACACATCCGTGATCGTATCGCCTCTGGTAAGTAA

Upstream 100 bases:

>100_bases
TTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGTTTGTCAATGTAAGTTATTGATACATAATATTTATATATGA
TTAATCAACGGATGATTCAC

Downstream 100 bases:

>100_bases
ATCATTGTCATCTTTCGGGCTGGTCTTCTGCCAGCCCGCTATAATTGCGCAATAAATCCCCATCTGAATACAGACAAAAC
TGGTTTTTGCACACAACGTT

Product: M48B family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK

Sequences:

>Translated_254_residues
MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK
>Mature_254_residues
MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKI
AKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
ERAQHIRDRIASGK

Specific function: Unknown

COG id: COG0501

COG function: function code O; Zn-dependent protease with chaperone function

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M48B family [H]

Homologues:

Organism=Escherichia coli, GI87081800, Length=254, Percent_Identity=99.2125984251969, Blast_Score=515, Evalue=1e-147,
Organism=Escherichia coli, GI87082185, Length=252, Percent_Identity=56.7460317460317, Blast_Score=296, Evalue=6e-82,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001915 [H]

Pfam domain/function: PF01435 Peptidase_M48 [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 26737; Mature: 26737

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEM
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHCCCHHC
DSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLM
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
DMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCH
ERAQHIRDRIASGK
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKNTKLLLAIAASAALLTGCQNTHGIDTNMAISSGLNAYKAATLSDADAKAIANQGCAEM
CCCCEEEEHHHHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHCCCHHHHHHHHCCCHHC
DSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSDVNAWAMANGCVRVYSGLM
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHH
DMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDAISATSGVASQLSRSQLGDIA
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHH
EGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSFEKLASLDGGRTQSMFDSHPPST
HHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCH
ERAQHIRDRIASGK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 7836281 [H]