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The map label for this gene is insG [H]

Identifier: 170679985

GI number: 170679985

Start: 2227127

End: 2228455

Strand: Reverse

Name: insG [H]

Synonym: EcSMS35_2212

Alternate gene names: 170679985

Gene position: 2228455-2227127 (Counterclockwise)

Preceding gene: 170683917

Following gene: 170680466

Centisome position: 43.97

GC content: 53.8

Gene sequence:

>1329_bases
ATGCACATTGGACAGGCTCTTGATCTGGTATCCCGTTACGATTCTCTGCGTAACCCACTGACTTCTCTGGGGGATTACCT
CGACCCCGAACTCATCTCTCGTTGCCTTGCCGAATCAGGTACTGTAACGCTACGCAAGCGCCGTCTTCCCCTCGAAATGA
TGGTCTGGTGTATTGTTGGCATGGCGCTTGAGCGTAAAGAACCTCTTCACCAGATTGTGAATCGCCTGGACATCATGCTG
CCGGGCAATCGCCCCTTCGTTGCCCCCAGTGCCGTTATTCAGGTCCGCCAGCGCCTGGGAAGTGAGGCTGTCCGCCGCGT
GTTCACGAAAACAGCGCAGCTCTGGCATAACGCCACGCCGCATCCGCACTGGTGCGGCCTGACCCTGCTGGCCATCGATG
GTGTGTTCTGGCGCACACCGGATACACCAGAGAACGATGCAGCCTTCCCCCGCCAGACACATGCCGGGAACCCGGCGCTC
TACCCGCAGGTCAAAATGGTCTGCCAGATGGAACTGACCAGCCATCTGCTGACGGCTGCAGCCTTCGGCACGATGAAGAA
CAGCGAAAATGAGCTTGCTGAGCAACTTATAGAACAAACCGGCGATAACACTCTGACGTTAATGGATAAAGGTTATTACT
CACTGGGACTGTTAAATGCCTGGAGCCTGGCGGGAGAACACCGCCACTGGATGATACCTATCAGAAAGGGAGCGCAATAT
GAAGAGAGCAGAAAACTGGGTAAAGGCGATCATCTGGTGAAGCTGAAAACCAGCCCGCAGGCACGAAAAAAGTGGCCGGG
ACTGGGAAATGAAGTGACAGCCCGCCTGCTGACCGTGACGCGCAAAGGAAAAGTCTGCCATCTGCTGACGTCGATGACGG
ACGCCATGCGCTTCCCCGGAGGAGAAATGGCGGATCTGTACAGTCATCGCTGGGAAATCGAACTGGGATACAGGGAGATA
AAACAGACGATGCAACTGAGCAGGCTGACGCTGAGAAGTAAAAAACCGGAGCTTGTGGAGCAAGAGCTGTGGGGTGTCTT
ACTGGCTTATAATCTGGTGAGATATCAGATGATTAAAATGGCGGAATATCTGAAAGGTTACTGGCCGAATCAACTGAGTT
TCTCAGAATCATGTGGAATGGTGATGAGAATGCTGATGACATTGCAGGGCGCTTCACCGGGACGTATACCGGAGCTGATG
CGCGATCTTGCAAGTATGGGACAACTTGTGAAATTACCGACAAGAAGGGAAAGGGCCTTCCCGAGAGTGGTAAAGGAGAG
GCCCTGGAAATACCCCACAGCCCCGAAAAAGAGCCAGTCAGTTGCTTAA

Upstream 100 bases:

>100_bases
CGTCGGCACATTGCCGTAATGCCGATCAGTTAAGGATCAGTTGACCGATCCAGTGGCTGTGTAAGAATCCGGAAACGCTC
ACTTGTTTCCGGATTTTTTT

Downstream 100 bases:

>100_bases
CTGACTGGCATTACGGCACATTGCCGGCGTTTTTTTTCGGACCTTGTGAGTCATTTTGATTAATGGTAGCGTCGTTTGTC
AATGTAAGTTATTGATACAT

Product: IS4 transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQVRQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPIRKGAQY
EESRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEYLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Sequences:

>Translated_442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQVRQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPIRKGAQY
EESRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEYLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA
>Mature_442_residues
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIML
PGNRPFVAPSAVIQVRQRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPIRKGAQY
EESRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREI
KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEYLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELM
RDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQSVA

Specific function: Involved in the transposition of the insertion sequence IS4 [H]

COG id: COG3385

COG function: function code L; FOG: Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1790729, Length=442, Percent_Identity=98.6425339366516, Blast_Score=902, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR002559 [H]

Pfam domain/function: PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 50386; Mature: 50386

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQVRQRLGSEAVRRVFTKTAQLWHNATP
HHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAA
CCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPIRKGAQY
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCH
EESRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPG
HHHHCCCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
CCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEYLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
PRVVKERPWKYPTAPKKSQSVA
HHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTLRKRRLPLEMMVWCIVG
CCCHHHHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHH
MALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQVRQRLGSEAVRRVFTKTAQLWHNATP
HHHHHCCHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQMELTSHLLTAA
CCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRHWMIPIRKGAQY
HHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCH
EESRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCHLLTSMTDAMRFPG
HHHHCCCCCCCEEEECCCCHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
GEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKM
CCHHHHHHCCEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AEYLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRERAF
HHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHH
PRVVKERPWKYPTAPKKSQSVA
HHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6268937; 7610040; 9278503 [H]