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The map label for this gene is kpsT

Identifier: 170680342

GI number: 170680342

Start: 3306334

End: 3306993

Strand: Reverse

Name: kpsT

Synonym: EcSMS35_3234

Alternate gene names: 170680342

Gene position: 3306993-3306334 (Counterclockwise)

Preceding gene: 170681020

Following gene: 170679748

Centisome position: 65.25

GC content: 34.85

Gene sequence:

>660_bases
ATGATTAAGATTGAGAATTTGACGAAGTCTTATCGCACGCCCACTGGGCGGCATTATGTTTTTAAAAATTTGAATATTAT
TTTTCCTAAAGGCTATAACATTGCCCTGATTGGTCAAAATGGAGCCGGTAAATCAACTTTACTTCGGATAATTGGTGGTA
TAGATCGTCCTGATAGTGGGAACATTATTACAGAACATAAAATTTCATGGCCCGTTGGATTGGCTGGTGGATTTCAAGGA
AGTTTAACCGGTCGTGAAAATGTTAAATTTGTTGCCCGACTATATGCAAAACGCGATGAGTTAAATGAGAGGGTTGATTT
TGTTGAAGAGTTTTCCGAACTCGGAAAATATTTCGATATGCCCATTAAAACTTATTCTTCTGGCATGAGGTCAAGGTTAG
CTTTTGGATTAAGTATGGCTTTTAAATTCGACTATTATCTTATTGATGAAATCACTGCTGTTGGAGACGCAAAGTTTAAA
AAGAAATGTTCAGATATATTCGATAAAATAAGAGAAAAATCTCATTTAATAATGGTGTCACATAGTGAACGAGCTTTAAA
GGAGTATTGTGATGTTGCTATTTATCTTAACAAAGAGGGGCAAGGTAAATTTTATAAAAATGTTACGGAAGCCATTGCTG
ATTACAAAAAAGACCTATAG

Upstream 100 bases:

>100_bases
GTGAAGGCGTGAGTCTGAACTACCTTGCAATGTTTACTCTGGTCACCCTGTTCATCGGCCTAGCATTATACCGAACGCGT
GAAGAGGCAATGCTGACATC

Downstream 100 bases:

>100_bases
TGGTTACATTCCAATATTATGCCTTGGAAATATTTAACTGAGACATATCATGAGTAAAAAATTAATAATATTTGGTGCGG
GTGGTTTTTCAAAATCTATA

Product: polysialic acid capsule biosynthesis transport protein KpsT

Products: ADP; phosphate; maltose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKSTLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQG
SLTGRENVKFVARLYAKRDELNERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL

Sequences:

>Translated_219_residues
MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKSTLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQG
SLTGRENVKFVARLYAKRDELNERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL
>Mature_219_residues
MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKSTLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQG
SLTGRENVKFVARLYAKRDELNERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL

Specific function: Putative ATP-binding protein, and an energy coupling component for the transport of polysialic acid across the cytoplasmic membrane

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain

Homologues:

Organism=Homo sapiens, GI27881501, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI30795238, Length=219, Percent_Identity=26.4840182648402, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790467, Length=209, Percent_Identity=27.7511961722488, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI87081709, Length=210, Percent_Identity=24.2857142857143, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786398, Length=218, Percent_Identity=26.1467889908257, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI1788761, Length=216, Percent_Identity=25.9259259259259, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994943, Length=190, Percent_Identity=26.8421052631579, Blast_Score=68, Evalue=4e-13,
Organism=Drosophila melanogaster, GI221500365, Length=190, Percent_Identity=27.8947368421053, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI116007184, Length=190, Percent_Identity=27.8947368421053, Blast_Score=73, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPST1_ECOLX (P23888)

Other databases:

- EMBL:   M57381
- PIR:   B42469
- ProteinModelPortal:   P23888
- SMR:   P23888
- GO:   GO:0006810
- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- SMART:   SM00382

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 24939; Mature: 24939

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: PS00211 ABC_TRANSPORTER_1; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKSTLLRIIGGIDRPDSG
CEEECCCHHHHCCCCCCEEEEECCEEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
NIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELNERVDFVEEFSELGKYFDM
CEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFKKKCSDIFDKIREKSHLIMVS
CHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
HSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL
CCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGKSTLLRIIGGIDRPDSG
CEEECCCHHHHCCCCCCEEEEECCEEEECCCCEEEEEECCCCCHHHHHHHHCCCCCCCCC
NIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELNERVDFVEEFSELGKYFDM
CEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFKKKCSDIFDKIREKSHLIMVS
CHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEE
HSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKDL
CCHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; maltose [Periplasm]; H2O [C]

Specific reaction: ATP + maltose [Periplasm] + H2O = ADP + phosphate + maltose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1856162; 8051103