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The map label for this gene is kpsM

Identifier: 170681020

GI number: 170681020

Start: 3306990

End: 3307766

Strand: Reverse

Name: kpsM

Synonym: EcSMS35_3235

Alternate gene names: 170681020

Gene position: 3307766-3306990 (Counterclockwise)

Preceding gene: 170682393

Following gene: 170680342

Centisome position: 65.26

GC content: 43.76

Gene sequence:

>777_bases
ATGGCAAGAAGTGGATTTGAAGTCCAGAAAGTCACCGTAGAGGCATTATTTCTACGAGAAATACGAACACGCTTTGGTAA
GTTCCGTCTGGGATATTTGTGGGCGATTCTCGAACCCTCCGCGCATTTGCTGATACTGTTGGGAATTTTGGGTTACGTTA
TGCACCGCACTATGCCAGACATCTCGTTCCCGGTGTTTTTACTTAATGGCCTGATTCCCTTTTTTATCTTTAGTAGTATT
AGCAAACGTTCTATTGGTGCTATTGAAGCGAACCAGGGGCTGTTTAATTATCGACCAGTAAAACCCATCGATACGATCAT
TGCACGTGCACTGCTTGAGACACTGATTTACGTTGCTGTTTATATTTTGCTCATGCTTATCGTCTGGATGACAGGCGAAT
ATTTCGAAATTACAAACTTTTTACAACTTGTGCTCACCTGGAGTTTGTTAATCATTCTTTCATGTGGCGTCGGCTTAATA
TTTATGGTCGTTGGTAAAACCTTTCCTGAAATGCAAAAGGTCCTGCCGATACTGCTTAAGCCGCTGTATTTCATCTCCTG
CATCATGTTCCCTCTACACTCGATTCCAAAGCAATACTGGTCATATCTACTCTGGAACCCATTAGTGCATGTTGTGGAGT
TAAGCCGCGAGGCAGTTATGCCTGGCTATATCAGTGAAGGCGTGAGTCTGAACTACCTTGCAATGTTTACTCTGGTCACC
CTGTTCATCGGCCTAGCATTATACCGAACGCGTGAAGAGGCAATGCTGACATCATGA

Upstream 100 bases:

>100_bases
AAATTTTATCCAGTTACAAATAAGCATTACCTCCAGTGTATTGGTAGCTGTTAAGCCAAGGGCGGTAGCGTACCTGAAGA
GATTAGGATCACATCATCAA

Downstream 100 bases:

>100_bases
TTAAGATTGAGAATTTGACGAAGTCTTATCGCACGCCCACTGGGCGGCATTATGTTTTTAAAAATTTGAATATTATTTTT
CCTAAAGGCTATAACATTGC

Product: polysialic acid capsule biosynthesis transport protein KpsM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSI
SKRSIGAIEANQGLFNYRPVKPIDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLI
FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVT
LFIGLALYRTREEAMLTS

Sequences:

>Translated_258_residues
MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSI
SKRSIGAIEANQGLFNYRPVKPIDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLI
FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVT
LFIGLALYRTREEAMLTS
>Mature_257_residues
ARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSIS
KRSIGAIEANQGLFNYRPVKPIDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLIF
MVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVTL
FIGLALYRTREEAMLTS

Specific function: KpsM and kpsT constitute a system for the transport of polysialic acid across the cytoplasmic membrane

COG id: COG1682

COG function: function code GM; ABC-type polysaccharide/polyol phosphate export systems, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-2 domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPSM1_ECOLX (P23889)

Other databases:

- EMBL:   M57382
- PIR:   A42469
- InterPro:   IPR000412
- InterPro:   IPR013526
- InterPro:   IPR013525
- PRINTS:   PR00164

Pfam domain/function: PF01061 ABC2_membrane

EC number: NA

Molecular weight: Translated: 29558; Mature: 29427

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS51012 ABC_TM2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1bec5cb8)-; HASH(0x1bb8b908)-; HASH(0x1b6eb844)-; HASH(0x1c120330)-; HASH(0x1bc81468)-; HASH(0x19fd3cd4)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLILLGILGYVMHRTMPD
CCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKPIDTIIARALLETLIYVAV
CCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLIFMVVGKTFPEMQKVLPILLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHH
LFIGLALYRTREEAMLTS
HHHHHHHHHHHHHHHCCH
>Mature Secondary Structure 
ARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLLILLGILGYVMHRTMPD
CCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
ISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKPIDTIIARALLETLIYVAV
CCHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
YILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLIFMVVGKTFPEMQKVLPILLK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
PLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREAVMPGYISEGVSLNYLAMFTLVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHH
LFIGLALYRTREEAMLTS
HHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1856162; 7715449